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Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags ArchiMer
Cousin, Xavier; Daouk, Tarek; Pean, Samuel; Lyphout, Laura; Schwartz, Marie-elise; Begout, Marie-laure.
Although individual electronic tagging using passive integrated acoustic (PIT) tags is established, it is mainly for fish >60. mm in length and is unsuitable for fish of <30. mm, like zebrafish. We used radio frequency identification (RFID) microtags (1 mm in diameter and 6 mm in length, with a mass of similar to 10 mg) to individually identify juvenile zebrafish (length 16-42 mm, mass 138-776 mg) for the first time, and studied the effects of intracoelomic implantation on fish survival and microtag loss, growth, spawning and exploratory behaviour. After 5.5 months, both high survival (82%) and low microtag loss (11%) were achieved. The smallest surviving fish weighed 178 mg, and success in microtag reading was 73% for the size class 350-450 mg (26...
Tipo: Text Palavras-chave: Effects; Fish; Marking.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00089/20066/17835.pdf
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Long-term dietary-exposure to non-coplanar PCBs induces behavioral disruptions in adult zebrafish and their offspring ArchiMer
Pean, Samuel; Daouk, Tarek; Vignet, Caroline; Lyphout, Laura; Leguay, Didier; Loizeau, Veronique; Begout, Marie-laure; Cousin, Xavier.
The use of polychlorinated biphenyls (PCBs) has been banned for several decades. PCBs have a long biological half-life and high liposolubility which leads to their bioaccumulation and biomagnification through food chains over a wide range of trophic levels. Exposure can lead to changes in animal physiology and behavior and has been demonstrated in both experimental and field analyses. There are also potential risks to high trophic level predators, including humans. A maternal transfer has been demonstrated in fish as PCBs bind to lipids in eggs. In this study, behavioral traits (exploration and free swimming, with or without challenges) of contaminated zebrafish (Danio rerio) adults and their offspring (both as five-day-old larvae and as two-month-old fish...
Tipo: Text Palavras-chave: Exploration; Swimming activity; Organic pollutant effects; Generation; Video analysis.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27799/26068.pdf
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Long-term food-exposure of zebrafish to PCB mixtures mimicking some environmental situations induces ovary pathology and impairs reproduction ability ArchiMer
Daouk, Tarek; Larcher, Thibaut; Roupsard, Francois; Lyphout, Laura; Rigaud, Cyril; Ledevin, Mireille; Loizeau, Veronique; Cousin, Xavier.
Although the use of polychlorinated biphenyls (PCBs) has been banned for several decades, they are still present in the environment and are occasionally mechanically released from sediment or transferred through the trophic chain. Field analyses have established correlations between exposure to PCBs and alterations in fish physiology including reproductive function. Experimental exposures have been mainly performed using dioxin-like PCBs or other congeners at very high concentrations. However, these studies are often difficult to relate to real-life conditions. In the present study, we performed a life-cycle exposure using zebrafish model and mixtures representative of some environmental situations in terms of doses, composition and containing mainly non...
Tipo: Text Palavras-chave: Zebrafish; PCBs; Chronic exposure; Physiology; Reproduction; Atresia.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00059/17038/14608.pdf
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