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A unifying quantitative framework for exploring the multiple facets of microbial biodiversity across diverse scales ArchiMer
Escalas, Arthur; Bouvier, Thierry; Mouchet, Maud A.; Leprieur, Fabien; Bouvier, Corinne; Troussellier, Marc; Mouillot, David.
Recent developments of molecular tools have revolutionized our knowledge of microbial biodiversity by allowing detailed exploration of its different facets and generating unprecedented amount of data. One key issue with such large datasets is the development of diversity measures that cope with different data outputs and allow comparison of biodiversity across different scales. Diversity has indeed three components: local (), regional () and the overall difference between local communities (). Current measures of microbial diversity, derived from several approaches, provide complementary but different views. They only capture the component of diversity, compare communities in a pairwise way, consider all species as equivalent or lack a mathematically...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00163/27441/25668.pdf
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Coral-associated viruses and bacteria in the Ha Long Bay, Vietnam ArchiMer
Thu Pham, The; Thuoc Chu, Van; Viet Ha Bui, Thi; Thuy Nguyen, Thanh; Huy Tran, Quang; Ngoc Mai Cung, Thi; Bouvier, Corinne; Brune, Justine; Villeger, Sebastien; Bouvier, Thierry; Bettarel, Yvan.
Viruses inhabiting the surface mucus layer of scleractinian corals have received little ecological attention so far. Yet they have recently been shown to be highly abundant and could even play a pivotal role in coral health. A fundamental aspect that remains unresolved is whether their abundance and diversity change with the trophic state of their environment. The present study examined the variability in the abundance of viral and bacterial epibionts on 13 coral species collected from 2 different sites in the Ha Long Bay, Vietnam: one station heavily affected by anthropogenic activity (Cat Ba Island) and one protected offshore station (Long Chau Island). In general, viral abundance was significantly higher in coral mucus (mean = 10.6 +/- 2.0 x 10(7)...
Tipo: Text Palavras-chave: Viruses; Coral-associated bacteria; Mucus; Symbionts; Coral reefs.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00627/73870/73680.pdf
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Exceptional but vulnerable microbial diversity in coral reef animal surface microbiomes ArchiMer
Chiarello, Marlène; Auguet, Jean-christophe; Graham, Nicholas A. J.; Claverie, Thomas; Sucré, Elliott; Bouvier, Corinne; Rieuvilleneuve, Fabien; Restrepo-ortiz, Claudia Ximena; Bettarel, Yvan; Villéger, Sébastien; Bouvier, Thierry.
Coral reefs host hundreds of thousands of animal species that are increasingly threatened by anthropogenic disturbances. These animals host microbial communities at their surface, playing crucial roles for their fitness. However, the diversity of such microbiomes is mostly described in a few coral species and still poorly defined in other invertebrates and vertebrates. Given the diversity of animal microbiomes, and the diversity of host species inhabiting coral reefs, the contribution of such microbiomes to the total microbial diversity of coral reefs could be important, yet potentially vulnerable to the loss of animal species. Analysis of the surface microbiome from 74 taxa, including teleost fishes, hard and soft corals, crustaceans, echinoderms,...
Tipo: Text Palavras-chave: Skin microbiota; Phylogenetic diversity; Conservation; Marine biodiversity; Octocorallia; Scleratinia.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00629/74121/73596.pdf
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High diversity of skin-associated bacterial communities of marine fishes is promoted by their high variability among body parts, individuals and species ArchiMer
Chiarello, Marlene; Villeger, Sebastien; Bouvier, Corinne; Bettarel, Yvan; Bouvier, Thierry.
Animal-associated microbiotas form complex communities, which are suspected to play crucial functions for their host fitness. However, the biodiversity of these communities, including their differences between host species and individuals, has been scarcely studied, especially in case of skin-associated communities. In addition, the intraindividual variability (i.e. between body parts) has never been assessed to date. The objective of this study was to characterize skin bacterial communities of two teleostean fish species, namely the European seabass (Dicentrarchus labrax) and gilthead seabream (Sparus aurata), using a high-throughput DNA sequencing method. In order to focus on intrinsic factors of host-associated bacterial community variability,...
Tipo: Text Palavras-chave: Sparus aurata; Dicentrarchus labrax; Skin microbiome; Next generation sequencing; Phylogenetic diversity.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00277/38801/37547.pdf
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Interspecific differences in the effect of fish on marine microbial plankton ArchiMer
Villeger, Sebastien; Fouilland, Eric; Argenty, Jeremy; Bouvier, Corinne; Carre, Claire; Bouvier, Thierry.
The productivity of most marine ecosystems is limited by the availability of dissolved nitrogen (N) and phosphorus (P). Nutrient recycling is therefore a key process for ecosystem functioning. Fish recycle nutrients through the excretion of ammonia and phosphate and can influence the abundance and community structure of primary producers such as phytoplankton. However, the effect of fish on other plankton compartments, and whether all fish species have similar effects, is largely unknown. We used a tank experiment to test how 2 Mediterranean fish species, gilthead seabream Sparus aurata and golden mullet Chelon auratus, with distinctly different N and P excretion rates, can affect the abundance and community structure of 3 plankton compartments:...
Tipo: Text Palavras-chave: Marine fish; Nutrient excretion; Protozooplankton; Phytoplankton; Bacteria.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00584/69659/67585.pdf
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Jellyfish as an alternative source of food for opportunistic fishes ArchiMer
Marques, Raquel; Bouvier, Corinne; Darnaude, Audrey M.; Molinero, Juan-carlos; Przybyla, Cyrille; Soriano, Solenn; Tomasini, Jean-antoine; Bonnet, Delphine.
Although scientific interest on jellyfish ecology has substantially increased in the last decades, little is known on the role of potential predators shaping their population dynamics. Jellyfish were long considered as ‘dead ends’ within food webs, and therefore overlooked as potential food source for higher trophic levels, e.g. fishes. Here this question is tackled by using comprehensive laboratory experiments assessing fish predation on jellyfish. The approach included all the life stages (polyps, ephyrae and medusa) of Aurelia sp. versus more traditional aquaculture feeds in an easily farmed opportunistic fish, the gilthead seabream Sparus aurata (L.). Results revealed that all life stages of Aurelia sp. were accepted as a source of food by S. aurata,...
Tipo: Text Palavras-chave: Aurelia sp.; Predation; Fish; Sparus aurata; Ingestion rate; Energy pathways.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00349/46018/46026.pdf
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Microbial food web structural and functional responses to oyster and fish as top predators ArchiMer
Mostajir, Behzad; Roques, Cecile; Bouvier, Corinne; Bouvier, Thierry; Fouilland, Eric; Got, Patrice; Le Floc'H, Emilie; Nouguier, Jean; Mas, Sebastien; Sempere, Richard; Sime-ngando, Telesphore; Troussellier, Marc; Vidussi, Francesca.
The impact of fish and oysters on components of the pelagic microbial food web (MFW) was studied in a 10 d mesocosm experiment using Mediterranean coastal waters. Two mesocosms contained natural water only ('Controls'), 2 contained natural water with Crassostrea gigas ('Oyster'), and 2 contained natural water with Atherina spp. ('Fish'). Abundances and biomasses of microorganisms (viruses, bacteria, phytoplankton, heterotrophic flagellates, and ciliates) were measured to estimate their contribution to the total microbial carbon biomass. Two MFW indices, the microbial autotroph: heterotroph C biomass ratio (A:H) structural index and the gross primary production: respiration ratio (GPP:R) functional index, were defined. In the Fish mesocosms, selective...
Tipo: Text Palavras-chave: Microbial food web; Virioplankton; Bacterioplankton; Phytoplankton; Protozooplankton; Crassostrea; Atherina; Autotrophy; Heterotrophy.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00284/39489/73675.pdf
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Molecular approach indicates consumption of jellyfish by commercially important fish species in a coastal Mediterranean lagoon ArchiMer
Marques, Raquel; Darnaude, Audrey M.; Crochemore, Sandrine; Bouvier, Corinne; Bonnet, Delphine.
Until recently, jellyfish have been ignored as an important source of food, due to their low nutritional value. Here, quantitative PCR was used to detect and quantify the DNA of the jellyfish Aurelia coerulea in the gut contents of commercially important fish species from the Thau Lagoon. Individuals from five fish species were collected during two different periods: the bloom period, when the pelagic stages of A. coerulea are abundant, and the post-bloom period, when only the benthic stage – polyps – is present in the lagoon. The DNA of A. coerulea was detected in the guts of 41.9% of the fish analysed, belonging to four different species. The eel Anguilla anguilla and the seabream Sparus aurata were important jellyfish consumers during the bloom and...
Tipo: Text Palavras-chave: Predation; Aurelia coerulea; Eel; Seabream; Polyps; Medusae; Quantitative PCR; Gut content; Thau lagoon.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00513/62469/66986.pdf
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Skin microbiome of coral reef fish is highly variable and driven by host phylogeny and diet ArchiMer
Chiarello, Marlene; Auguet, Jean-christophe; Bettarel, Yvan; Bouvier, Corinne; Claverie, Thomas; Graham, Nicholas A. J.; Rieuvilleneuve, Fabien; Sucre, Elliot; Bouvier, Thierry; Villeger, Sebastien.
Background: The surface of marine animals is covered by abundant and diversified microbial communities, which have major roles for the health of their host While such microbiomes have been deeply examined in marine invertebrates such as corals and sponges, the microbiomes living on marine vertebrates have received less attention. Specifically, the diversity of these microbiomes, their variability among species, and their drivers are still mostly unknown, especially among the fish species living on coral reefs that contribute to key ecosystem services while they are increasingly affected by human activities. Here, we investigated these knowledge gaps analyzing the skin microbiome of 138 fish individuals belonging to 44 coral reef fish species living in the...
Tipo: Text Palavras-chave: Tropical; Teleost; Microbiota; Phylogenetic diversity; Phylosymbiosis; Phylogenetic signal.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56565/74934.pdf
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Viral and Bacterial Epibionts in Thermally-Stressed Corals ArchiMer
Nguyen-kim, Hanh; Bouvier, Thierry; Bouvier, Corinne; Ngoc Bui, Van; Le-lan, Huong; Bettarel, Yvan.
The periodic rise in seawater temperature is one of the main environmental determinants of coral bleaching. However, the direct incidence of these episodic thermal anomalies on coral-associated microbiota and their subsequent effects on coral health are still not completely understood. In this study, we investigated the dynamics of three main microbial communities of the coral holobiont (e.g., Symbiodinium, bacteria and viruses), during an experimental thermal stress (+4 degrees C) conducted on the scleractinian Fungia repanda. The heat-treatment induced coral bleaching after 11 days and resulted in a final elevation of ca. 9, 130 and 250-fold in the abundance of mucosal viruses, bacteria, and Symbiodinium, respectively. On the contrary, the proportion of...
Tipo: Text Palavras-chave: Coral bleaching; Thermal stress; Bacteria; Viruses; Holobiont; Vietnam.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00626/73823/73642.pdf
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