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Determination of myocardial infarction size in rats by echocardiography and tetrazolium staining: correlation, agreements, and simplifications BJMBR
Dos Santos,L.; Mello,A.F.S.; Antonio,E.L.; Tucci,P.J.F..
Triphenyltetrazolium chloride (TTC) staining and echocardiography (ECHO) are methods used to determine experimental myocardial infarction (MI) size, whose practical applicability should be expanded. Our objectives were to analyze the accuracy of ECHO in determining infarction size in rats during the first days following coronary occlusion and to test whether a simplified single measurement by TTC correctly indicates MI size, as determined by the average value for multiple slices. Infarction was induced in female Wistar rats by coronary artery occlusion and MI size analysis was performed after the acute (7th day) and chronic periods (after 4 weeks) by ECHO matched with TTC. ECHO and TTC showed similar values of MI size (% of left ventricle perimeter) in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Myocardial infarction size; Echocardiography and triphenyltetrazolium comparison; Bland-Altman test.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000300004
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Drag reduction by polyethylene glycol in the tail arterial bed of normotensive and hypertensive rats BJMBR
Bessa,K.L.; Belletati,J.F.; Dos Santos,L.; Rossoni,L.V.; Ortiz,J.P..
This study was designed to evaluate the effect of drag reducer polymers (DRP) on arteries from normotensive (Wistar) and spontaneously hypertensive rats (SHR). Polyethylene glycol (PEG 4000 at 5000 ppm) was perfused in the tail arterial bed with (E+) and without endothelium (E-) from male, adult Wistar (N = 14) and SHR (N = 13) animals under basal conditions (constant flow at 2.5 mL/min). In these preparations, flow-pressure curves (1.5 to 10 mL/min) were constructed before and 1 h after PEG 4000 perfusion. Afterwards, the tail arterial bed was fixed and the internal diameters of the arteries were then measured by microscopy and drag reduction was assessed based on the values of wall shear stress (WSS) by computational simulation. In Wistar and SHR groups,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shear stress; Flow-pressure response; Drag reduction; Endothelium; Polyethylene glycol; Hypertension.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800006
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