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Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene. Repositório Alice
CURI, R.A.; CHARDULO, L.A.L.; SILVEIRA, A.C.; OLIVEIRA, H.N. de..
The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR?RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR?RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Calpastatin gene; Meat texture; PCR?RFLP; SNP; Gene da calpastatina; Textura da carne.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/126257
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Mitochondrial DNA of Nellore and European x Nellore crossing cattle of high performance Repositório Alice
CURI, R.A.; MOTA, L.S.L.S. de; SILVEIRA, A.C..
The objective of this work was to evaluate, through a polymorphism in the ND5 gene of the bovine mitochondrial DNA, the frequency of Bos taurus indicus mtDNA individuals in a sample of Nellore purebred origin animals (n = 69) and crossbred animals originated from crosses of European sires and Nellore purebred origin females (n = 275). Only 2.26% (8/354) of the animals presented Bos taurus indicus mtDNA. The high frequency of Bos taurus taurus mtDNA in these animals can be a consequence of selection, once the animals studied are originated from selected lineages of high performance for meat production.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Bos taurus indicus; Bos taurus taurus; Beef cattle; DNA polymorphism; Gado de corte; Polimorfismo de DNA.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122797
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