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| Langier,S.; Galvani,R.G.; Alves,A.P.G.; Fidelis,R.; Nunes,P.H.C.; Silva,M.H.; Castilho,L.R.; Monteiro,J.P.; Bonomo,A.. |
| The participation of regulatory T (Treg) cells in B cell-induced T cell tolerance has been claimed in different models. In skin grafts, naive B cells were shown to induce graft tolerance. However, neither the contribution of Treg cells to B cell-induced skin tolerance nor their contribution to the histopathological diagnosis of graft acceptance has been addressed. Here, using male C57BL/6 naive B cells to tolerize female animals, we show that skin graft tolerance is dependent on CD25+ Treg cell activity and independent of B cell-derived IL-10. In fact, B cells from IL-10-deficient mice were able to induce skin graft tolerance while Treg depletion of the host inhibited 100% graft survival. We questioned how Treg cell-mediated tolerance would impact on... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Skin graft; Tolerance; B cells; Treg; Histopathology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000009 |
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| Fernandes,A.M.; Marinho,P.A.N.; Sartore,R.C.; Paulsen,B.S.; Mariante,R.M.; Castilho,L.R.; Rehen,S.K.. |
| Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up hES cell production. Maintenance of pluripotency in the microcarrier-based stirred system was shown by immunocytochemical and flow cytometry analyses for pluripotency-associated markers. The formation of cavitated embryoid bodies expressing markers of endoderm, ectoderm and mesoderm was further evidence of maintenance of differentiation capability. Cell yield per volume of medium spent was more than... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human embryonic stem cells; Scale-up; Microcarriers; Spinner bioreactors. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600007 |
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