SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

SANTOS, M. de L.; ALVES, G. S. C.; COTTA, M. G.; FONSECA, F. C. A.; TOGAWA, R. C.; COSTA, M. M. do C.; ALVES, A. A.; MILLER, R. N. G.; SOUZA JUNIOR, M. T..

bitstream/item/171242/1/Resumo50CBFito-0637.pdf

A large-scale analysis of resistance gene analogs (RGAs) encoding NBS domains in the genus Elaeis.

Palavras-chave: E guineensis; E oleifera; NBS-LRRs; Biotic stress.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085646

SANTOS, M. de L.; SANTOS, M. de L.; COTTA, M. G.; FONSECA, F. C. A.; TOGAWA, R. C.; COSTA, M. M. do C.; ALVES, A. A.; MILLER, R. N. G.; SOUZA JUNIOR, M. T..

bitstream/item/173848/1/CBFITO-ePoster637.pdf

Aluminum-induced genes in grass species.

Palavras-chave: NBS-LRR; Elaeis guineenses; Elaeis oleífera; Biotic stress.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1088997

NODA, R. W.; GUIMARAES, C. T.; GOMES, E. A.; CARNEIRO, N. P.; LANA, U. G. de P.; MAGALHAES, J. V. de; COSTA, M. M. do C.; SILVA, F. R. D.; BRAMMER, S. P.; LÂNGARO, N. C.; CARVALHO, L. J. C. B.; BEVITORI, R.; PURCINO, A. A. C..

bitstream/item/25479/1/Aluminum-induced.pdf

Analyses of cupuassu (Theobroma grandiflorum) transcriptome during interaction with Moniliophthora perniciosa, the causal agent of Witches' Broom disease.

Palavras-chave: Tolerância a alumínio; Sequence analysis; Genetics; Aluminum; Genes.

Ano: 2010

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/873120

FALCAO, L. L.; WERNECK, J. O. S.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SILVA JUNIOR, O. B. da; ALVES, R. M.; ALBUQUERQUE, P. S. B.; MARCELLINO, L. H..

bitstream/item/188167/1/867.pdf

Analysis of the transcriptome in Aspergillus tamarii during enzymatic degradation of sugarcane bagasse.

Palavras-chave: Cupuaçu; Theobroma Grandiflorum; Doença; Doença de Planta; Vassoura de Bruxa.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1101187

MIDORIKAWA, G. E. O.; CORREA, C. L.; NORONHA, E. F.; FERREIRA FILHO, E. X.; TOGAWA, R. C.; COSTA, M. M. do C.; SILVA JUNIOR, O. B. da; GRYNBERG, P.; MILLER, R. N. G..

bitstream/item/183671/1/Midorikawa-et-al-2018-Frontiers-in-Bioengineering-and-Biotechnology.pdf

Brazilian coffee genome project: an EST-based genomic resource.

Palavras-chave: Carbohydrate-active enzymes; XlnR; Sugar transporters; ClrA; Aspergillus tamarii; Transcriptome; Lignocellulose; Bioethanol.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1096563

VIEIRA, L. G. E.; ANDRADE, A. C.; COLOMBO, C. A.; MORAES, A. H. de A.; METHA, A.; OLIVEIRA, A. C. de; LABATE, C. A.; MARINO, C. L.; MONTEIRO-VITORELLO, C. de B.; MONTE, D. C.; GIGLIOTI, E.; KIMURA, E. T.; ROMANO, E.; KURAMAE, E. E.; LEMOS, E. G. M.; ALMEIDA, E. R. P. de; JORGE, E. C.; ALBUQUERQUE, E. V. S.; SILVA, F. R. da; VINECKY, F.; SAWAZAKI, H. E.; DORRY, H. F. A.; CARRER, H.; ABREU, I. N.; BATISTA, J. A. N.; TEIXEIRA, J. B.; KITAJIMA, J. P.; XAVIER, K. G.; LIMA, L. M. de; CAMARGO, L. E. A. de; PEREIRA, L. F. P.; COUTINHO, L. L.; LEMOS, M. V. F.; ROMANO, M. R.; MACHADO, M. A.; COSTA, M. M. do C.; SÁ, M. F. G. de; GOLDMAN, M. H. S.; FERRO, M. I. T.; TINOCO, M. L. P.; OLIVEIRA, M. C.; VAN SLUYS, M-A.; SHIMIZU, M. M.; MALUF, M. P.; EIRA, M. T. S. da; GUERREIRO FILHO, O.; ARRUDA, P.; MAZZAFERA, P.; MARIANI, P. D. S. C.; OLIVEIRA, R. L. B. C. de; HARAKAVA, R.; BALBAO, S. F.; TSAI, S. M.; MAURO, S. M. Z. di; SANTOS, S. N.; SIQUEIRA, W. J.; COSTA, G. G. L.; FORMIGHIERI, E. F.; CARAZZOLLE, M. F.; PEREIRA, G. A. G..

bitstream/item/177955/1/ID-26742-1.pdf

Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4.

Palavras-chave: Projeto genoma; Brasil; Café.

Ano: 2006

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/187149

MILLER, R. N. G.; PASSOS, M. A. N.; MENEZES, N. N. P.; SOUZA JUNIOR, M. T.; COSTA, M. M. do C.; AZEVEDO, V. C. R.; AMORIM, E. P.; PAPPAS JUNIOR, G. J.; CIAMPI, A. Y..

Banana is a nutritionally important crop across tropical and sub-tropical countries in sub-Saharan Africa, Central and South America and Asia. Although cultivars have evolved from diploid, triploid and tetraploid wild Asian species of Musa acuminata (A genome) and Musa balbisiana (B genome), many of today's commercial cultivars are sterile triploids or diploids, with fruit developing via parthenocarpy. As a result of restricted genetic variation, improvement has been limited, resulting in a crop frequently lacking resistance to pests and disease. Considering the importance of molecular tools to facilitate development of disease resistant genotypes, the objectives of this study were to develop polymorphic microsatellite markers from BAC clone sequences for...

Combinação de abordagens de análises de novo e guiadas pelo genoma para explorar dados de RNA-Seq de sementes oleaginosas para anotação de vias de ácidos graxos.

Palavras-chave: Banana; Musa sp.; Varieties; Marcador molecular; Melhoramento genetico vegetal.

Ano: 2010

URL: http://www.alice.cnptia.embrapa.br/handle/doc/860025

Infoteca-e

LEMOS, V. N. S.; TOGAWA, R. C.; COSTA, M. M. do C.; RECH FILHO, E. L.; GRYNBERG, P..

Elaeis guineensis (dendê), Jatropha curcas (pinhão-manso) e Ricinus communis (mamona) produzem ácidos graxos que podem ser utilizados como fonte renovável na matriz energética, apresentando um grande potencial biotecnológico para as indústrias da área. O objetivo deste trabalho foi identificar transcritos relacionados com a síntese de ácidos graxos e inferir a sua presença em vias metabólicas. Para isso, o RNA total de sementes destas três espécies foi extraído e sequenciado. Os transcritomas foram montados utilizando abordagens de novo e guiados pelo genoma e filtrados com o programa Evidential Gene para a obtenção de resultados robustos. Uma base de dados contendo 527 sequências de 170 códigos de enzimas únicos de 12 vias de metabolismo de ácidos graxos...

Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)

Palavras-chave: Pinhão-manso; RNA-Seq; Vias metabólicas; Dendê; Mamona; Elaeis Guineensis; Jatropha Curcas; Ricinus Communis.

Ano: 2021

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1131672

Contrasting effects of wild Arachis dehydrin under abiotic and biotic stresses.

MOTA, A. P. Z.; OLIVEIRA, T. N.; VINSON, C. C.; WILLIAMS, T. C. R.; COSTA, M. M. do C.; ARAUJO, A. C. G. de; DANCHIN, E. G. J.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M..

bitstream/item/196886/1/fpls-10-00497.pdf

Differentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions.

Palavras-chave: Genome-wide; Arabidopsis; Drought; Freezing; Meloidogyne; Root-knot nematodes.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108633

MÜLLER, B. S. de F.; SAKAMOTO, T.; SILVEIRA, R. D. D.; ZAMBUSSI-CARVALHO, P. F.; PEREIRA, M.; PAPPAS JUNIOR, G. J.; COSTA, M. M. do C.; GUIMARÃES, C. M.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO-BRONDANI, R. P..

Drought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed...

Expressão diferencial de genes em Pleurotus pulmonarius associados a degradação enzimática e destoxificação da torta de pinhão-manso.

Palavras-chave: Feijão; Phaseolus vulgaris; Deficiência hídrica; Beans; Abiotic stress; Water stress.

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/994817

GOMES, T. G.; ALVES, G. S. C.; HADI, S. I. I. A.; COSTA, M. M. do C.; MENDONCA, S.; MILLER, R. N. G.; SIQUEIRA, F. G. de.

bitstream/item/184260/1/EnPI-2018-8.pdf

Expressed sequence-tag analysis of ovaries of Brachiaria brizantha reveals genes associated with the early steps of embryo sac differentiation of apomictic plants.

Palavras-chave: Transcritoma; NGS; Pré-tratamento biológico; Macrofungos; Jatropha Curcas.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097269

SILVEIRA, E. D.; GUIMARÃES, L. A.; DUSI, D. M. de A.; SILVA, F. R. da; MARTINS, N. F.; COSTA, M. M. do C.; ALVES-FERREIRA, M.; CARNEIRO, V. T. de C..

Abstract - In apomixis, asexual mode of plant reproduction through seeds, an unreduced megagametophyte is formed due to circumvented or altered meiosis. The embryo develops autonomously from the unreduced egg cell, independently of fertilization. Brachiaria is a genus of tropical forage grasses that reproduces sexually or by apomixis. A limited number of studies have reported the sequencing of apomixis-related genes and a few Brachiaria sequences have been deposited at genebank databases. This work shows sequencing and expression analyses of expressed sequence-tags (ESTs) of Brachiaria genus and points to transcripts from ovaries with preferential expression at megasporogenesis in apomictic plants. From the 11 differentially expressed sequences from...

First microsatellite markers developed from cupuassu ESTs: application in diversity analysis and cross-species transferability to cacao.

Palavras-chave: Forage grass; Apomixis; Plant reproduction.

Ano: 2012

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/938024

SANTOS, L. F. dos; FREGAPANI, R. M.; FALCAO, L. L.; TOGAWA, R. C.; COSTA, M. M. do C.; LOPES, U. V.; GRAMACHO, K. P.; ALVES, R. M.; MICHELI, F.; MARCELLINO, L. H..

The cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively....

Gene expression analysis in Musa acuminata during compatible interactions with Meloidogyne incognita.

Palavras-chave: Cupuaçu; Fruta tropical; Melhoramento genético; Cacau.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1042190

CASTAÑEDA, N. E. N.; ALVES, G. S. C.; ALMEIDA, R. M.; AMORIM, E. P.; FERREIRA, C. F.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; SANTOS, J. R. P.; CARES, J. E.; MILLER, R. N. G..

Background and Aims: Endoparasitic root-knot nematodes (RKNs) ( Meloidogyne spp.) cause considerable losses in banana ( Musa spp.), with Meloidogyne incognita a predominant species in Cavendish sub-group bananas. This study investigates the root transcriptome in Musa acuminata genotypes 4297-06 (AA) and Cavendish Grande Naine (CAV; AAA) during early compatible interactions with M. incognita . Methods: Roots were analysed by brightfield light microscopy over a 35 d period to examine nematode penetration and morphological cell transformation. RNA samples were extracted 3, 7 and 10 days after inoculation (DAI) with nematode J2 juveniles, and cDNA libraries were sequenced using lllumina HiSeq technology. Sequences were mapped to the M. acuminata ssp....

Spring is coming: genetic analyses of the bud break date locus reveal candidate genes from the cold perception pathway to dormancy release in apple (Malus x domestica Borkh.)

Palavras-chave: Musa acuminata; Meloidogyne incognita; Root-knot nematode; Biotic stress; Transcriptome; Monocotyledons.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1076414

MIOTTO, Y. E.; TESSELE, C.; CZERMAINSKI, A. B. C.; PORTO, D. D.; FALAVIGNA, V. da S.; SARTOR, T.; CATTANI, A. M.; DELATORRE, C. A.; ALENCAR, S. A. de; SILVA JUNIOR, O. B. da; TOGAWA, R. C.; COSTA, M. M. do C.; PAPPAS JÚNIOR, G. J.; GRYNBERG, P.; OLIVEIRA, P. R. D. de; KVITSCHAL, M. V.; DENARDI, F.; BUFFON, V.; REVERS, L. F..

Chilling requirement (CR) for bud dormancy completion determines the time of bud break in apple (Malus × domestica Borkh.). The molecular control of bud dormancy is highly heritable, suggesting a strong genetic control of the trait. An available Infinium II SNP platform for genotyping containing 8,788 single nucleotide polymorphic markers was employed, and linkage maps were constructed in a F1 cross from the low CR M13/91 and the moderate CR cv. Fred Hough. These maps were used to identify quantitative trait loci (QTL) for bud break date as a trait related to dormancy release. A major QTL for bud break was detected at the beginning of linkage group 9 (LG9). This QTL remained stable during seven seasons in two different growing sites. To increase mapping...

Systemic and sex-biased regulation of OBP expression under semiochemical stimuli.

Palavras-chave: Bud dormancy; Linkage mapping; MdoFLC; MdoICE1; MdoPRE1; Apples; Chilling requirement.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109789

PAULA, D. P.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; MARTINS, N. F.; ANDOW, D. A..

bitstream/item/177766/1/s41598-018-24297-z.pdf

Transcriptome profiling-based analysis of carbohydrate-active enzymes in Aspergillus terreus involved in plant Bbomass degradation.

Palavras-chave: OBP transcripts; Anthonomus grandis grandis.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1092003

CORRÊA, C. L.; MIDORIKAWA, G. E. O.; FERREIRA FILHO, E. X.; NORONHA, E. F.; ALVES, G. S. C.; TOGAWA, R. C.; SILVA JUNIOR, O. B.; COSTA, M. M. do C.; GRYNBERG, P.; MILLER, R. N. G..

bitstream/item/217530/1/fbioe-08-564527.pdf

Transcriptome-based identification of highly similar odorant-binding proteins among neotropical stink bugs and their egg parasitoid.

Palavras-chave: Lignocellulosic biomass; Biorefinery; Carbohydrate-active enzymes; Aspergillus terreus; Transcriptome.

Ano: 2020

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126336

FARIAS, L. R.; SCHIMMELPFENG, P. H. C.; TOGAWA, R. C.; COSTA, M. M. do C.; GRYNBERG, P.; MARTINS, N. F.; BORGES, M.; MORAES, M. C. B.; LAUMANN, R. A.; BÁO, S. N.; PAULA, D. P..

Olfaction plays a fundamental role in insect survival through resource location and intra and interspecific communications. We used RNA-Seq to analyze transcriptomes for odorant-binding proteins (OBPs) from major stink bug pest species in Brazil, Euschistus heros, Chinavia ubica, and Dichelops melacanthus, and from their egg parasitoid, Telenomus podisi. We identified 23 OBPs in E. heros, 25 OBPs in C. ubica, 9 OBPs in D. melacanthus, and 7 OBPs in T. podisi. The deduced amino acid sequences of the full-length OBPs had low intraspecific similarity, but very high similarity between two pairs of OBPs from E. heros and C. ubica (76.4 and 84.0%) and between two pairs of OBPs from the parasitoid and its preferred host E. heros (82.4 and 88.5%), confirmed by a...