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Registros recuperados: 14
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Antagonistic effect of chitin-degrading microfungi on thick-shelled pig helminth eggs Organic Eprints
Katakam, KIran Kumar; Thapa, Sundar; Meyling, Nicholai V.; Dalsgaard, Anders; Roepstorff, Allan; Thamsborg, Stig Milan.
The rising demand for pigs produced in organic farming systems, which require access to outdoor areas and associated increased risk of gastrointestinal nematode infections, is creating growing interest in alternative control strategies. Also development of anthelminthic resistance due to extensive use of anthelmintics is a concern in intensive pig production. Use of chitin-degrading microfungi is one of the options. Extensive research has been conducted on biological control of plant pathogenic nematodes using such fungi, and some fungal species are commercially available for plant protection. In contrast, there are only limited laboratory studies conducted on eggs from nematodes which are animal parasites. The aim of present study is to investigate the...
Tipo: Conference paper, poster, etc. Palavras-chave: Health and welfare; Pigs.
Ano: 2011 URL: http://orgprints.org/20007/3/20007.pdf
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Contamination of lettuce with antibiotic resistant E. coli after slurry application Organic Eprints
Jensen, Annette Nygaard; Storm, Christina; Baggesen, Dorte Lau; Forslund, Anita; Dalsgaard, Anders.
Due to disease outbreaks associated with contaminated vegetables it has been speculated to what extent this may be linked with application of animal manure as fertilizer, which is particularly practiced in organic vegetable production where conventional fertilizers are prohibited. A field survey was therefore performed to assess the survival and transfer of antibiotic-resistant E. coli from animal manure to lettuces, with E. coli serving as an indicator of bacterial enteric pathogens.
Tipo: Conference paper, poster, etc. Palavras-chave: Food security; Food quality and human health Crop husbandry Farm nutrient management.
Ano: 2011 URL: http://orgprints.org/20418/3/20418.pdf
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Cryptosporidium and Giardia in organic pig farms: a longitudinal study of seasonal- and age-related variation in species, genotypes and excretion levels Organic Eprints
Petersen, Heidi Huus; Jianmin, Wang; Katakam, Kiran Kumar; Mejer, Helena; Ståhl, Marie; Dalsgaard, Anders; Olsen, Annette; Thamsborg, Stig Milan; Enemark, Heidi.
Although pigs are commonly infected with Cryptosporidium and Giardia duodenalis, and thus potential reservoirs of zoonotic species (or genotypes), little is known about age-related infection levels, seasonal differences and genetic variation in naturally infected pigs. Thus, to characterise seasonal and age-related variation in prevalence and intensity of infections at farm level, (oo)cyst shedding was monitored at quarterly intervals (September 2011 to June 2012) in piglets (n=152), young pigs (n=234), finishers (n=230) and sows (n=240) from three organic, Danish farms. (Oo)cysts were quantified by immunofluorescence microscopy; and representative Cryptosporidium (n=75) and Giardia (n=67) positive subsamples were characterized by amplification and partial...
Tipo: Journal paper Palavras-chave: Health and welfare.
Ano: 2014 URL: http://orgprints.org/27342/7/27342.pdf
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Development and survival of Ascaris suum eggs in deep litter of pigs Organic Eprints
Katakam, Kiran Kumar; Thamsborg, Stig Milan; Kyvsgaard, Niels Christian; Dalsgaard, Anders; Mejer, Helena.
Indoor transmission of Ascaris suum partly depends on the physico-chemical conditions in bedding material. Temperature,pH, aqueous ammonia, moisture, occurrence and development of A. suum eggs were therefore compared in different areas(resting, intermediate and latrine) of two deep litter pens on an organic farm in four seasons. There was some variation, but mean ammonia levels were generally very low (1·0–2·6mM) and pH levels were moderate (8·04–8·88) in all three areas. Relatively, resting areas were characterized by overall moderate moisture (36%) and moderately high temperature (35·7 °C)levels. The area contained few eggs (50 eggs g−1 DM) of which 17% were viable, and though only 4% were larvated and 0·7% appeared infective, it was more than in the...
Tipo: Journal paper Palavras-chave: Health and welfare.
Ano: 2014 URL: http://orgprints.org/27345/7/27345.pdf
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Escherichia coli Contamination of Lettuce Grown in Soils Amended with Animal Slurry Organic Eprints
Jensen, Annette Nygaard; Storm, Christina; Forslund, Anita; Baggesen, Dorte Lau; Dalsgaard, Anders.
A pilot study was conducted to assess the transfer of Escherichia coli from animal slurry fertilizer to lettuce, with E. coli serving as an indicator of fecal contamination and as an indicator for potential bacterial enteric pathogens. Animal slurry was applied as fertilizer to three Danish agricultural fields prior to the planting of lettuce seedlings. At harvest, leaves (25 g) of 10 lettuce heads were pooled into one sample unit (n ~ 147). Soil samples (100 g) were collected from one field before slurry application and four times during the growth period (n ~ 75). E. coli was enumerated in slurry, soil, and lettuce on 3M Petrifilm Select E. coli Count Plates containing 16 mg/liter streptomycin, 16 mg/liter ampicillin, or no antimicrobial agent. Selected...
Tipo: Journal paper Palavras-chave: Food security; Food quality and human health Composting and manuring Crop husbandry Crop health; Quality; Protection.
Ano: 2013 URL: http://orgprints.org/23106/7/23106.pdf
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Faecal contamination of lettuce heads after manure application Organic Eprints
Jensen, Annette Nygaard; Storm, Christina; Forslund, Anita; Baggesen, Dorte Lau; Dalsgaard, Anders.
In recent years, an increasing number of disease outbreaks have been associated with consumption of contaminated vegetables. Thus, it has been speculated to what extent such contamination is associated with application of animal manure as fertilizer, which is particularly practiced in organic vegetable production where conventional fertilizers are prohibited. A field survey was therefore performed aiming to assess the survival and transfer of E. coli from animal manure to lettuces, with E. coli serving as an indicator of bacterial enteric pathogens. Animal manure was applied to 3 Danish fields prior to planting of lettuce seedlings, then 5-8 weeks later at the normal time of harvest, inner and outer leafs of 10 lettuce heads were pooled into one sample...
Tipo: Conference paper, poster, etc. Palavras-chave: Food security; Food quality and human health.
Ano: 2010 URL: http://orgprints.org/20941/1/20941.pdf
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Forebyggelse af smitstoffer i økologisk frugt og grønt Organic Eprints
Lau Baggesen, Dorte; Nygaard Jensen, Annette; Dalsgaard, Anders.
Brugen af organisk husdyrgødning i økologisk produktion er nødvendig men indebærer også en risiko for forekomst og overførsel af smitstoffer som Salmonella, E. coli og virus. Et nyt fælleseuropæisk forskningsprojekt sætter fokus på smitstoffer i økologisk frugt og grønt. Målet er at finde produktionsrutiner, som kan mindske risiko for smitte og samtidig sikre en høj produktivitet.
Tipo: Newspaper or magazine article Palavras-chave: Food security; Food quality and human health.
Ano: 2007 URL: http://orgprints.org/15827/1/15827.pdf
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Leaching of Cryptosporidium parvum Oocysts, Escherichia coli, and a Salmonella enterica Serovar Typhimurium Bacteriophage through Intact Soil Cores following Surface Application and Injection of Slurry Organic Eprints
Forslund, Anita; Markussen, Bo; Toenner-Klank, Lise; Bech, Tina B.; Jacobsen, Ole Stig; Dalsgaard, Anders.
Increasing amounts of livestock manure are being applied to agricultural soil, but it is unknown to what extent this may be associated with contamination of aquatic recipients and groundwater if microorganisms are transported through the soil under natural weather conditions. The objective of this study was therefore to evaluate how injection and surface application of pig slurry on intact sandy clay loam soil cores influenced the leaching of Salmonella enterica serovar Typhimurium bacteriophage 28B, Escherichia coli, and Cryptosporidium parvum oocysts. All three microbial tracers were detected in the leachate on day 1, and the highest relative concentration was detected on the fourth day (0.1 pore volume). Although the concentration of the phage 28B...
Tipo: Journal paper Palavras-chave: Food security; Food quality and human health Air and water emissions Irrigation and drainage.
Ano: 2011 URL: http://orgprints.org/20435/4/20435.pdf
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Leaching of human pathogens in repacked soil lysimeters and contamination of potato tubers under subsurface drip irrigation in Denmark Organic Eprints
Forslund, Anita; Plauborg, Finn; Andersen, Mathias N.; Markussen, Bo; Dalsgaard, Anders.
The risk for contamination of potatoes and groundwater through subsurface drip irrigation with low quality water was explored in 30 large-scale lysimeters containing repacked coarse sand and sandy loam soils. The human pathogens, Salmonella Senftenberg, Campylobacter jejuni and E. coli O157:H7, and the virus indicator Salmonella Typhimurium bacteriophage 28B, were added weekly through irrigation tubes for one month with low irrigation rates (8 mm per week). In the following six months lysimeters were irrigated with groundwater free of pathogens. Two weeks after irrigation was started, phage 28B was detected in low concentrations (2 pfu ml-1) in leachate from both sandy loam soil and coarse sand lysimeters. After 27 days, phage 28B continued to be present...
Tipo: Journal paper Palavras-chave: Food security; Food quality and human health Irrigation and drainage.
Ano: 2011 URL: http://orgprints.org/18743/4/18743.pdf
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PREVALENCE OF THE PROTOZOAN PARASITE CRYPTOSPORIDIUM IN THREE ORGANIC PIG FARMS IN DENMARK Organic Eprints
Petersen, Heidi Huus; Mejer, Helena; Jianmin, Wang; Katakam, Kiran Kumar; Thamsborg, Stig Milan; Dalsgaard, Anders; Olsen, Annette; Enemark, Heidi L..
Pigs are potential sources of contamination with Cryptosporidium spp., which can lead to infection in humans. Cryptosporidiosis in humans is primarily caused by two species, C. hominis and the zoonotic C. parvum, however, other species including C. suis and C. scrofarum are able to cause zoonotic infection. The oocysts can survive for long periods in the environment and are able to resist most disinfectants. In order to estimate the prevalence of Cryptosporidium spp. in organic pigs and to improve knowledge of the epidemiology, the oocyst excretion was monitored at quarterly intervals during the period September 2011 to June 2012 in each of three organic, Danish pig farms. Faecal samples for examination of Cryptosporidium spp. were collected from 994 pigs...
Tipo: Conference paper, poster, etc. Palavras-chave: Health and welfare.
Ano: 2013 URL: http://orgprints.org/27379/7/27379.pdf
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Smitstoffer fra det ydre rum Organic Eprints
Dalsgaard, Anders; Jensen, Annette Nygaard; Baggesen, Dorte Lau.
Det ydre miljø er en vigtig kilde til smitstoffer i udendørs grise og økologiske grøntsager. Det viser resultater fra to forskningsprojekter på området.
Tipo: Newspaper or magazine article Palavras-chave: Food security; Food quality and human health Vegetables.
Ano: 2011 URL: http://orgprints.org/20312/13/20312.pdf
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Survival of Ascaris suum and Ascaridia galli eggs in liquidmanure at different ammonia concentrations andtemperatures Organic Eprints
Katakam, Kiran Kumar; Mejer, Helena; Dalsgaard, Anders; Kyyvsgaard, Niels Christian; Thamsborg, Stig Milan.
tEggs of Ascaris suum from pigs are highly resistant and commonly used as a conservativeindicator of pathogen inactivation during slurry storage. Eggs of Ascaridia galli, the poultryascarid, are also known to be highly resistant but the suitability as an indicator of pathogeninactivation has never been tested. Pig slurry has to be stored for several months to inac-tivate pathogens but chemical treatment of slurry may reduce this time. The suitability ofA. galli as an indicator of slurry sanitation was tested by comparing the survival of eggsof A. suum and A. galli in pig slurry. In addition, the effect of urea treatment on inactiva-tion of ascarid eggs in relation to storage time was also tested. Nylon bags with 10,000eggs of either species were placed in...
Tipo: Journal paper Palavras-chave: Health and welfare.
Ano: 2014 URL: http://orgprints.org/27344/7/27344.pdf
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Transmission and control of the parasite Ascaris suum (large round worm) in Danish organic farms Organic Eprints
Mejer, Helena; Katakam, Kiran Kumar; Gautam, Susmita; Dalsgaard, Anders; Thamsborg, Stig Milan.
Ascaris suum is the most common intestinal worm in pigs irrespective of production system. However, organic farms which promote animal welfare (e.g. by providing pastures and bedding material) may further promote survival of the eggs through which the parasite is dispersed to new hosts. The parasite has a negative effect on production results and possibly impairs vaccines. A one year survey of 5 Danish organic farms therefore aimed at mapping the occurrence of parasite eggs in the farm environment, supplemented with laboratory and field studies, including serial necropsies of different age groups of pigs from 2 farms to monitor infection levels. The combined results showed that eggs were present on farrowing and weaning pastures, ensuring continuous...
Tipo: Conference paper, poster, etc. Palavras-chave: Health and welfare.
Ano: 2013 URL: http://orgprints.org/27346/7/27346.pdf
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Transmission dynamics of Ascaris suum in Danish organic pig farms Organic Eprints
Katakam, Kiran Kumar; Thamsborg, Stig Milan; Dalsgaard, Anders; Mejer, Helena.
Although Ascaris suum is the most common nematode in pig farms, the on-farm transmission dynamics is not well described. Hence we performed a 1-year field study on organic pig farms, mapping egg contamination levels in pens and on pastures (dry sows, lactating sows and starters) and infection levels in animals. In conclusion, recovery of infective eggs was limited in deep litter compared to shallow litter, but many deep litter eggs were still viable. A. suum transmission takes place both indoors and outdoors. Highly infective bedding material should be stored, composted or treated before spreading on crops and never used for pastures.
Tipo: Journal paper Palavras-chave: Health and welfare.
Ano: 2014 URL: http://orgprints.org/27394/7/27394.pdf
Registros recuperados: 14
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