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| Fajardo,Thor V.M.; Eiras,Marcelo; Schenato,Paula G.; Nickel,Osmar; Kuhn,Gilmar B.. |
| As principais espécies de vírus envolvidas na etiologia do enrolamento da folha da videira (Vitis spp.) são Grapevine leafroll-associated virus 1 e 3 (GLRaV-1 e -3). Neste estudo da variabilidade desses vírus, foram amplificados dois fragmentos de DNA (396 bp do GLRaV-1 e 602 bp do GLRaV-3) por RT-PCR, a partir de RNA total extraído de nervuras e pecíolos de videiras infetadas, utilizando-se dois pares de oligonucleotídeos. Os DNAs amplificados foram clonados e reamplificados, a partir dos clones recombinantes, e comparados quanto às diferenças conformacionais das fitas simples desnaturadas (SSCP). Foram observados dois padrões distintos de perfis eletroforéticos para cada vírus, tendo sido seqüenciado pelo menos um clone viral correspondente a cada... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Videira; Vitis; GLRaV-1; GLRaV-3; SSCP. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200013 |
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| Eiras,Marcelo; Targon,Maria Luisa P.N.; Fajardo,Thor V.M.; Flores,Ricardo; Kitajima,Elliot W.. |
| Viroids, non-protein-coding small (246-401 nt) circular single-stranded RNAs with autonomous replication, are currently classified into two families. Within the family Pospiviroidae, Citrus exocortis viroid (CEVd) belongs to the genus Pospiviroid while Hop stunt viroid (HSVd) is the single member of the genus Hostuviroid. These pathogens are distributed worldwide and infect a large number of hosts. In Brazil, isolates of CEVd and HSVd have been detected in both citrus and grapevine. To characterize and study the genetic variability of these viroids, total RNA from leaves of grapevine Vitis vinifera 'Cabernet Sauvignon' and V. labrusca 'Niagara Rosada' from Bento Gonçalves, RS, was used as a template for RT-PCR amplification with specific primers for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CEVd; HSVd; Pospiviroid; Hostuviroid; Pospiviroidae. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000500002 |
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| Fajardo,Thor V.M.; Eiras,Marcelo; Santos,Henrique P.; Nickel,Osmar; Kuhn,Gilmar B.. |
| Rupestris stem pitting associated virus (RSPaV) é o agente causal das "caneluras do tronco de Rupestris" da videira (Vitis spp.). Neste trabalho, um isolado de RSPaV, encontrado em videiras cv. Cabernet Franc no Rio Grande do Sul, foi estudado. O vírus foi detectado biologicamente, por enxertia em videira indicadora cv. Rupestris du Lot, em 26,2% das amostras avaliadas. A seqüência parcial do gene da replicase do RSPaV, isolado sul-brasileiro, com 831 nucleotídeos amplificados por RT-PCR e 276 aminoácidos deduzidos, apresentou maior identidade de nucleotídeos (98,1%) e aminoácidos deduzidos (99,6%), com dois isolados norte-americanos. O RSPaV estudado apresentou baixa homologia (37-41%) com outros vírus do gênero Foveavirus. A maioria das mudas de videira... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis; RSPaV; Foveavirus; Caracterização. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000200016 |
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| Nickel,Osmar; Targon,Maria L.P.N.; Fajardo,Thor V.M.; Machado,Marcos A.; Trivilin,Ana P.. |
| The coat protein gene of Apple stem grooving virus (ASGV) was amplified by RT-PCR, cloned, sequenced and subcloned in the expression vector pMal-c2. This plasmid was used to transform Escherichia coli BL21c+ competent cells. The ASGV coat protein (cp) was expressed as a fusion protein containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP fusion protein was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fusion protein gave specific reactions to ASGV from infected apple (Malus domestica) cv. Fuji Irradiada and Chenopodium quinoa at dilutions of up to 1:1,000 and 1:2,000, respectively, in plate trapped ELISA. The ASGVcp/MBP... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Recombinant antigen; Latent virus; ELISA; Malus domestica. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500017 |
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| Dianese,Érico de C.; Ramalho,Eduardo D.; Cerqueira,Daniela M.; Lopes,Daniela B.; Fajardo,Thor V.M.; Ferreira,Marisa A.S.V.; Martins,Cláudia R.F.. |
| Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GLRaV-3; Vitis spp.; Ampelovirus; RNA dependent RNA polymerase. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200012 |
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| Fajardo,Thor V.M.; Dianese,Érico C.; Eiras,Marcelo; Cerqueira,Daniela M.; Lopes,Daniela B.; Ferreira,Marisa A.S.V.; Martins,Cláudia R.F.. |
| Leafroll is an economically important disease affecting grapevines (Vitis spp.). Nine serologically distinct viruses, Grapevine leafroll-associated virus-1 through 9, are associated with this disease. The present study describes the coat protein gene sequence of four GLRaV-3 isolates occurring in the São Francisco River basin, Northeastern Brazil. The viral RNA was extracted from GLRaV-3 ELISA-positive plants and the complete coat protein gene was amplified by RT-PCR. Sequences were generated automatically and compared to the complete coat protein sequence from North American (NY1) and Chinese (Dawanhong Nº2 and SL10) GLRaV-3 isolates. The four studied isolates, named Pet-1 through 4, showed deduced amino acid identities of 98-100% (Pet-1 through 3) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Closteroviridae; Ampelovirus; GLRaV-3. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400008 |
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