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| Tu,Zhiming; He,Guangyuan; Li,Kexiu X.; Chen,Mingjie J.; Chang,Junli; Chen,Ling; Yao,Qing; Liu,Dongping P.; Ye,Huan; Shi,Jiantao; Wu,Xuqian . |
| This paper describes an efficient bacterial transformation system that was established for the preparation of competent cells, plasmid preparation, and for the storage in bacterial stocks in our laboratory. Using this method, a number of different plasmids have been amplified for further experiments. Competent cells for bacterial transformation were prepared by the calcium chloride method with an optimum concentration of 75 mM. Three different strains of Escherichia coli that were tested are DH5α, TG1 and XL1 blue, and the most efficient strain being XL1 blue. The optimal optical density (OD600) range for competent cell preparation varied for each of the strains investigated, and for XL1 blue it was 0.15-0.45; for TG1 it was 0.2-0.5; and for... |
| Tipo: Journal article |
Palavras-chave: Competent cells; E. coli; Plasmid; Storage; Transformation. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100014 |
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| Jiang,Lai; Tang,Chaoliang; Gong,Yanping; Liu,Yujie; Rao,Jie; Chen,Suyu; Qu,Wanjun; Wu,Dabao; Lei,Lei; Chen,Ling. |
| Pregnancy-induced hypertension (PIH) causes significant maternal and fetal morbidity and mortality. A decreased number of regulatory T (Treg) cells is associated with the pathogenesis of PIH. The programmed cell death-1 (PD-1)/PD-ligand 1 (PD-L1) pathway is critical to normal pregnancy (NP) by promoting Treg cell development. However, the relationship between PD-1/PD-L1 and Treg differentiation in PIH has not been fully elucidated. In this study, venous blood was obtained from 20 NP and 58 PIH patients. Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood. The levels of Treg-related cytokines (TGF-β, IL-10, and IL-35) in serum and PBMCs were measured by ELISA. The percentage of Treg cells in PBMCs was assessed by flow cytometry. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PD-1/PD-L1; Treg; PIH; Foxp3; Differentiation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800605 |
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