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Registros recuperados: 19 | |
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COSTA, M. D. L.; REIS, P. A. B.; VALENTE, M. A.; IRSIGLER, A. S. T.; CARVALHO, C. M.; LOUREIRO, M. E.; ARAGÃO, F. J. L.; BOSTON, R. S.; FIETTO, L. G.; FONTES, E. P. B.. |
Tipo: Separatas |
Palavras-chave: Proteína. |
Ano: 2008 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/190419 |
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LIMA, A. M. de; SALEM, J. I.; SOUZA, J. V. B. de; CORTEZ, A. C. A.; CARVALHO, C. M.; CHAVES, F. C. M.; VEIGA JUNIOR, V. F. da. |
Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from... |
Tipo: Separatas |
Palavras-chave: Estimulação de crescimento.; Tuberculose.. |
Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/901384 |
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CARVALHO, S. L. de; SILVA, F. N. da; ZANARDO, L. G.; ALMEIDA, A. M. R.; ZERBINI, F. M.; CARVALHO, C. M.. |
Cowpea mild mottle virus (CpMMV), the causal agent of stem necrosis disease, has drawn attention of soybean producers in recent years due to yield losses in the main producing regions of Brazil. Serological methods for viral detection require the use of an antiserum of good quality to achieve specificity and sensitivity. The entire coat protein gene of a Brazilian CpMMV isolate was cloned into a bacterial expression vector and transformed into Escherichia coli BL21::DE3 for in vitro expression. The coat protein, fused to a His-tag, was purified under denaturing conditions by affinity chromatography using a Ni-NTA resin. After renaturation, the integrity and identity of the purified recombinant protein was confirmed by SDS-Page and MALDI-ToF/ToF mass... |
Tipo: Artigo de periódico |
Palavras-chave: Doença de planta; Vírus; Soja; Plant diseases and disorders; Cowpea mild mottle virus; Soybeans. |
Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/952212 |
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Registros recuperados: 19 | |
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