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Registros recuperados: 10
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Avaliação da eficiência de três ágares seletivos no isolamento de Listeria monocytogenes Ciênc. Tecnol. Aliment.
Rodrigues,Denize Aparecida; Franco,Bernadette Dora Gombossi de Melo; Landgraf,Mariza; Destro,Maria Teresa.
A variedade de protocolos existentes para a pesquisa de Listeria sp em alimentos e outras amostras é muito grande, o que dificulta a escolha daquele que possa apresentar melhores resultados. Os protocolos recomendados nas metodologias tradicionais indicam vários caldos de pré-enriquecimento e enriquecimento seletivo, assim como meios de isolamento seletivo. O presente estudo teve por finalidade comparar a eficiência dos ágares cloreto de lítio-feniletanol-moxalactam (LPM), PALCAM (PAL) e do ágar hemolítico ceftazidima-cloreto de lítio (HCLA) no isolamento de L. monocytogenes a partir de diferentes tipos de amostras colhidas em uma linha de processamento de "nuggets" congelados de frango. Um total de 413 amostras foi examinado. As amostras foram...
Tipo: Info:eu-repo/semantics/article Palavras-chave: L. monocytogenes; Isolamento; HCLA; PALCAM; LPM.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612003000400016
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Avaliação da qualidade microbiológica de ovo integral pasteurizado produzido com e sem a etapa de lavagem no processamento Ciênc. Tecnol. Aliment.
Aragon-Alegro,Lina Casale; Souza,Kátia Leani de Oliveira; Costa Sobrinho,Paulo de Souza; Landgraf,Mariza; Destro,Maria Teresa.
Microrganismos patogênicos podem contaminar ovos em diferentes estágios do processamento. Na tentativa de reduzir problemas decorrentes dessa contaminação por microrganismos patogênicos e/ou deteriorantes, os ovos são submetidos a processos como lavagem da casca e pasteurização. Vários estudos mostraram que alguns agentes químicos utilizados nessa lavagem podem causar danos físicos ao produto, facilitando a entrada de microrganismos através da casca. O nosso objetivo foi avaliar a eficiência da lavagem dos ovos, anteriormente à quebra, na redução da contaminação de ovo integral líquido. Foram colhidas amostras de ovo integral em 3 pontos da linha de produção, provenientes de ovos lavados e não lavados. Foram feitas pesquisa de Salmonella sp e enumeração de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovos; Lavagem; Qualidade microbiológica.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000300036
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Behaviour of L. monocytogenes in sliced, vacuum-packed mortadella BJM
Bersot,Luciano dos Santos; Gillio,Cíntia; Tavolaro,Paula; Landgraf,Mariza; Franco,Bernadette Dora Gombossy de Melo; Destro,Maria Teresa.
This study evaluated the growth of naturally occurring L. monocytogenes in sliced, vacuum-packed mortadella samples during storage at 5ºC until the expiration date. Tukey's test indicated that counts of L. monocytogenes on 0, 10, 20, 30 and 40 days of storage were significantly different (p<0.05), indicating growth during shelf life. In three trials, the mean increase was 1.72 log cycles. Vacuum packing and storage under refrigeration were not effective in controlling the growth of L. monocytogenes in sliced mortadella, indicating that good manufacturing practices and implemented HACCP programs are essential to assure safety of this product.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Growth; Sliced mortadella; Vacuum-packing.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300020
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Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms BJM
Silva,Wladimir Padilha da; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette D.G.M..
This study reports results on the biochemical characteristics of 274 Staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. Features included colony morphology on Baird Parker agar (BPA), catalase, coagulase and thermonuclease tests. API-Staph (bioMérieux, France) was used as reference identification system. 39.4% of the colonies on BPA identified as S.aureus were atypical. They were more frequent in milk samples (94.4%). All S.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. The majority of atypical S.aureus strains presented a 4+ coagulase result. 2.2% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Atypical S.aureus; Coagulase; Thermonuclease; API-Staph; Baird-Parker agar; Milk; Mastitis; Environmental samples.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200008
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Characterization of Listeria monocytogenes isolated from a fresh mixed sausage processing line in Pelotas-RS by PFGE BJM
von Laer,Ana Eucares; Lima,Andréia Saldanha de; Trindade,Paula dos Santos; Andriguetto,Cristiano; Destro,Maria Teresa; Silva,Wladimir Padilha da.
Listeria monocytogenes is a bacterium capable to adhere to the surfaces of equipment and utensils and subsequently form biofilms. It can to persist in the food processing environmental for extended periods of time being able to contaminate the final product. The aim of this study was to trace the contamination route of L. monocytogenes on a fresh mixed sausage processing line, from raw material to the final product. The isolates obtained were characterized by serotyping and molecular typing by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes ApaI and AscI. L. monocytogenes was detected in 25% of the samples. The samples of raw material were not contaminated, however, the microorganism was detected in 21% of the environmental samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; PFGE; Serotyping; Persistent strains.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300021
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Inactivation of Escherichia coli O157:H7 in hamburgers by gamma irradiation BJM
Chirinos,Rodolfo R.O.; Vizeu,Dirceu M.; Destro,Maria Teresa; Franco,Bernadette D.G.M.; Landgraf,Mariza.
Escherichia coli O157:H7 causes bloody diarrhoea, haemorrhagic colitis and life-threatening complications like haemolytic uremic syndrome and thrombotic thrombocitopenic purpura. Among foods associated with outbreaks caused by this pathogen, hamburger is the most common one. The aim of this research was to determine the radiation dose to reduce the population of E. coli O157:H7 in hamburgers to non-detectable levels in order to render a safer product. Hamburgers, inoculated with Escherichia coli O157:H7, were exposed to gamma radiation (60Co) treatment, with doses ranging from 0 to 0.7 kGy. The average temperature during the process was 5.6ºC. Non-inoculated hamburgers were submitted to sensory evaluation after being exposed to irradiation doses of 0.8 kGy...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hamburger; Patties; Escherichia coli O157:H7; Food irradiation; Gamma radiation.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100011
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Performance of two ready-to-use systems for enumeration of aerobic mesophilic microorganisms in frozen goat milk BJM
Tavolaro,Paula; Ferrati,Analí Ramazotti; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette Dora Gombossy de Melo.
Enumeration of aerobic microorganisms in Brazilian pasteurized bovine milk using PetrifilmTM AC and SimplateTM TPC was shown to be influenced by the nature of autochthonous microbiota. This study was conducted to assess if the same occurs in pasteurized goat milk. Pasteurized frozen goat milk (n=62) was purchased in retail stores in São Paulo, SP, Brazil, and submitted to mesophilic aerobic counts using PetrifilmTM AC and SimplateTM TPC, comparing results with those of conventional pour-plates. Correlation indexes above 0.90 were ranked "excellent"; those between 0.80 and 0.89, "good", those from 0.79 to 0.50, "fair", and those below 0.50, "poor". Correlation indexes for PetrifilmTM AC counts were "good", but "poor" using SimplateTM TPC. Comparison of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rapid methods; Ready-to-use systems; Goat milk; PetrifilmTM AC; SimplateTM TPC.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300017
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Pheno- and genotypic characterization of Listeria monocytogenes clinical isolates from the southwestern region of the State of São Paulo, Brazil BJM
Lemes-Marques,Eneida Gonçalves; Cruz,Cristina Durante; Destro,Maria Teresa.
Considering the lack of information available in Brazil concerning listeriosis, the present study aimed to analyze phenotypic and genotypically Listeria monocytogenes clinical isolates from the Southwestern region of the State of São Paulo, Brazil. From January 1995 to May 2005, thirteen isolates of L. monocytogenes from twelve patients with listeriosis were sent to the Adolfo Lutz Institute (Campinas Regional Laboratory, São Paulo, Brazil). The antimicrobial susceptibility of the isolates was evaluated by the microdilution broth method for ampicillin, gentamicin, trimethoprim, sulfamethoxazole and vancomycin. They were also serotyped (Denka Seiken, Japan), and sub-typed by PFGE employing ApaI and AscI and the American CDC protocol. None of the isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Serotypes; Antibiotic resistance; Genotypic characterization; Listeriosis.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200019
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SENSORY ASPECTS AND REDUCTION OF Salmonella IN IRRADIATED EGG POWDER Ciência e Agrotecnologia
Froehlich,Ângela; Franco,Bernadette Dora Gombossy de Melo; Destro,Maria Teresa; Landgraf,Mariza.
ABSTRACTEggs and their products have been implicated in food-borne disease outbreaks due to contamination with SalmonellaEnteritidis. Irradiation, as a food preservation technology, could minimize such contamination. The aim of this study was to determine the effects of irradiation in powdered egg yolk, egg white, and whole egg samples spiked with Salmonella Enteritidis. The powdered egg samples were exposed to doses of 0.5, 1.0, 1.0, 2.0, 2.5, 3.0, 3.5, or 4.0 kGy irradiation. Odors and tastes of raw and cooked non-inoculated, irradiated samples, and non-irradiated samples, were analyzed by a trained panel. A dose of 2.0 kGy resulted in slight to moderate changes in powdered egg yolk samples, compared to non-irradiated controls, while minimal differences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteria; Elimination; Powder food.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500506
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Shelf life of irradiated minimally processed (MP) watercress (Nasturtium officinale) Ciênc. Tecnol. Aliment.
Martins,Cecília Geraldes; Behrens,Jorge Herman; Aragon-Alegro,Lina Casale; Vieira,Vanessa Santos; Costa-Sobrinho,Paulo Souza; Vizeu,Dirceu Martins; Hutzler,Beatriz; Franco,Bernadette Dora Gombossy de Melo; Destro,Maria Teresa; Landgraf,Mariza.
This study was carried out to evaluate the shelf life of minimally processed (MP) watercress exposed to gamma radiation with doses of 1.0; 3.0; 4.0 kGy. Packaged irradiated and non-irradiated MP watercress was subject to sensory analysis. A panel consisting of 25-30 non-trained members, aged 20-55 years, was used. Sensory evaluation was carried out on days 0, 2, 5, 7, 9 and 12 after treatment with packages maintained at 7 °C. The members of the panel rated each sample for overall liking on a hybrid 10 hedonic scale (0 = extremely dislike; 10 = extremely like). Microbiological analyses were also conducted. Compared to the non-irradiated sample, 1 kGy irradiated watercress increased its shelf life by one day (16 days). Shelf lives of samples exposed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Watercress; Vegetable irradiation; Acceptance test.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612007000100008
Registros recuperados: 10
Primeira ... 1 ... Última
 

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