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In vitro evaluation of the cytotoxic and trypanocidal activities of Ampelozizyphus amazonicus (Rhamnaceae) BJMBR
Rosas,L.V.; Cordeiro,M.S.C.; Campos,F.R.; Nascimento,S.K.R.; Januário,A.H.; França,S.C.; Nomizo,A.; Toldo,M.P.A.; Albuquerque,S.; Pereira,P.S..
Ampelozizyphus amazonicus Ducke is a tree commonly found in the Amazon region and an extract of its stem bark is popularly used as an antimalarial and anti-inflammatory agent and as an antidote to snake venom. Ursolic acid; five lupane type triterpenes: betulin, betulinic acid, lupenone, 3ß-hydroxylup-20(29)-ene-27,28-dioic acid, and 2a,3ß-dihydroxylup-20(29)-ene-27,28-dioic acid, and three phytosteroids: stigmasterol, sitosterol and campesterol, have been isolated from stem extracts of A. amazonicus Ducke. Their structures were characterized by spectral data including COSY and HMQC. In an in vitro biological screening of the isolated compounds, 3ß-hydroxylup-20(29)-ene-27,28-dioic acid was cytotoxic against the SKBR-3 human adenocarcinoma cell line (1 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ampelozizyphus amazonicus; Rhamnaceae; Pentacyclic triterpenes; Cytotoxic activity; Trypanocidal activity.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500009
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Use of pcr-rflp of thefla a gene for detection and subtyping of Campylobacter jejuni strains Potentially related to Guillain-barré syndrome, isolated from humans and animals BJM
Scarcelli,E.; Piatti,R.M.; Harakava,R.; Miyashiro,S.; Campos,F.R.; Souza,M.C.A.; Cardoso,M.V.; Teixeira,S.R.; Genovez,M.E..
The objectives of the present study were the subtyping of Campylobacter jejuni subsp. jejuni strains obtained from humans and different animal species using PCR-RFLP, and the detection, by means of the same technique, of strains related to serotype PEN O19:LIO 7, the main C. jejuni serotype linked to Guillain-Barré Syndrome (GBS). Seventy C. jejuni strains isolated from human feces (n=33), primates (n=15), dogs (n=5), swine (n=2), bovines (n=1), abortion material from goats (n=2) and poultry carcasses (n=12), all collected in the state of São Paulo, were subtyped by means of PCR-RFLP of fla A gene, using restriction endonucleases Hae III, Afa I and Mbo I. Seven subtypes were observed when using the enzyme Hae III; eight when using Mbo I; and seven when...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; PCR-RFLP; Subtyping; Guillain-Barré Syndrome.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400029
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