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Registros recuperados: 77
Primeira ... 1234 ... Última
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A case for re-inventory of Australia’s plant pathogens Naturalis
Hyde, K.D.; Chomnunti, P.; Crous, P.W.; Groenewald, J.Z.; Damm, U.; Ko Ko, T.W.; Shivas, R.G.; Summerell, B.A.; Tan, Y.P..
Several filamentous oomycete species of the genus Halophytophthora have recently been described from marine environments, mostly from subtropical and tropical ecosystems. During a survey of oomycetes from leaf litter of Spartina alterniflora in salt marshes of southeastern Georgia, isolates of four taxa were recovered that bore similarity to some members of Halophytophthora but were highly divergent from isolates of Halophytophthora s.str. based on a combined sequence analysis of two nuclear loci. In phylogenetic analyses, these isolates were placed basal to a monophyletic group comprised of Pythium of the Pythiaceae and the Peronosporaceae. Sequence and morphology of these taxa diverged from the type species Halophytophthora vesicula, which was placed...
Tipo: Article / Letter to the editor Palavras-chave: Internal transcribed spacer; Nuclear ribosomal large subunit (nrLSU); Peronosporales; Phylogeny; Pythiaceae.
Ano: 2010 URL: http://www.repository.naturalis.nl/record/532294
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A fissitunicate ascus mechanism in the Calosphaeriaceae, and novel species of Jattaea and Calosphaeria on Prunus wood Naturalis
Damm, U.; Crous, P.W.; Fourie, P.H..
During a survey of Prunus wood from South Africa, isolations were made of three presumably Calosphaerialean fungi that formed hyphomycetous, phialidic anamorphs in culture. In order to reveal the phylogenetic relationship of these fungi, they were characterised on a morphological and molecular (LSU and ITS rDNA) basis. Two isolates that formed a teleomorph in culture are newly described as Calosphaeria africana sp. nov. Although asci of Calosphaeria are characterised by having non-amyloid apical rings, two functional wall layers were observed in asci of C. africana, which has hitherto not been observed in any member of the Calosphaeriaceae. However, Calosphaeriaceae (Calosphaeriales, Sordariomycetes) are not closely related to other bitunicate fungi like...
Tipo: Article / Letter to the editor Palavras-chave: Apical ring; Ascospore discharge; Ascus dehiscence; Beta-tubulin; Calosphaeria; Calosphaeriaceae; ITS; Jattaea; LSU; Phialophora; Prunus; SSU; Systematics.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/531626
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A re-appraisal of Harknessia (Diaporthales), and the introduction of Harknessiaceae fam. nov. Naturalis
Crous, P.W.; Summerell, B.A.; Shivas, R.G.; Carnegie, A.J.; Groenewald, J.Z..
Harknessiaceae is introduced as a new family in the ascomycete order Diaporthales to accommodate species of Harknessia with their Wuestneia-like teleomorphs. The family is distinguished by having pycnidial conidiomata with brown, furfuraceous margins, brown conidia with hyaline, tube-like basal appendages, longitudinal striations, and rhexolytic secession. Six species occurring on Eucalyptus are newly introduced, namely H. australiensis, H. ellipsoidea, H. pseudohawaiiensis, and H. ravenstreetina from Australia, H. kleinzeeina from South Africa, and H. viterboensis from Italy. Epitypes are designated for H. spermatoidea and H. weresubiae, both also occurring on Eucalyptus. Members of Harknessia are commonly associated with leaf spots, but also occur as...
Tipo: Article / Letter to the editor Palavras-chave: Biodiversity; Fungal pathogens; Harknessiaceae; ITS; LSU; Phylogeny; Systematics.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/532250
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Application of the consolidated species concept to Cercospora spp. from Iran Naturalis
Bakhshi, M.; Arzanlou, M.; Babai-ahari, A.; Groenewald, J.Z.; Braun, U.; Crous, P.W..
The genus Cercospora includes many important plant pathogenic fungi associated with leaf spot diseases on a wide range of hosts. The mainland of Iran covers various climatic regions with a great biodiversity of vascular plants, and a correspondingly high diversity of cercosporoid fungi. However, most of the cercosporoid species found to date have been identified on the basis of morphological characteristics and there are no cultures that support these identifications. In this study the Consolidated Species Concept was applied to differentiate Cercospora species collected from Iran. A total of 161 Cercospora isolates recovered from 74 host species in northern Iran were studied by molecular phylogenetic analysis. Our results revealed a rich diversity of...
Tipo: Article / Letter to the editor Palavras-chave: Biodiversity; Cercospora apii complex; Cercosporoid; Host specificity; Leaf spot; Multilocus sequence typing (MLST); Mycosphaerella; Taxonomy.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/560054
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Calonectria (Cylindrocladium) species associated with dying Pinus cuttings Naturalis
Lombard, L.; Rodas, C.A.; Crous, P.W.; Wingfield, B.D.; Wingfield, M.J..
Calonectria (Ca.) species and their Cylindrocladium (Cy.) anamorphs are well-known pathogens of forest nursery plants in subtropical and tropical areas of the world. An investigation of the mortality of rooted Pinus cuttings in a commercial forest nursery in Colombia led to the isolation of two Cylindrocladium anamorphs of Calonectria species. The aim of this study was to identify these species using DNA sequence data and morphological comparisons. Two species were identified, namely one undescribed species, and Cy. gracile, which is allocated to Calonectria as Ca. brassicae. The new species, Ca. brachiatica, resides in the Ca. brassicae species complex. Pathogenicity tests with Ca. brachiatica and Ca. brassicae showed that both are able to cause disease...
Tipo: Article / Letter to the editor Palavras-chave: Β-tubulin; Calonectria; Cylindrocladium; Histone; Pinus; Root disease.
Ano: 2009 URL: http://www.repository.naturalis.nl/record/532052
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Calonectria species associated with cutting rot of Eucalyptus Naturalis
Lombard, L.; Zhou, X.D.; Crous, P.W.; Wingfield, B.D.; Wingfield, M.J..
Decline in the productivity of Eucalyptus hybrid cutting production in the Guangdong Province of China is linked to cutting rot associated with several Calonectria spp. The aim of this study was to identify these fungi using morphological and DNA sequence comparisons. Two previously undescribed Calonectria spp., Ca. pseudoreteaudii sp. nov. and Ca. cerciana sp. nov. were identified together with Ca. pauciramosa. Calonectria pseudoreteaudii resides in the Ca. reteaudii complex and Ca. cerciana is closely related to Ca. morganii. Connected to the discovery of Ca. pseudoreteaudii, species in the Ca. reteaudii complex were re-considered and the group is shown to accommodate two cryptic species. These originate from Australia and are described as Ca....
Tipo: Article / Letter to the editor Palavras-chave: Australia; Calonectria; China; Cylindrocladium; Eucalyptus; Systematics.
Ano: 2010 URL: http://www.repository.naturalis.nl/record/531722
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Calonectria spp. causing leaf spot, crown and root rot of ornamental plants in Tunisia Naturalis
Lombard, L.; Polizzi, G.; Guarnaccia, V.; Vitale, A.; Crous, P.W..
Calonectria spp. are important pathogens of ornamental plants in nurseries, especially in the Northern Hemisphere. They are commonly associated with a wide range of disease symptoms of roots, leaves and shoots. During a recent survey in Tunisia, a number of Calonectria spp. were isolated from tissues of ornamental plants showing symptoms of leaf spot, crown and root rot. The aim of this study was to identify these Calonectria spp. using morphological and DNA sequence comparisons. Two previously undescribed Calonectria spp., C. pseudomexicana sp. nov. and C. tunisiana sp. nov., were recognised. Calonectria mexicana and C. polizzii are newly reported for the African continent. Pathogenicity tests with all four Calonectria spp. showed that they are able to...
Tipo: Article / Letter to the editor Palavras-chave: Calonectria; Crown and root rot; DNA phylogeny; Leaf spot; Pathogenicity; Systematics.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/531828
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Caulicolous Botryosphaeriales from Thailand Naturalis
Trakunyingcharoen, T.; Lombard, L.; Groenewald, J.Z.; Cheewangkoon, R.; To-anun, C.; Crous, P.W..
Members of Botryosphaeriales are commonly encountered as endophytes or pathogens of various plant hosts. The Botryosphaeriaceae represents the predominant family within this order, containing numerous species associated with canker and dieback disease on a wide range of woody hosts. During the course of routine surveys from various plant hosts in Thailand, numerous isolates of Botryosphaeriaceae, including Aplosporellaceae were collected. Isolates were subsequently identified based on a combination of morphological characteristics and phylogenetic analysis of a combined dataset of the ITS and EF1-α gene regions. The resulting phylogenetic tree revealed 11 well-supported clades, correlating with different members of Botryosphaeriales. Other than confirming...
Tipo: Article / Letter to the editor Palavras-chave: Aplosporella; Botryosphaeriaceae; Diplodia; Lasiodiplodia; Multigene phylogeny; Pseudofusicoccum; Sexual morph; Systematics.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/560051
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Coniochaeta (Lecythophora), Collophora gen. nov. And Phaeomoniella species associated with wood necroses of Prunus trees Naturalis
Damm, U.; Fourie, P.H.; Crous, P.W..
Species of the genus Coniochaeta (anamorph: Lecythophora) are known as pathogens of woody hosts, but can also cause opportunistic human infections. Several fungi with conidial stages resembling Lecythophora were isolated from necrotic wood samples of Prunus trees in South Africa. In order to reveal their phylogenetic relationships, these fungi were studied on a morphological and molecular (5.8S nrDNA, ITS-1, ITS-2, GAPDH, EF-1α, 28S nrDNA, 18S nrDNA) basis. Some of the isolates were identified as Coniochaeta (Sordariomycetes), including C. velutina and two new species, C. africana and C. prunicola. The majority of the isolates, however, formed pycnidial or pseudopycnidial synanamorphs and were not closely related to Coniochaeta. According to their 28S...
Tipo: Article / Letter to the editor Palavras-chave: Collophora; Coniochaeta; EF-1α; GAPDH; ITS; Lecythophora; LSU; Pathogenicity; Phaeomoniella; Prunus; SSU; Systematics.
Ano: 2010 URL: http://www.repository.naturalis.nl/record/531834
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Co-occurring species of Teratosphaeria on Eucalyptus Naturalis
Crous, P.W.; Groenewald, J.Z.; Summerell, B.A.; Wingfield, B.D.; Wingfield, M.J..
A common leaf spot disease occurring on Eucalyptus cladocalyx and E. lehmannii in the Western Cape Province of South Africa is known from literature to be caused by the fungus Coniothyrium ovatum, which is a pathogen native to several eucalypts in Australia. Recent collections have shown that Australian material identified as C. ovatum is morphologically and phylogenetically distinct from the South African specimens, and that all these taxa would be better accommodated in the genus Teratosphaeria. South African specimens previously identified as C. ovatum were found to represent two species that co-occur in the same leaves and even spots and are described here as T. juvenalis and T. verrucosa. Furthermore, a fresh collection of T. ovata from E. phoenicea...
Tipo: Article / Letter to the editor Palavras-chave: Colletogloeopsis; Coniothyrium; Eucalyptus; Kirramyces; Mycosphaerella; Mycosphaerella leaf disease; Readeriella; Taxonomy.
Ano: 2009 URL: http://www.repository.naturalis.nl/record/532187
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Cymadothea trifolii, an obligate biotrophic leaf parasite of Trifolium, belongs to Mycosphaerellaceae as shown by nuclear ribosomal DNA analyses Naturalis
Simon, U.K.; Groenewald, J.Z.; Crous, P.W..
The ascomycete Cymadothea trifolii, a member of the Dothideomycetes, is unique among obligate biotrophic fungi in its capability to only partially degrade the host cell wall and in forming an astonishingly intricate interaction apparatus (IA) in its own hyphae, while the attacked host plant cell is triggered to produce a membranous bubble opposite the IA. However, no sequence data are currently available for this species. Based on molecular phylogenetic results obtained from complete SSU and partial LSU data, we show that the genus Cymadothea belongs to the Mycosphaerellaceae (Capnodiales, Dothideomycetes). This is the first report of sequences obtained for an obligate biotrophic member of Mycosphaerellaceae.
Tipo: Article / Letter to the editor Palavras-chave: Biotrophy; Capnodiales; Cymadothea trifolii; Dothideomycetes; GenomiPhi; LSU; Mycosphaerella kilianii; Mycosphaerellaceaesooty/black; Blotch of clover; SSU.
Ano: 2009 URL: http://www.repository.naturalis.nl/record/532112
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Dark septate endophytic pleosporalean genera from semiarid areas Naturalis
Knapp, D.G.; Kovács, G.M.; Zajta, E.; Groenwald, J.Z.; Crous, P.W..
Dark septate endophytes (DSE) are distributed worldwide as root-colonising fungi, and frequent in environments with strong abiotic stress. DSE is not a taxon, but constitutes numerous fungal taxa belonging to several orders of Ascomycota. In this study we investigate three unidentified DSE lineages belonging to Pleosporales that were found previously in semiarid sandy grasslands. For molecular phylogenetic studies seven loci (ITS, partial 18S nrRNA, 28S nrRNA, actin, calmodulin, transcription-elongation factor 1-α and ß-tubulin genes) were amplified and sequenced. Based on morphology and the resulting molecular phylogeny these isolates were found to represent three novel genera within the Pleosporales, namely Aquilomyces, Flavomyces and Darksidea, with eight...
Tipo: Article / Letter to the editor Palavras-chave: Dothideomycetes; Endophytes; Massarineae; Mating; Phylogeny; Sexual state; Taxonomy.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/588702
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Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi Naturalis
Gomes, R.R.; Glienke, C.; Videira, S.I.R.; Lombard, L.; Groenewald, J.Z.; Crous, P.W..
Diaporthe (Phomopsis) species have often been reported as plant pathogens, non-pathogenic endophytes or saprobes, commonly isolated from a wide range of hosts. The primary aim of the present study was to resolve the taxonomy and phylogeny of a large collection of Diaporthe species occurring on diverse hosts, either as pathogens, saprobes, or as harmless endophytes. In the present study we investigated 243 isolates using multilocus DNA sequence data. Analyses of the rDNA internal transcribed spacer (ITS1, 5.8S, ITS2) region, and partial translation elongation factor 1-alpha (TEF1), beta-tubulin (TUB), histone H3 (HIS) and calmodulin (CAL) genes resolved 95 clades. Fifteen new species are described, namely Diaporthe arengae, D. brasiliensis, D. endophytica,...
Tipo: Article / Letter to the editor Palavras-chave: Diaporthales; Diaporthe; Multi-Locus Sequence Typing (MLST); Phomopsis; Systematics.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/531720
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Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe Naturalis
Guarnaccia, V.; Groenewald, J.Z.; Woodhall, J.; Armengol, J.; Cinelli, T.; Eichmeier, A.; Ezra, D.; Fontaine, F.; Gramaje, D.; Gutierrez-Aguirregabiria, A.; Kaliterna, J.; Kiss, L.; Larignon, P.; Luque, J.; Mugnai, L.; Naor, V.; Raposo, R.; Sándor, E.; Váczy, K.Z.; Crous, P.W..
Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre- or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established...
Tipo: Article / Letter to the editor Palavras-chave: Canker; Multi-locus sequence typing; Pathogenicity; Vitis.
Ano: 2018 URL: http://www.repository.naturalis.nl/record/644417
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Dissoconiaceae associated with sooty blotch and flyspeck on fruits in China and the United States Naturalis
Li, H.Y.; Sun, G.Y.; Zhai, X.R.; Batzer, J.C.; Mayfield, D.A.; Crous, P.W.; Groenewald, J.Z.; Gleason, M.L..
Zasmidium angulare, a novel species of Mycosphaerellaceae, and several novel taxa that reside in Dissoconiaceae, were identified from a collection of apples and Cucurbita maxima (cv. Blue Hubbard) from China and the USA that exhibited sooty blotch and flyspeck (SBFS) signs on their host substrata. Morphology on fruit surfaces and in culture, and phylogenetic analyses of the nuclear ribosomal DNAs 28S and internal transcribed spacer regions, as well as partial translation elongation factor 1-alpha gene sequences in some cases, were used to delineate seven previously unidentified species and three known species. Pseudoveronaea was established as a new genus of Dissoconiaceae, represented by two species, P. ellipsoidea and P. obclavata. Although...
Tipo: Article / Letter to the editor Palavras-chave: Hyphomycetes; Malus; Microfungi; SBFS; Taxonomy.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/532388
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DNA barcoding of Mycosphaerella species of quarantine importance to Europe Naturalis
Quaedvlieg, W.; Groenewald, J.Z.; Jesús Yáñez-Morales, M. de; Crous, P.W..
The EU 7th Framework Program provided funds for Quarantine Barcoding of Life (QBOL) to develop a quick, reliable and accurate DNA barcode-based diagnostic tool for selected species on the European and Mediterranean Plant Protection Organization (EPPO) A1/A2 quarantine lists. Seven nuclear genomic loci were evaluated to determine those best suited for identifying species of Mycosphaerella and/or its associated anamorphs. These genes included β-tubulin (Btub), internal transcribed spacer regions of the nrDNA operon (ITS), 28S nrDNA (LSU), Actin (Act), Calmodulin (Cal), Translation elongation factor 1-alpha (EF-1α) and RNA polymerase II second largest subunit (RPB2). Loci were tested on their Kimura-2-parameter-based inter- and intraspecific variation, PCR...
Tipo: Article / Letter to the editor Palavras-chave: EPPO; Lecanosticta; Q-bank; QBOL.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/531831
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Elucidating the Ramularia eucalypti species complex Naturalis
Videira, S.I.R.; Groenewald, J.Z.; Kolecka, A.; Haren, L. van; Boekhout, T.; Crous, P.W..
The genus Ramularia includes numerous phytopathogenic species, several of which are economically important. Ramularia eucalypti is currently the only species of this genus known to infect Eucalyptus by causing severe leaf-spotting symptoms on this host. However, several isolates identified as R. eucalypti based on morphology and on nrDNA sequence data of the ITS region have recently been isolated from other plant hosts, from environmental samples and also from human clinical specimens. Identification of closely related species based on morphology is often difficult and the ITS region has previously been shown to be unreliable for species level identification in several genera. In this study we aimed to resolve this species-complex by applying a polyphasic...
Tipo: Article / Letter to the editor Palavras-chave: Mycosphaerellaceae; Plant pathogen; Species complex; Systematics.
Ano: 2015 URL: http://www.repository.naturalis.nl/record/560060
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Endophytic and pathogenic Phyllosticta species, with reference to those associated with Citrus Black Spot Naturalis
Glienke, C.; Pereira, O.L.; Stringari, D.; Fabris, J.; Kava-Cordeiro, V.; Galli-Terasawa, L.; Cunnington, J.; Shivas, R.G.; Groenewald, J.Z.; Crous, P.W..
We investigated the identity and genetic diversity of more than 100 isolates belonging to Phyllosticta (teleomorph Guignardia), with particular emphasis on Phyllosticta citricarpa and Guignardia mangiferae s.l. occurring on Citrus. Phyllosticta citricarpa is the causal agent of Citrus Black Spot and is subject to phytosanitary legislation in the EU. This species is frequently confused with a taxon generally referred to as G. mangiferae, the presumed teleomorph of P. capitalensis, which is a non-pathogenic endophyte, commonly isolated from citrus leaves and fruits and a wide range of other hosts. DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S nrDNA, ITS2) and partial translation elongation factor 1-alpha (TEF1), actin and...
Tipo: Article / Letter to the editor Palavras-chave: Guignardia endophyllicola; Guignardia mangiferae; Phyllosticta bifrenariae; Phyllosticta brazilianiae; Phyllosticta capitalensis; Phyllosticta citriasiana; Phyllosticta citribraziliensis; Phyllosticta citricarpa; Taxonomy.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/532033
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Epitypification of Fusarium oxysporum – clearing the taxonomic chaos Naturalis
Lombard, L.; Sandoval-Denis, M.; Lamprecht, S.C.; Crous, P.W..
Fusarium oxysporum is the most economically important and commonly encountered species of Fusarium. This soil-borne fungus is known to harbour both pathogenic (plant, animal and human) and non-pathogenic strains. However, in its current concept F. oxysporum is a species complex consisting of numerous cryptic species. Identification and naming these cryptic species is complicated by multiple subspecific classification systems and the lack of living ex-type material to serve as basic reference point for phylogenetic inference. Therefore, to advance and stabilise the taxonomic position of F. oxysporum as a species and allow naming of the multiple cryptic species recognised in this species complex, an epitype is designated for F. oxysporum. Using multi-locus...
Tipo: Article / Letter to the editor Palavras-chave: Cryptic species; Diversity; Human and plant pathogens; Species complex; Subspecific classification.
Ano: 2019 URL: http://www.repository.naturalis.nl/record/673170
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Exploring fungal mega-diversity: Pseudocercospora from Brazil Naturalis
Silva, M.; Barreto, R.W.; Pereira, O.L.; Freitas, N.M.; Groenewald, J.Z.; Crous, P.W..
Although the genus Pseudocercospora has a worldwide distribution, it is especially diverse in tropical and subtropical countries. Species of this genus are associated with a wide range of plant species, including several economically relevant hosts. Preliminary studies of cercosporoid fungi from Brazil allocated most taxa to Cercospora, but with the progressive refinement of the taxonomy of cercosporoid fungi, many species were relocated to or described in Pseudocercospora. Initially, species identification relied mostly on morphological features, and thus no cultures were preserved for later phylogenetic comparisons. In this study, a total of 27 Pseudocercospora spp. were collected, cultured, and subjected to a multigene analysis. Four genomic regions...
Tipo: Article / Letter to the editor Palavras-chave: Biodiversity; Capnodiales; Cercosporoid; Dothideomycetes; Multigene phylogeny; Mycosphaerellaceae; Plant pathogen; Systematics.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/611856
Registros recuperados: 77
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