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| CLEMENTE, A. C. S.; GUIMARÃES, R. M.; MARTINS, D. C.; GOMES, L. A. A.; CAIXETA, F.; REIS, R. G. E.; ROSA, S. D. V. F. da. |
| Seed germination and dormancy are complex phenomena that are controlled by many genes and environmental factors. Such genes are indicated by phytohormones that interact with each other, and may cause dormancy or promote seed germination. The objective of this study was to investigate gene expression associated with the biosynthetic pathways of abscisic acid (ABA), gibberellic acid (GA), and ethylene (ET) in dormant and germinated lettuce seeds. The expressions of LsNCED, LsGA3ox1, and ACO-B were evaluated in germinating and dormant seeds from the cultivars Everglades, Babá de Verão, Verônica, Salinas, Colorado, and Regina 71. The expressions of LsNCED, LsGA3ox1, and ACO-B were related to the biosynthesis of ABA, GA, and ET, respectively; therefore, the... |
| Tipo: Artigo de periódico |
Palavras-chave: Lactuca Sativa.; Genetic techniques and protocols; Dormancy.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1039289 |
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| SANTOS, F. C. dos; ROSA, S. D. V. F. da; COELHO, S. V. B.; CLEMENTE, A. C. S.; FANTAZZINI, T. B.; PEREIRA, C. C.; CAIXETA, F.; FÁVARIS, N. A. B.; BAÚTE, J. L.. |
| During maturation in seeds, metabolic processes dependent on gene expression are controlled by hormones, including abscisic acid and gibberellin. These hormones determine dormancy or the capacity for germination in seeds and may have different expression levels in the endosperm and embryo of coffee seeds. We quantified gene expression in the biosynthetic pathway of gibberellin and abscisic acid in different parts of Coffea arabica L. seeds in pre and post physiological maturity, in order to better understand the germination mechanisms of this species. Coffee fruits were harvested at green, yellowish-green, cherry, over-ripe and dry stages. For studies of gene expression, intact seeds were used in addition to endosperm and isolated embryos. The RNA from... |
| Tipo: Artigo de periódico |
Palavras-chave: QRT-PCR; Coffea Arábica; Gibberellic acid; Abscisic acid. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114204 |
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| SANTOS, F. C.; CAIXETA, F.; CLEMENTE, A. C. S.; PINHO, E. V.; ROSA, S. D. V. F. da. |
| Seeds collected at different maturation stages vary in physiological quality and patterns of protective antioxidant systems against deterioration. In this study we investigated the expression of genes that codify catalase (CAT), dismutase superoxide (SOD), and polyphenol oxidase (PPO) during the pre- and post-physiological maturation phases in whole seeds and in endosperms and embryos extracted from the seeds. Coffea arabica L. berries were collected at the green, yellowish-green, cherry, over-ripe, and dry stages, and the seeds were examined physiologically. The transcription levels of the genes were quantified by quantitative real-time polymerase chain reaction using coffee-specific primers. The highest level of SOD expression was observed in the... |
| Tipo: Artigo de periódico |
Palavras-chave: Catalace.; Superoxide dismutase; Catechol oxidase.. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1039772 |
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| ABREU, G. F. de; ROSA, S. D. V. F. da; CIRILLO, M. A.; MALTA, M. R.; CLEMENTE, A. C. S.; BORÉM, F. M.. |
| The objective of the study was to use methodology of simultaneous optimization of multiple responses applied to an experimental design to determine the best combination of storage period and conditions for preservation of coffee beans. Coffea arabica L. fruits were harvested in the ripe stage of maturation, processed using wet and dry methods, and dried to 11% (wet basis) moisture content. Part of the beans was hulled, while the other part was hulled only after the beans were stored under two different environmental conditions: cooled air at 10 ºC with 50% relative humidity; and at 25 ºC without controlling the relative humidity. Samples were taken at 3, 6, and 12 months intervals in order to evaluate quality. The data were submitted to the simultaneous... |
| Tipo: Artigo de periódico |
Palavras-chave: Processing; Processamento; Coffea Arábica.; Hulling.. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065186 |
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