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Enrichment methodology to increase the positivity of cultures from body fluids BJID
Daur,Alessandra Valle; Klimak Jr.,Francisco; Cogo,Laura Lúcia; Botão,Gislene Diógenes; Monteiro,Cristina Leise Bastos; Dalla Costa,Libera Maria.
Isolation and identification of etiological agents found in body fluids can be of critical importance for the recovery of patients suffering from potentially-severe infections, which are often followed by serious sequels. Eighty-two samples of different body fluids were analyzed using two different methods: (1) the conventional culture method (agar plating) and (2) the enrichment culture technique, using the Bact/Alert® blood culture bottle. The number of positive cultures increased on average from 9.7% to 23.1% with the enrichment culture technique. Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were the most frequently isolated bacteria. The enrichment method could provide a more accurate means the identifying etiological agents.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Body fluids; Etiological agents; Enrichment.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600002
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Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil BJID
Nogueira,Keite da Silva; Higuti,Ilma Hiroko; Nascimento,Agnaldo José do; Terasawa,Larissa Bail; Oliveira,Simone de; Matos,Adriana Pereira; Souza,Helena Aguilar Peres Homem de Mello de; Cogo,Laura Lúcia; Dalla Costa,Libera Maria.
Production of extended-spectrum beta-lactamases (ESBL) by enterobacteria is an important resistance mechanism against antimicrobial beta-lactamics. We tested 498 bacterial strains isolated from two tertiary-care teaching hospitals for ESBL production, using screening breakpoints for aztreonam and third generation cephalosporins, according to CLSI recommendations. Among these isolates, 155 were positive for the ESBL screening test, and 121 (78%) were confirmed by the clavulanic acid combination disk method. We found a high frequency of ESBL (24%) among Enterobacteriaceae, with a frequency of 57.4% for Klebsiella pneumoniae, 21.4% for Klebsiella oxytoca, and 7.2% for E. coli. In other members of Enterobacteriaceae, non-Klebsiella and non-E. coli, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESBL; Beta-lactamases; Enterobacteriaceae.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600006
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Quinolone-resistant Escherichia coli BJID
Ito,Carmen Antonia Sanches; Gales,Ana Cristina; Tognim,Maria Cristina B.; Munerato,Patrícia; Dalla Costa,Libera Maria.
Quinolones (nalidixic acid - NAL, norfloxacin - NOR, ciprofloxacin - CIP and gatifloxacin - GAT) were tested against Escherichia coli isolated from urine (385 patient samples) by disk diffusion (DD) and agar dilution (AD) methods. Fifty-three samples (13.8%) were classified as resistant to at least one of the quinolones tested. CIP and NOR susceptibilities were the same (91.4%) and they were similar to GAT (92.7%). Susceptibility to NAL, detected by the disk diffusion method, was used to predict susceptibility to NOR, CIP and GAT by the agar dilution method. The sensitivity and specificity of NAL were 100% and 95%, respectively. Twelve samples were analyzed for mutations in the quinolone resistance-determining region (QRDR) of the gyrA and parC genes....
Tipo: Info:eu-repo/semantics/other Palavras-chave: Urinary infection; Quinolone resistance; Escherichia coli.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100003
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