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A micromethod for high throughput RNA extraction in forest trees Inra
Le Provost, G.; Herrera, R.; Paiva, J.A.; Chaumeil, P.; Salin, F.; Plomion, C..
Tipo: Journal Article Palavras-chave: MICROMETHODE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20088d667a03&uri=/notices/prodinra1/2008/05/
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Interaction between environmental factors affects the accumulation of root proteins in hydroponically grown Eucalyptus globulus (Labill.) Inra
Bedon, F.; Majada, J.; Feito, I.; Chaumeil, P.; Dupuy, J.-W.; Lomenech, A.-M.; Barre, A.; Gion, J.M.; Plomion, C..
Eucalyptus globulus (Labill.) is used for pulp and paper production worldwide. In this report we studied changes in protein expression in one osmotically stressed elite clone widely used in industrial plantations in Spain. High molecular weight polyethylene glycol (PEG) was used as an osmoticum in the growing medium. Roots of rooted cuttings were sampled after 3 and 36 h of treatment. Water potential and abscissic acid content were measured in shoot and root apices to characterize the physiological states of the plants. Total soluble proteins from roots were extracted and separated using two-dimensional gel electrophoresis (2-DE). Gels were stained with Coomassie brillant blue for quantitative analysis of protein accumulation. From a total of 406...
Tipo: Journal Article Palavras-chave: STRESS OSMOTIQUE; EUCALYPTUS GLOBULUS.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20111c4663c8&uri=/notices/prodinra1/2011/04/
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