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| Registros recuperados: 10 | |
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| Stewien,Klaus Eberhard; Lima,Lourdes Rehder de Andrade Vaz de; Botosso,Viviane Fongaro; Oliveira,Maria Isabel de; Fagundes,Simone N.; Nogueira,Meri B.; Ragazzi,Selma Lopes Betta; Costa,Maria Tereza Zuluni da; Ejzenberg,Bernardo; Durigon,Edison Luiz. |
| A clinical and laboratory evaluation of 11 children and young adults with measleslike rash was done during the measles outbreak in the Greater São Paulo Metropolitan area at the end of 1996 and spread over the country during 1997. Measles was laboratory confirmed in 07 patients by specific IgM detection in acute serum specimens using an IgM-capture EIA, by specific IgG seroconversion in serum pairs, and by reverse transcription PCR and virus isolation in peripheral blood lymphocytes. Clinical presentations were not always classic; one of the 07 cases had received measles vaccine and corresponded to modified clinical case of measles. The 4 remaining cases were negative for measles and were diagnosed as exanthem subitum (2 cases), scarlet fever and Kawasaki... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Measles; Clinical evaluation; Laboratory investigation; Polymerase chain reaction; Measles virus isolation; Enzyme immunoassay. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400008 |
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| Campos,Angélica Cristine de Almeida; Durigon,Edison Luiz; Leal,Andréa Lima; Silva,Tereza Souza; Bosso,Patrícia Alves Ramos; Moraes,Claudia Trigo Pedroso; Oliveira,Danielle Bruna Leal; Lima,Hildener Nogueira; Vieira,Sandra Elisabete; Botosso,Viviane Fongaro; Zanotto,Paolo Marinho de Andrade; Stewien,Klaus Eberhard. |
| The Human Respiratory Syncytial Virus (HRSV), isolated in 1955, is the main cause of hospitalization of babies and infants with respiratory illness. Several studies have been conducted worldwide aiming the development of a safe and effective vaccine against HRSV. The G2 region of glycoprotein G is used as genotyping default. In the present study, we performed a phylogenetic analysis of G protein and a comparative study between G2 region and ectodomain of attachment glycoprotein. Fifty-three nasal swab samples from children less than 5 years old and presenting symptoms of acute respiratory illness, assisted at the University Hospital (UH) of University of Sao Paulo (USP) in 2004, were submitted to sequencing by PCR and compared with GenBank sequences. We... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human Respiratory Syncytial Virus; G Glycoprotein; G2 Region; Phylogenetic Analysis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300005 |
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| Bosso,Patrícia Alves Ramos; Candeias,João Manuel Grisi; Paduan,Karina dos Santos; Ricchetti,Sandra Mara Queiroz; Miranda,Antero Frederico Macedo de; Rugolo,Lígia Maria Suppo de Souza; Durigon,Edison Luiz; Ventura,Armando Morais. |
| Human Respiratory Syncytial Virus (HRSV) is recognized as the single most important pathogen responsible for acute viral infection of the lower respiratory tract in young children. In this study, 84 samples from children under two years of age presenting acute respiratory symptoms were obtained from September 2000 to November 2001. Analysis by immunofluorescence and reverse-transcription followed by PCR, revealed that 18% (15/84) of all the samples were positive for HRSV, in which 80% (12/15) of the cases was observed in children under six months of age and the subgroups A and B co-circulated in the studied period. These are the first data obtained for Botucatu city and seasonality is evident by the higher circulation of the virus between May and July. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human Respiratory Syncytial Virus (HRSV); RT-PCR; Immunofluorescence; Seasonality. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300015 |
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| Kawamoto,Adélia Hiroko Nagamori; Mancini,Dalva Assunção Portari; Pereira,Luiz Eloy; Cianciarullo,Aurora Marques; Cruz,Aurea Silveira; Dias,Andrea Luppi Fernandes; Mendonça,Rita Maria Zucatelli; Pinto,José Ricardo; Durigon,Edison Luiz. |
| Birds are the most important reservoirs of the influenza virus. Its maintenance in its natural hosts, including man, allows the influenza virus to reassorts its strains. The recent report of an avian influenza A (H5N1) virus in humans, was in a child with fatal respiratory illness in China, 1997. The current study was conducted to elucidate the transportation of the influenza by birds that migrate, annually, through the both Northern and Southern hemispheres, with special attention paid to the Vireo olivaceus [Juruviara(BR) or Red-eyed vireo(USA)] species, which travels from the USA to Brazil, and vice versa, and the Elaenia mesoleuca [Tuque(BR) or (USA)] species that flies over the entire Southern Hemisphere. There are two species of birds, which breed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Migrating birds; Brazilian avian influenza virus. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100017 |
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| Toyoshima,Marcos Tadashi Kakitani; Keller,Lilian Walsh; Barbosa,Maria Luisa; Durigon,Edison Luiz. |
| Papular-purpuric "gloves and socks" syndrome (PPGSS) is a novel, rare, self-limiting dermatosis caused by human parvovirus B19. It consists of pruritic edema and erythema of the hands and feet in a gloves-and-socks distribution, and it is associated with oral lesions and fever. We present a case of PPGSS in a 22-year-old Brazilian woman. Clinical and laboratory evaluation, including serological tests, PCR and gene sequencing, confirmed the presence of human parvovirus B19. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Dermatosis; Gloves and socks; Parvovirus B19; PCR; Sequencing. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000100013 |
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| Gardinassi,Luiz Gustavo; Simas,Paulo Vitor Marques; Salomão,João Batista; Durigon,Edison Luiz; Trevisan,Dirce Maria Zanetta; Cordeiro,José Antonio; Lacerda,Mauricio Nogueira; Rahal,Paula; Souza,Fátima Pereira de. |
| Viruses are the major cause of lower respiratory tract infections in childhood and the main viruses involved are Human Respiratory Syncytial Virus (HRSV), Human Metapneumovirus (HMPV), Influenzavirus A and B (FLUA and FLUB), Human Parainfluenza Virus 1, 2 and 3 (HPIV1, 2 and 3) and Human Rhinovirus (HRV). The purposes of this study were to detect respiratory viruses in hospitalized children younger than six years and identify the influence of temperature and relative air humidity on the detected viruses. Samples of nasopharyngeal washes were collected from hospitalized children between May/2004 and September/2005. Methods of viral detection were RT-PCR, PCR and HRV amplicons were confirmed by hybridization. Results showed 54% (148/272) of viral positivity.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Viral Respiratory Infections; HRSV; HRV; Temperature; Relative Air Humidity. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100011 |
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| Vaz,Frederico Fontanelli; Serafini,Patrícia Pereira; Locatelli-Dittrich,Rosangela; Meurer,Rafael; Durigon,Edison Luiz; Araújo,Jansen de; Thomazelli,Luciano Matsumiya; Ometto,Tatiana; Sipinski,Elenise Angelotti Bastos; Sezerban,Rafael Meirelles; Abbud,Maria Cecília; Raso,Tânia Freitas. |
| ABSTRACT The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis. Swab samples were collected from 58 parrots for C. psittaci detection by PCR and from 50... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian influenza virus; Chlamydia psittaci; Microbiology; Newcastle disease; West Nile virus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400747 |
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| Silva,Valéria N.; Durigon,Edison Luiz; Pires,Maria de Fátima Costa; Lourenço,Alexandre; Faria,Maria Jacinta de; Corrêa,Benedito. |
| Two strains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRRL 5940, were studied over a period of 44 days, in order to detect the presence of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids electrophoresis. Only the toxigenic strain contained VLPs, presenting three-segmented dsRNA. An increase in VLPs number was observed during the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycovirus; Double-stranded RNA; Virus-like particles; Aspergillus flavus. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100013 |
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| Registros recuperados: 10 | |
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