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| Madec, Stephanie; Pichereau, Vianney; Jacq, Annick; Paillard, Mathieu; Boisset, Claire; Guerard, Fabienne; Paillard, Christine; Nicolas, Jean-louis. |
| Vibrio tapetis causes the brown ring disease in the Japanese clam Ruditapes philippinarum while Vibrio aestuarianus is associated with massive oyster mortalities. As extracellular proteins are often associated with the virulence of pathogenic bacteria, we undertook a proteomic approach to characterize the secretomes of both vibrios. The extracellular proteins (ECPs) of both species were fractionated by SEC-FPLC and in vitro assays were performed to measure the effects of each fraction on hemocyte cellular parameters (phagocytosis and adhesion). Fractions showing a significant effect were subjected to SDS-PAGE, and proteins were identified by nano LC-MS/MS. 45 proteins were identified for V. aestuarianus and 87 for V. tapetis. Most of them belonged to outer... |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00242/35334/34576.pdf |
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| Lelchat, Florian; Cozien, Joelle; Le Costaouec, Tinaig; Brandily, Christophe; Schmitt, Sophie; Baudoux, Anne-claire; Colliec-jouault, Sylvia; Boisset, Claire. |
| Alteromonas macleodii subsp. fijiensis biovar deepsane is a deep-sea ecotype exopolysaccharide-producing bacteria isolated from the polychaete annelid Alvinella pompejana. The high molecular weight biopolymer HYD657 produced by this strain, is the first marine exopolysaccharide (EPS) to be commercialized for cosmetic use. Depolymerization methods are necessary to elucidate the complete structure of this EPS and to generate potentially bioactive oligosaccharides. Enzymatic methods are useful for elucidating polysaccharide structure because they specifically cleave glycosidic bonds and do not require harsh chemical conditions. The HYD657 EPS is structurally complex and no commercially available enzymes are able to effectively degrade it. Here, we present the... |
| Tipo: Text |
Palavras-chave: Marine Alteromonas macleodii sp strain; Bacterial exopolysaccharides; Microbial extracellular enzymes; Endogenous enzymatic degradation. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00240/35129/33893.pdf |
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| Courtois, Anthony; Berthou, Christian; Guezennec, Jean; Boisset, Claire; Bordron, Anne. |
| The complement system is involved in the defence against bacterial infection, or in the elimination of tumour cells. However, disturbances in this system contributes to the pathogenesis of various inflammatory diseases. The efficiency of therapeutic anti-tumour antibodies is enhanced when the complement system is stimulated. In contrast, cancer cells are able to inhibit the complement system and thus proliferate. Some marine molecules are currently being developed as new drugs for use in humans. Among them, known exopolyssacharides (EPSs) generally originate from fungi, but few studies have been performed on bacterial EPSs and even fewer on EPSs extracted from deep-sea hydrothermal vent microbes. For use in humans, these high molecular weight EPSs must be... |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00198/30963/29337.pdf |
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| Courtois, Anthony; Simon-colin, Christelle; Boisset, Claire; Berthou, Christian; Deslandes, Eric; Guezennec, Jean; Bordron, Anne. |
| Many biological properties of algae have been found to have useful applications in human health, particularly in the fields of oncology and immunology. Floridoside, extracted from the red alga Mastocarpus stellatus, has a structure similar to the xenoantigen Gal alpha 1-3 Gal. This xenoantigen has been described to induce a high immune response in human xenografts and is mediated by natural anti-gal antibodies that activate the classical complement pathway. Based on this property, we analyzed the potential activities of floridoside on the immune system. We demonstrated that floridoside activates a complement cascade via the classical complement pathway, through the recruitment and activation of natural IgM. This algal molecule could represent an important... |
| Tipo: Text |
Palavras-chave: Complement system; Immunomodulation; Marine algae; Alpha galactosyl glycerol. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6248.pdf |
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| Tall, Amadou; Teillon, Anna; Boisset, Claire; Delesmont, R.; Touron-bodilis, A.; Hervio-heath, Dominique. |
| Aims: To identify Vibrio vulnificus, Vibrio cholerae and Vibrio alginolyticus using standardized DNA extraction method and real-time PCR assays, among a large number of bacterial strains isolated from marine environment. Methods and Results: Methods for DNA extraction and real-time PCR were standardized to identify a large number of Vibrio spp. strains isolated through regular collection campaigns of environmental samples. Three real-time PCR assays were developed from a multiplex PCR, targeting V. vulnificus, V. cholerae and V. alginolyticus on the dnaJ gene. After testing their specificity, these systems were applied for the identification of 961 strains isolated at 22°C (446 strains) and 37°C (515 strains) in September 2009. The predominance of V.... |
| Tipo: Text |
Palavras-chave: DnaJ; Identification; Real-time PCR; Vibrio alginolyticus; Vibrio cholerae; Vibrio vulnificus. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19901/17562.pdf |
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| Le Costaouec, Tinaig; Cerantola, Stephane; Ropartz, D.; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Boisset, Claire. |
| Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharide; Alteromonas macleodii; Structure determination; NMR spectroscopy; Mass spectrometry. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00095/20620/18536.pdf |
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| Lelchat, Florian; Cerantola, Stephane; Brandily, Christophe; Colliec-jouault, Sylvia; Baudoux, Anne-claire; Ojima, Takao; Boisset, Claire. |
| We have studied the exopolysaccharide produced by Cobetia marina DSMZ 4741, a marine bacterium isolated from coastal seawater. This strain is able to produce a polysaccharide in presence of carbon sources as glucose, mannitol and alginate. The maximum production occurs in aerobic condition, during the end of the exponential phase. The polymer is a non-viscous, acidic heteropolysaccharide of 270 kDa constituted of a repeating unit of: →2)-β-D-Ribf-(1→4)-[7,8-O-(Pyr)]-α-D-KDOp-(2→ This kind of chemical structure is generally related to K-antigen polysaccharide of pathogenic Escherichia coli strains. This is the first time this type of EPS is described from a marine bacterium. Moreover the polysaccharide exhibits a pyruvate substitution on its... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharides; K-antigen; Cobetia marina; KDO; Marine polysaccharide. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00254/36494/35038.pdf |
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| Lelchat, Florian; Mocaer, P Y; Ojima, T; Michel, G; Sarthou, Geraldine; Bucciarelli, Eva; Cérantola, S; Colliec-jouault, Sylvia; Boisset, Claire; Baudoux, A-c. |
| The identification of the mechanisms by which marine dissolved organic matter (DOM) is produced and regenerated is critical to develop robust prediction of ocean carbon cycling. Polysaccharides represent one of the main constituents of marine DOM and their degradation is mainly attributed to polysaccharidases derived from bacteria. Here, we report that marine viruses can depolymerize the exopolysaccharides (EPS) excreted by their hosts using 5 bacteriophages that infect the notable EPS producer, Cobetia marina DSMZ 4741. Degradation monitorings as assessed by gel electrophoresis and size exclusion chromatography showed that 4 out of 5 phages carry structural enzymes that depolymerize purified solution of Cobetia marina EPS. The depolymerization patterns... |
| Tipo: Text |
Palavras-chave: Marine phage; EPS; Polysaccharidase; DOM; Ocean. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00499/61062/64512.pdf |
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