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Registros recuperados: 39
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A new methodology for the improvement of diagnostic immunohistochemistry in canine veterinary pathology: automated system using human monoclonal and polyclonal antibodies Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Silva,P.; Rêma,A.; Gärtner,F.; Gobbi,H.; Tafuri,W.L.; Schmitt,F.C..
The authors describe their experience with an automated immunohistochemical system applied to canine tissue samples. Twenty human cellular markers specific monoclonal and polyclonal antibodies and two different antigen retrieval methods were used in normal and neoplastic breast tissue, as well as skin samples obtained from female dogs of pure and mixed breeds. The antibodies tested were the most frequently used in human and veterinary medicine studies, employed with diagnostic purposes in breast pathology, as well as in cancer research. Most of them may be used to study other normal and abnormal tissues and included cytokeratins, progesterone receptor, c-erbB2, p53, MIB-1, PCNA, EMA, vimentin, desmin, alpha-actin, S-100, pan-cadherin, and E-cadherin. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Immunohistochemistry; Automated system; Mammary gland.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000300010
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Analysis of EGFR and HER-2 expressions in ductal carcinomas in situ in canine mammary glands Arq. Bras. Med. Vet. Zootec.
Silva,I.L.D.; Dias,A.P.M.; Bertagnolli,A.C.; Cassali,G.D.; Ferreira,E..
Biomolecular evidence has shown that ductal carcinoma in situ(DCIS) may develop into invasive carcinoma of the canine mammary gland, and mutations in proto-oncogenes HER2 and EGFR; two members of the family of epidermal growth factor receptors, may be involved in this process. The purpose of this study was the characterization of the immunohistochemical expression of the EGFR and HER2 proteins in the process of neoplastic transformation, supposedly present in ductal carcinomas in situin canine mammary glands. Fifteen cases of DCIS were evaluated, with a higher expression of HER2 and EGFR being observed in low-grade carcinomas when compared with high-grade neoplasms, and with a high positive statistical correlation in the latter. Results suggest that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Neoplasm; Immunohistochemistry; EGFR; CerbB2.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300763
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Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases Arq. Bras. Med. Vet. Zootec.
Santos,F.G.A.; Vasconcelos,A.C.; Nunes,J.E.S.; Cassali,G.D.; Paixão,T.A.; Martins,A.S.; Silva,S.S.; Martins,R.F.; Moro,L..
Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Venereal tumor; Apoptosis; Cell death.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300013
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Assessment of cell proliferation and prognostic factors in canine mammary gland tumors Arq. Bras. Med. Vet. Zootec.
Dutra,A.P.; Azevedo Júnior,G.M.; Schmitt,F.C.; Cassali,G.D..
Three methods for the analysis of cell proliferation, mitotic index/10 high-power fields (10 HPF), mitotic index/four sets of 10 HPF (40 HPF), and MIB-1 index were evaluated in a series of canine mammary gland tumors, as well as the possible correlation between them. Fifty-six canine mammary gland tumors, including 23 benign and 33 malignant, were studied. In addition, the prognostic impact of mitotic index/10 HPF, and histological malignancy grade were evaluated in 17 malignant tumors, being seven ductal and 10 metaplastic carcinomas. The three methods used to evaluate cell proliferation were correlated with the prognostic impact of mitotic index/10 HPF and histological malignancy grade. The results showed a strong association between mitotic figure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine mammary tumors; MIB-1 index; Mitosis counting.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600015
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Association of nucleolar organizing regions and Ki-67 expression with recurrence rate of hair follicle tumor in dogs Arq. Bras. Med. Vet. Zootec.
Souza,P.C.; Ocarino,N.M.; Tavares,W.L.F.; Boeloni,J.N.; Cassali,G.D.; Serakides,R..
Mitotic index, nuclear diameter, number of nucleolar organizing regions, and Ki-67 expression, in hair follicle tumors of 82 dogs were evaluated. Tissue specimens were used to prepare sections for histological staining for number of nucleolar organizing region and immunohistochemical staining for Ki-67. Tumors were classified as trichoblastoma (n=32), benign trichoepithelioma (n=30), pilomatricoma (n=7), malignant trichoepithelioma (n=6), infundibular keratinizing acanthoma (n=5), and tricholemmoma (n=2). Head, dorsum, and limbs were the most affected sites. Malignant trichoepithelioma presented significantly higher mitotic index, number of nucleolar organizing regions and Ki-67 expression. Regarding benign neoplasms, trichoblastoma presented significantly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Neoplasm; AgNOR; Hair follicle; Ki-67.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000500006
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Cell proliferation markers in the transplanted canine transmissible venereal tumor Arq. Bras. Med. Vet. Zootec.
Santos,F.G.A.; Moro,L.; Cassali,G.D.; Paixão,T.A.; Campos,P.P.; Silva,S.S.; Vasconcelos,A.C..
Adult male mongrel dogs were subcutaneously transplanted with the canine transmissible venereal tumor (TVT) on the hypogastric region. Twelve specimens of tumors were collected, half during the proliferative phase and the other half during the regressive phase. Fragments of the tumor were fixed in 10% buffered formalin and routinely processed for light microscopy. Sections of 4µm were stained by Schorr or AgNOR or either immunostained for MIB1 (Ki67). Schorr stain, AgNOR and MIB1 showed an increased proliferative activity through mitotic index, nuclear argyrophilic protein stain and cycling tumoral cells in the growing tumors, respectively. All of the three cell proliferation markers were able to distinguish the TVT in both evolution phases. MIB1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Venereal tumors; MIB1; AgNOR.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600010
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c-erbB-2 expression and nuclear pleomorphism in canine mammary tumors BJMBR
Dutra,A.P.; Granja,N.V.M.; Schmitt,F.C.; Cassali,G.D..
The objective of the present investigation was to study the expression of c-erbB-2 and MIB-1 and try to associate them with morphological features of the cell such as nuclear pleomorphism, mitotic count and histological grade in a series of 70 canine mammary gland tumors, 22 of them benign and 48 malignant. Tumors were collected at the Veterinary Hospital of UFMG (Brazil) and the Veterinary Faculty of Porto University (Portugal). c-erbB-2 expression was determined according to the guidelines provided by the manufacturer of the HercepTest system and nuclear pleomorphism, mitotic count and histological grade according the Elston and Ellis grading system. The HercepTest is the FDA-approved in vitro diagnostic test marketed by Dako. It is a semi-quantitative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine mammary tumors; Nuclear pleomorphism; Breast cancer; C-erbB-2 expression; MIB-1 expression.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100013
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Clinical and pathological aspects of multicentric hemangiosarcoma in a Pinscher dog Arq. Bras. Med. Vet. Zootec.
Martins,B.D.C.; Torres,B.B.J.; Rodriguez,A.A.M.; Gamba,C.O.; Cassali,G.D.; Lavalle,G.E.; Martins,G.D.C.; Melo,E.G..
A 3-year-old female Pinscher dog was presented with a history of lameness and pain in hind limbs for one week which acutely progressed to non-ambulatory paraparesis. The dog had been apathetic and anorexic during the six days prior to presentation. The neurological findings were compatible with upper motor neuron signs to the left hind limb due to a spinal cord compressive mass on the twelfth thoracic vertebral body. On the other hand, signs of lower motor neurons to the right hind limb were due to a mass with a pathological fracture in the right proximal femur, compressing the sciatic nerve. A histopathological analysis of all organs revealedthe proliferation of endothelial cells showingin filtrative growth and organization into vascular structures with a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Neuropathy; Hemangiosarcoma; CD-31; Immunohistochemistry.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000200003
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Cystadenocarcinoma simulating hemangiosarcoma of the salivary gland in dog: case report Arq. Bras. Med. Vet. Zootec.
Gamba,C.O.; Damasceno,K.A.; Campos,C.B.; Ferreira,E.; Cassali,G.D..
Cystadenocarcinoma is regarded as a rare adenocarcinoma variant in animals. This work reports the case of an 8-year-old female Poodle dog with salivary gland cystadenocarcinoma with morphological characteristics similar to a hemangiosarcoma. Histopathological analysis showed a tumor mass with cystic formations containing a large amount of red blood cells. In order to distinguish these two entities, periodic acid Schiff (PAS) staining and immunohistochemical analysis were carried out with the use of cytokeratin AE1/AE3 (CK) and CD31-specific antibodies. Neoplastic cells were PAS-negative, CK-positive and CD31-negative confirming their epithelial origin. Based on the findings, the diagnosis of high grade cystadenocarcinoma was established.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bitch; Salivary gland; CD31; Neoplastic cells.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300006
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Cytological and histopathological aspects of lipomas in Bothrops moojeni Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Amaral,V.F.; Silva,A.E.; Macri,I.; Maciel,R..
O presente relato descreve os achados citológicos e histológicos de seis neoplasias benignas em cinco ofídios da espécie Bothrops moojeni mantidas em cativeiro por um período médio de 11,4 anos. Os animais apresentavam nódulos subcutâneos com localização variada desde o terço anterior até a porção mediana. Os exames citológico e histopatológico revelaram tratar-se de tumores lipomatosos benignos compatíveis com o subtipo lipoma fusocelular.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bothrops moojeni; Lifoma; Tumor; Neoplasm.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000600017
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Cytological diagnosis of a metastatic canine mammary tumor in pleural effusion Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Gärtner,F.; Vieira da Silva,M.J.; Schmitt,F.C..
Descrevem-se os achados citomorfológicos de um tumor maligno de mama em uma cadela Poodle de sete anos de idade, o qual foi observado inicialmente pelo exame citológico do derrame pleural. Comparam-se os aspectos citológicos do derrame pleural e punção aspirativa com agulha fina do tumor com aqueles descritos para o câncer de mama na espécie humana.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dog; Cytology; Pleural effusion; Mammary tumor.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400003
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DNA flow cytometry of canine mammary tumors: comparative aspects with human breast tumors Arq. Bras. Med. Vet. Zootec.
Cassali,G.D.; Salvador,A.; Freitas,C.; Dutra,A.P.; Schmitt,F.C..
Flow cytometric analysis of DNA content was performed on 28 samples of canine mammary tumors. Nine of them were benign and 19 were malignant. All benign tumors and 11 malignant tumors (57.9%) were diploid (P<0.05). Form the aneuploid tumors, five (26.3%) were hyperdiploid, one (5.3%) hypodiploid, one (5.3%) near triploid and one (5.3%) multiploid. The analysis of the expression of the markers PR and CD31 revealed a significant difference between diploid and aneuploid tumors (P<0.05). The immunoreactivity of PR was higher in diploid tumors, while the immunoreactivity of CD31 was stronger in aneuploid tumors. No difference between the markers MIB-1, c-erbB2, p53 and Cyclin D1 was observed (P>0.05). Using the flow cytometry analysis and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dog; Breast cancer; Neoplasm; DNA flow cytometry; Immunohistochemistry.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500011
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Double-staining immunohistochemistry of canine mammary neoplasms Arq. Bras. Med. Vet. Zootec.
Pereira,F.M.; Ferreira,E.; Leitão,D.R.A.; Cassali,G.D..
Descreve-se a técnica de dupla-marcação em imunoistoquímica aplicada a tumores mamários em cadelas incluídos em parafina. Foram utilizados dois tipos de neoplasia em que ocorre envolvimento de células epiteliais, mioepiteliais e mesenquimais, tumor misto benigno e carcinossarcoma. Foram testados dois anticorpos, AE1/AE3 para identificação de tecido epitelial e S100 como marcador de células mioepiteliais. A dupla marcarcação em imunoistoquímica pode ser utilizada como ferramenta importante na pesquisa e diagnóstico de lesões mamárias complexas.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cão; Glândula mamária; Imunoistoquímica; Neoplasia.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400037
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Efeito do miriadenolídeo isolado de Alomia myriadenia (Asteraceae) sobre o tumor de Erlich ascítico no camundongo Arq. Bras. Med. Vet. Zootec.
Verçosa Júnior,D.; Souza-Fagundes,E.M.; Cassali,G.D.; Ribeiro,E.L.; Zani,C.L.; Melo,M.M..
Estudou-se atividade antineoplásica de um produto natural isolado de Alomia myriadenia (miriadenolídeo) no modelo do tumor de Ehrlich em camundongos. Dezoito fêmeas de camundongo Swiss foram inoculadas com 2x10(7) células viáveis de tumor de Ehrlich via intraperitoneal (0,3ml) e posteriormente distribuídas aleatoriamente em três grupos que receberam: grupo I (controle) - 0,3ml de solução de Hanks; grupo II - 31µg/kg de miriadenolídeo; e grupo III - 139µg/kg de miriadenolídeo. No oitavo dia de experimento, foram realizados exames hematológicos e perfil protéico sérico eletroforético. Coletou-se todo o líquido ascítico para avaliação do volume, aparência, pH, contagem de células viáveis e inviáveis, realização de esfregaços para contagem de células claras e...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Miriadenolídeo; Tumor de Ehrlich; Hematologia; Eletroforese; Alterações microscópicas; Camundongos.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500014
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Effects of different extracts of the mushroom Agaricus blazei Murill on the hematologic profile of mice with Ehrlich tumor Arq. Bras. Med. Vet. Zootec.
Verçosa Júnior,D.; Ferraz,V.P.; Duarte,E.R.; Oliveira,N.J. F.; Soto-Blanco,B.; Cassali,G.D.; Melo,M.M..
The aim of this study was to investigate the effect of the mushroom Agaricus blazeii Murril (ABM) extracts on the hematological profile of Swiss mice bearing an Ehrlich solid tumor. Three fractions (total extract, polysaccharides, and supernatant) of ABM extracts obtained by four methods (ultrasonic or water bath, at pH 4 or pH 7) were administered to mice over 21 days. Polysaccharide solutions were analyzed by gas and liquid chromatography that showed both mannose and glucose concentrations. The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. The treatment with ABM supernatant at pH 7 and water bath was associated with reduced concentrations of leukocytes and lymphocytes and altered the percentage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mouse; Ehrlich solid tumor; Polysaccharides; Hematological profile.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300679
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Epidemiological, clinical and pathological evaluation of overall survival in canines with mammary neoplasms Arq. Bras. Med. Vet. Zootec.
Nunes,F.C.; Campos,C.B.; Teixeira,S.V.; Bertagnolli,A.C.; Lavalle,G.E.; Cassali,G.D..
ABSTRACT Canine mammary neoplasms (CMNs) are the most frequent lesions and in female dogs. However, studies correlating pathological criteria with clinical evolution in female dogs with mammary neoplasms are scarce. The present study aims to present epidemiological, clinical-pathological and overall survival data to help establish the prognosis and understand the biological behavior of CMNs. A total of 1539 cases were included (85% malignant and 13% benign). Tumor size was an important prognostic factor and was associated with overall patient survival (P< 0.0001). Most dogs diagnosed with malignant neoplasms (83%) had initial clinical staging, although 17% had regional or distant metastases at the time of diagnosis and lower overall survival (P<...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Female dogs; Mammary gland; Carcinomas; Prognosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601714
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Evaluation of 99mTc-HYNIC-βAla-Bombesin(7-14) as an agent for pancreas tumor detection in mice BJMBR
Carlesso,F.N.; Fuscaldi,L.L.; Araújo,R.S.; Teixeira,C.S.; Oliveira,M.C.; Fernandes,S.O.A.; Cassali,G.D.; Reis,D.C.; Barros,A.L.B.; Cardoso,V.N..
Pancreatic adenocarcinoma is important in oncology because of its high mortality rate. Deaths may be avoided if an early diagnosis could be achieved. Several types of tumors overexpress gastrin-releasing peptide receptors (GRPr), including pancreatic cancer cells. Thus, a radiolabeled peptide derivative of gastrin-releasing peptide (GRP) may be useful as a specific imaging probe. The purpose of the present study was to evaluate the feasibility of using99mTc-HYNIC-βAla-Bombesin(7-14)as an imaging probe for Capan-1 pancreatic adenocarcinoma. Xenographic pancreatic tumor was developed in nude mice and characterized by histopathological analysis. Biodistribution studies and scintigraphic images were carried out in tumor-bearing nude mice. The two methods...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 99mTc-HYNIC-βAla-Bombesin; Capan-1; Scintigraphic images; Pancreas tumor; Biodistribution.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000010
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First description of basaloid carcinoma of the canine mammary gland: case report Arq. Bras. Med. Vet. Zootec.
Nakagaki,K.Y.R.; Gonçalves,A.B.B.; Rocha,R.M.; Cassali,G.D..
ABSTRACT The objective of this case report was to describe histopathological and immunohistochemical characteristics of the first reported basaloid carcinomas in the canine mammary gland. Two bitches were treated for tumors in the mammary gland and underwent mastectomy. Microscopic evaluation of these tumors revealed epithelial cells arranged in a predominantly solid pattern with hyperchromatic peripheral cells arranged in a palisade pattern. Metastases in regional lymph nodes were found in both animals, and one bitch exhibited pulmonary metastasis. Immunohistochemistry revealed positive labeling for the basal cell markers cytokeratin 14 and p63. Histopathological and immunohistochemical findings led to diagnoses of basaloid carcinoma of the canine mammary...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mammary gland; Salivary gland; Basaloid carcinoma; Basaloid adenoma; Solid carcinoma.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300878
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Glycogen-rich clear cell carcinoma of the feline mammary gland: case report Arq. Bras. Med. Vet. Zootec.
Campos,C.B.; Gamba,C.O.; Damasceno,K.A.; Lavalle,G.E.; Cassali,G.D..
ABSTRACT The aim of this report is to describe the first histopathological, immunohistochemical, and clinical characteristics of a feline glycogen-rich clear cell carcinoma (GRCCC). A Persian queen was admitted with mammary gland tumors and underwent radical unilateral mastectomy. Overall survival was considered 33 days and death was due to clinical evolution of the disease. Microscopic evaluation demonstrated epithelial cells arranged in a predominantly solid pattern, tumor cells presented an ample, granular, and foamy clear cytoplasm, and moderate cellular pleomorfism. The presence of cytoplasmatic glycogen was confirmed through diastase digestion followed by PAS staining. Histopathological and histochemical findings lead to the diagnosis of GRCCC with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Feline; Mammary gland; Neoplasm; Glycogen-rich clear cell carcinoma.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501117
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Gross and histologic features of gastritis due to Ophidascaris arndti in tropical rattlesnakes ( Crotalus durissus ) Arq. Bras. Med. Vet. Zootec.
Mello,É.M.; Salgado,B.S.; Cassali,G.D.; Guimarães,M.P..
ABSTRACT The tropical rattlesnake (Crotalus durissus) is a snake of great importance for biomedical industry since its poison is used for the production of antiophidic serum and researches. Several conditions related to animal health, such as diseases and parasites, which can promote the reduction of poison production by these snakes should be investigated. Accordingly, the aim of this study was to characterize the gross and microscopic lesions related to the presence of Ophidascaris arndti in stomachs of tropical rattlesnakes. The gastrointestinal tract of thirty-five South American rattlesnakes captured in Southeastern region of Brazil were analyzed and nineteen animals showed infestation by the parasites, found in the small intestine and, especially, in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snake; Parasitic granuloma; Parasitic gastritis; Nematoda.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200415
Registros recuperados: 39
Primeira ... 12 ... Última
 

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