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Evaluation of protein extraction methods for enhanced proteomic analysis of tomato leaves and roots Anais da ABC (AABC)
VILHENA,MILCA B.; FRANCO,MÔNICA R.; SCHMIDT,DAIANA; CARVALHO,GISELLE; AZEVEDO,RICARDO A..
Proteomics is an outstanding area in science whose increasing application has advanced to distinct purposes. A crucial aspect to achieve a good proteome resolution is the establishment of a methodology that results in the best quality and wide range representation of total proteins. Another important aspect is that in many studies, limited amounts of tissue and total protein in the tissue to be studied are found, making difficult the analysis. In order to test different parameters, combinations using minimum amount of tissue with 4 protocols for protein extraction from tomato (Solanum lycopersicum L.) leaves and roots were evaluated with special attention to their capacity for removing interferents and achieving suitable resolution in bidimensional gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bidimensional electrophoresis; Proteins; Protocol optimization; Tomato.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401853
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