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Gene transcription in Lactarius quietus-Quercus petraea ectomycorrhizas from a forest soil Inra
Courty, P.E.; Poletto, M; Duchaussoy, F.; Buee, M.; Garbaye, J.; Martin, F..
Extracting fungal mRNA from ectomycorrhizas (ECMs) and forest soil samples for monitoring in situ metabolic activities is a significant challenge when studying the role of ECMs in biogeochemical cycles. A robust, simple, rapid, and effective method was developed for extracting RNA from rhizospheric soil and ECMs by adapting previous grinding and lysis methods. The quality and yield of the extracted RNA were sufficient to be used for reverse transcription. RNA extracted from ECMs of Lactarius quietus in a 100-year-old oak stand was used to construct a cDNA library and sequence expressed sequence tags. The transcripts of many genes involved in primary metabolism and in the degradation of organic matter were found. The transcription levels of four...
Tipo: Journal Article Palavras-chave: ECTOMYCORHIZE AMINO ACIDS; ECTOMYCORRHIZAS; FOREST SOILS; GENE EXPRESSION; GLUTAMATE-AMMONIA LIGASE; METABOLISM; MYCORRHIZAL FUNGI; N-ACETYL-BETA-GLUCOSAMINIDASE; RHIZOSPHERE; RNA; SOIL TYPES; BETA-N-ACETYLGLUCOSAMINIDASE; DNA TRANSCRIPTION; GLUTAMINE SYNTHETASE; RIBONUCLEIC ACID; TYROSINASE.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e4344c33&uri=/notices/prodinra1/2010/11/
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Genome-wide identification of NBS resistance genes in Populus trichocarpa Inra
Kohler, A.; Rinaldi, C.; Duplessis, S.; Baucher, M.; Geelen, D.; Duchaussoy, F.; Meyers, BC; Boerjan, W.; Martin, F..
As the largest class of disease resistance R genes, the genes encoding nucleotide binding site and leucine-rich repeat proteins (“NBS-LRR genes”) play a critical role in defending plants from a multitude of pathogens and pests. The diversity of NBS-LRR genes was examined in the Populus trichocarpa draft genome sequence. The NBS class of genes in this perennial tree is large and diverse, comprised of ∼400 genes, at least twice the complement of Arabidopsis. The NBS family can be divided into multiple subfamilies with distinct domain organizations. It includes 119 Coiled-Coil-NBS-LRR genes, 64 TIR-NBS-LRR genes, 34 BED-finger-NBS-LRR, and both truncated and unusual NBS- and NBS-LRR-containing genes. The transcripts of only 34 NBS-LRR genes were detected in...
Tipo: Journal Article Palavras-chave: Populus; NBS-LRR; Resistance.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010b08a4238&uri=/notices/prodinra1/2010/10/
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