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Registros recuperados: 9
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A Targeted Metagenomics Approach to Study the Diversity of Norovirus GII in Shellfish Implicated in Outbreaks 5
Desdouits, Marion; Wacrenier, Candice; Ollivier, Joanna; Schaeffer, Julien; Le Guyader, Soizick.
Human noroviruses (NoV) cause epidemics of acute gastroenteritis (AGE) worldwide and can be transmitted through consumption of contaminated foods. Fresh products such as shellfish can be contaminated by human sewage during production, which results in the presence of multiple virus strains, at very low concentrations. Here, we tested a targeted metagenomics approach by deep-sequencing PCR amplicons of the capsid (VP1) and polymerase (RdRp) viral genes, on a set of artificial samples and on shellfish samples associated to AGE outbreaks, to evaluate its advantages and limitations in the identification of strains from the NoV genogroup (G) II. Using artificial samples, the method allowed the sequencing of most strains, but not all, and displayed variability...
Tipo: Text Palavras-chave: Norovirus; Foodborne virus; Metagenomics; Amplicon deep sequencing; Viral diversity.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00646/75831/76818.pdf
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Contamination of Clams with Human Norovirus and a Novel Hepatitis A Virus in Cameroon 5
Bonny, Patrice; Desdouits, Marion; Schaeffer, Julien; Garry, Pascal; Essia Ngang, Jean Justin; Le Guyader, Soizick.
Shellfish constitute an important protein source but may be contaminated by viruses from various origins. A study performed on clams collected in Cameroon showed a high prevalence of norovirus and hepatitis A virus. After sequencing, the hepatitis A virus showed similarities with the genotype V simian strains. The need for a one-health approach to protect humans from emerging diseases is evident by the high number of microbial pathogens that become zoonotic following insect bites or consumption of contaminated meat. This is particularly well documented for infectious disease transmission from non-human primates to human (Devaux et al. 2019). Environmental issues are critical in such events, especially for RNA viruses that may be excreted at high...
Tipo: Text Palavras-chave: Shellfish; Hepatitis A virus; Molecular epidemiology.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00635/74745/74851.pdf
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De la terre à la mer, les microbes sous surveillance 5
Desdouits, Marion; Piquet, Jean-come; Rocq, Sophie; Euller, Gabriel; Le Guyader, Soizick; Garry, Pascal.
Ce poster a été réalisé dans le cadre de la fête de la science 2020, au muséum d’histoire naturelle de Nantes, afin de présenter au grand public l’évolution de la surveillance de la qualité microbiologique des zones de production conchylicole depuis le début du 20ème siècle à nos jour.
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00675/78756/80928.pdf
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De la terre à la mer. "Usual suspects" de la contamination microbiologique des coquillages 5
Euller, Gabriel; Desdouits, Marion; Pilven, Sophie; Le Guyader, Soizick; Garry, Pascal.
Ce poster a été réalisé dans le cadre de la fête de la science 2020, au muséum d’histoire naturelle de Nantes, afin de présenter au grand public les principaux organismes pathogènes pour l’homme contaminant les coquillages.
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00675/78754/80922.pdf
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Metagenomic to evaluate norovirus genomic diversity in oysters: Impact on hexamer selection and targeted capture-based enrichment 5
Strubbia, Sofia; Schaeffer, Julien; Besnard, Alban; Wacrenier, Candice; Le Mennec, Cecile; Garry, Pascal; Desdouits, Marion; Le Guyader, Soizick.
Human virus transmission through food consumption has been identified since many years and the international trade increases the risk of dissemination of viral pathogens. The development of metagenomic approach holds many promises for the surveillance of viruses in food and water. This work aimed to analyze norovirus diversity and to evaluate strain-dependent accumulation patterns in three oyster types by using a metagenomic approach. Different hexamer sets to prime cDNA were evaluated before capture-based approach to enhance virus reads recovery during deep sequencing. The study includes the use of technical replicates of artificially contaminated oysters and the analysis of multiple negatives controls. Results showed a clear impact of the hexamer set...
Tipo: Text Palavras-chave: Norovirus; Oysters; Metagenomic; Human enteric viruses..
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00614/72575/71535.pdf
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Novel opportunities for NGS-based one health surveillance of foodborne viruses 5
Desdouits, Marion; De Graaf, Miranda; Strubbia, Sofia; Oude Munnink, Bas B.; Kroneman, Annelies; Le Guyader, Soizick; Koopmans, Marion P. G..
Foodborne viral infections rank among the top 5 causes of disease, with noroviruses and hepatitis A causing the greatest burden globally. Contamination of foods by infected food handlers or through environmental pollution are the main sources of foodborne illness, with a lesser role for consumption of products from infected animals. Viral partial genomic sequencing has been used for more than two decades to track foodborne outbreaks and whole genome or metagenomics next-generation-sequencing (NGS) are new additions to the toolbox of food microbiology laboratories. We discuss developments in the field of targeted and metagenomic NGS, with an emphasis on application in food virology, the challenges and possible solutions towards future routine application
Tipo: Text Palavras-chave: Next-generation sequencing; Metagenomics; Foodborne virus; Food virology; Human enteric virus; Norovirus.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00641/75330/75988.pdf
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Rapport d’activités 2018 – Laboratoire Santé Environnement et Microbiologie. 5
Desdouits, Marion; Garry, Pascal; Gourmelon, Michele; Hervio Heath, Dominique; Hubert, Francoise; Kaelin, Gaelle; Kergaravat, Cedric; Le Mennec, Cecile; Le Guyader, Soizick; Loiseau, Veronique; Lozach, Solen; Maillot, Jessica; Ollivier, Joanna; Parnaudeau, Sylvain; Piquet, Jean-come; Quenot, Emmanuelle; Rocq, Sophie; Schaeffer, Julien; Serghine, Joelle; Vallade, Emilie; Veron, Antoine; Wacrenier, Candice.
Ce rapport présente une synthèse des actions, et travaux réalisés par le laboratoire Santé Environnement et Microbiologie pendant l’année 2018. Les divers projets de recherche sont résumés et les derniers développements ou résultats sont brièvement évoqués. Les actions en tant que Laboratoire National de Référence pour la Microbiologie des Coquillages et les activités pour la coordination du réseau REMI sont également présentées.
Tipo: Text Palavras-chave: Microbiologie sanitaire; Bactéries entériques; Vibrions; Virus entériques humains; Norovirus; Coquillage; Activité de référence; REMI..
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00491/60218/63584.pdf
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Role of Gag and lipids during HIV-1 assembly in CD4(+) T cells and macrophages 5
Mariani, Charlotte; Desdouits, Marion; Favard, Cyril; Benaroch, Philippe; Muriaux, Delphine M..
HIV-1 is an RNA enveloped virus that preferentially infects CD4(+) T lymphocytes and also macrophages. In CD4(+) T cells, HIV-1 mainly buds from the host cell plasma membrane. The viral Gag polyprotein targets the plasma membrane and is the orchestrator of the HIV assembly as its expression is sufficient to promote the formation of virus-like particles carrying a lipidic envelope derived from the host cell membrane. Certain lipids are enriched in the viral membrane and are thought to play a key role in the assembly process and the envelop composition. A large body of work performed on infected CD4(+) T cells has provided important knowledge about the assembly process and the membrane virus lipid composition. While HIV assembly and budding in macrophages is...
Tipo: Text Palavras-chave: HIV-1; Lipids; Assembly; Gag; CD4(+) T cells; Macrophages.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00344/45490/45040.pdf
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VirOdyssée, la course du norovirus de la terre à la mer 5
Euller, Gabriel; Desdouits, Marion; Pilven, Sophie; Preux, Guillemette; Le Guyader, Soizick; Garry, Pascal.
Ce jeu de l’oie sous forme de poster a été réalisé dans le cadre de la fête de la science 2020, au muséum d’histoire naturelle de Nantes, afin de présenter au grand public, le mode de dissémination d’un norovirus depuis son excrétion jusqu’à la contamination des coquillages et l’impact de l’homme et de l’environnement sur le devenir de ce virus.
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00675/78757/80929.pdf
Registros recuperados: 9
Primeira ... 1 ... Última
 

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