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Registros recuperados: 5
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A biolistic process for in vitro gene transfer into chicken embryos BJMBR
Ribeiro,L.A.; Mariani,P.D.S.C.; Azevedo,J.L.; Rech,E.L.; Schmidt,G.S.; Coutinho,L.L..
Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100%, survival rate 25% and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biolistic process; Chicken embryo; Gene transfer; SS-galactosidase; Green fluorescent protein; GFP.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900003
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Influence of the neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos BJMBR
Alves,H.J.; Alvares,L.E.; Gabriel,J.E.; Coutinho,L.L..
Important advances have been made in understanding the genetic processes that control skeletal muscle formation. Studies conducted on quails detected a delay in the myogenic program of animals selected for high growth rates. These studies have led to the hypothesis that a delay in myogenesis would allow somitic cells to proliferate longer and consequently increase the number of embryonic myoblasts. To test this hypothesis, recently segmented somites and part of the unsegmented paraxial mesoderm were separated from the neural tube/notochord complex in HH12 chicken embryos. In situ hybridization and competitive RT-PCR revealed that MyoD transcripts, which are responsible for myoblast determination, were absent in somites separated from neural tube/notochord...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken development; MyoD; Myogenesis; In situ hybridization; BrdU; Cellular proliferation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200005
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Selection for breed-specific growth hormone and IGF-I alleles in a synthetic beef cattle cross, Canchim Genet. Mol. Biol.
Regitano,L.C.A.; Azevedo,J.L.; Vencovsky,R.; Packer,I.U.; Barbosa,P.F.; Rosa,A.J.M.; Silva,N.A.; Etchegaray,M.A.L.; Coutinho,L.L..
This study was developed to evaluate selection effects on gene frequencies in a synthetic beef cattle cross (5/8 Charolais, 3/8 Zebu) named Canchim. A sample of 154 Canchim animals, representing three generation classes, was analyzed for seven molecular markers. Thirty-six Charolais cattle were also studied for comparison. A highly significant variation (P < 0.01), with a linear increase of the allele coding for valine at position 127 of the growth hormone peptide, was observed through generations. This allele was found in the Charolais sample but is not observed in Brazilian Bos indicus breeds. Four alleles were found for a microsatellite in the 5' flanking region of insulin-like growth factor I gene in Canchim. Allele sizes ranged from 231 to 225...
Tipo: Info:eu-repo/semantics/article
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000400011
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Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos BJMBR
Alvares,L.E.; Mantoani,A.; Corrente,J.E.; Coutinho,L.L..
The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present study was to develop and standardize a competitive PCR method using standard-curves to quantify transcripts of the myogenic regulatory factors MyoD, Myf-5, Myogenin and MRF4 in chicken embryos. Competitor cDNA molecules were constructed for each gene under study using deletion primers, which were designed to maintain the anchorage sites for the primers used to amplify target cDNAs. Standard-curves were prepared by co-amplification of different amounts of target cDNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quantitative RT-PCR; Standard-curve; Myogenic regulatory factors; Gene expression.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200004
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Temporal expression pattern of myostatin transcripts during chicken embryogenesis Arq. Bras. Med. Vet. Zootec.
Gabriel,J.E.; Alvares,L.E.; Paz,C.C.P.; Packer,I.U.; Coutinho,L.L..
No presente estudo, estimou-se a abundância dos transcritos da miostatina foi estimada durante a embriogênese de galinha por análises de RT-PCR competitiva. Níveis basais de mRNA desse gene foram detectados até o estádio HH15, enquanto acúmulos significativos nesses níveis foram observados apenas no estádio HH24, seguido por redução na abundância desses transcritos a partir do estádio HH26. Tais descobertas preliminares proporcionam informações relevantes sobre a ativação do fator de crescimento miostatina durante o desenvolvimento in ovo de aves.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Chicken embryo; Myostatin; Temporal expression; MRNA abundance; Competitive RT-PCR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500033
Registros recuperados: 5
Primeira ... 1 ... Última
 

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