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Campylobacter Jejuni Increases Transcribed Il-1 B and Causes Morphometric Changes in the Ileal Enterocytes of Chickens Rev. Bras. Ciênc. Avic.
Fonseca,BB; Ferreira Júnior,A; Santos,JP dos; Coelho,LR; Rossi,DA; Melo,RT; Mendonça,EP; Araújo,TG; Alves,RN; Beletti,ME.
Abstract Campylobacteriosis is a worldwide foodborne zoonosis disease caused by Campylobacter jejuni. This microorganism is considered a commensal bacterium in chicken hosts. C. jejuni produces epithelial cell modifications and induces a cytokine gene transcription innate immunity repertoire. In the present study, we describe the invasiveness, morphological cellular modifications, and transcript level expressions of innate immune cytokines from C. jejuni-inoculated chicken ileum explants. C. jejuni was internalized by epithelial ileum cells at 15 minutes postinoculation (p.i.) and was detected intracellularly for 4hs (p.i.). Inoculated explants displayed significant increases in cell height. C. jejuni induced a significant elevation of Transforming Growth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Campylobacter jejuni; Chicken; Explants; TGF-b3; IL-1b.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100063
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Effects of inverting the position of layers eggs during storage on hatchery performance parameters Rev. Bras. Ciênc. Avic.
Lima,JCS de; Silva,PL; Coelho,LR; Borges,MS; Freitas,AG de; Fonseca,BB.
Storing hatchable eggs is a common practice in commercial hatcheries. However, storage time may negative effects on several performance parameters. An experiment was carried out to evaluate inverting egg position during storage of eggs laid by young and old layer breeders. Fertile eggs of 32 and 58-week-old breeders were stored for seven, 14, and 21 days at 18ºC ± 2ºC and 80% ± 10% relative humidity (RU). The following parameters were evaluated: egg weight loss, hatchability and hatchling weight, and embryodiagnosis results. Eggs stored with the small end up lost less weight during storage compared with the control eggs. Storing eggs for 14 days with the small end up reduced early embryo mortality, improving hatchability. In addition, hatchling weight...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Storage; Layer breeders; Hatchling weight; Egg position; Hatchery performance.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000400003
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Validation of Alternative Methods of Detection of Salmonella Spp. in Experimentally Contaminated Poultry Environmental Samples Rev. Bras. Ciênc. Avic.
Coelho,LR; Melo,RT; Monteiro,GP; Reischak,D; Mendonça,AO; Tavares,AAS; Rossi,DA.
ABSTRACT The analysis of Salmonella in the feces and the birds’ environment is a way of monitoring the colonization in the flocks and verifying the need for the introduction of stricter controls, in such a way that the results of the tests should be known before being sent for slaughter. The polymerase chain reaction (PCR), as well as other rapid methods represent alternatives increasingly used to detect enteric pathogens, but they need proof of effectiveness for their wide use. The aim of this study was to evaluate the equivalence between the results obtained by the methods: real-time PCR (BAX® System), Modified Rappaport-Vassiliadis Semi-solid Medium (MSRV) (ISO 6579) and the traditional method of official reference in Brazil for research of S....
Tipo: Info:eu-repo/semantics/article Palavras-chave: BAX® System; MSRV; Salmonella Enteritidis; S. Typhimurium; Molecular biology.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200303
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