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Biological activity of Serratia marcescens cytotoxin 56
Carbonell,G.V.; Amorim,C.R.N.; Furumura,M.T.; Darini,A.L.C.; Fonseca,B.A.L.; Yano,T..
Serratia marcescens cytotoxin was purified to homogeneity by ion-exchange chromatography on a DEAE Sepharose Fast Flow column, followed by gel filtration chromatography on a Sephadex G100 column. The molecular mass of the cytotoxin was estimated to be about 50 kDa. Some biological properties of the cytotoxin were analyzed and compared with well-characterized toxins, such as VT1, VT2 and CNF from Escherichia coli and hemolysin produced by S. marcescens. The sensitivity of the cell lines CHO, HeLa, HEp-2, Vero, BHK-21, MA 104 and J774 to the cytotoxin was determined by the cell viability assay using neutral red. CHO and HEp-2 were highly sensitive, with massive cellular death after 1 h of treatment, followed by BHK-21, HeLa, Vero and J774 cells, while MA 104...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell culture; Biological activity; Serratia marcescens; Cytotoxin; Virulence factors.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000300010
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Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens 56
Carbonell,G.V.; Alfieri,A.F.; Alfieri,A.A.; Vidotto,M.C.; Levy,C.E.; Darini,A.L.C.; Yanaguita,R.M..
Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells. These strains were isolated from distinct clinical sources and classified into seven different serotypes (O1:H7; O4:NM; O10:NT; O19:NM; O6,14:H4; O6,14:NM and O6,14:H1). No relationship was observed between cytotoxic activity and clinical source or serotypes of the strains. Plasmids from five cytotoxin-producing S. marcescens strains were transferred to E. coli K12/711. The transconjugants did not exhibit cytotoxicity, indicating that the cytotoxic effect is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotoxin production; Nosocomial infections; Plasmids; Serratia marcescens; Virulence factors.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001100005
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Enterococcus gallinarum carrying the vanA gene cluster: first report in Brazil 56
Camargo,I.L.B.C.; Barth,A.L.; Pilger,K.; Seligman,B.G.S.; Machado,A.R.L.; Darini,A.L.C..
In 2000, Enterococcus faecalis resistant to vancomycin was first reported at a tertiary hospital in Porto Alegre, southern Brazil. The resistance spread to other hospitals and surveillance programs were established by hospital infection committees to prevent the spread of vancomycin-resistant enterococci. In February 2002, an isolate initially identified at the genus level as Enterococcus was obtained by surveillance culture (rectal swab) from a patient admitted to a hospital for treatment of septic arthritis in the shoulder. The isolate proved to be resistant to vancomycin by the disc diffusion method and confirmed by an E-test resulting in a minimal inhibitory concentration of > or = 256 µg/ml. This isolate was sent to a reference laboratory...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Enterococcus gallinarum; VanA phenotype; Vancomycin; Resistance.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001100012
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Phenotyping and genotyping methods applied to investigate the relatedness of Brazilian isolates of Enterobacter cloacae 56
Darini,A.L.C.; Magalhães,V.D.; Levy,C.L.; Barth,A.L.; Coscina,A.L..
In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-serotyping. Plasmid profile analysis, ribotyping, and macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) were used as genotyping methods. Sero- and phage-typing were not useful since the majority of isolates could not be subtyped by these methods. Biotyping, antibiogram and plasmid profile permitted us to classify the samples into different groups...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Enterobacter cloacae; Phage-typing; Serotyping; Ribotyping; Pulsed-field gel electrophoresis; Nosocomial infection.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900004
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