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Yersinia pestis detection by loop-mediated isothermal amplification combined with magnetic bead capture of DNA BJM
Feng,Na; Zhou,Yazhou; Fan,Yanxiao; Bi,Yujing; Yang,Ruifu; Zhou,Yusen; Wang,Xiaoyi.
ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23 CFU for pure culture, whereas 2.3 × 104 or 2.3 × 106 CFU for simulated spleen and lung samples. For simulated liver samples, the sensitivity of LAMP was 2.3 × 106 CFU, but PCR was negative at the level of 2.3 × 107 CFU. After simulated spleen and lung samples were treated with magnetic beads, the sensitivity of LAMP or PCR was 2.3 × 103 or 2.3 × 106 CFU, whereas 2.3 × 105 or 2.3 × 107 CFU for magnetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plague; Yersinia pestis; Loop-mediated isothermal amplification; Magnetic beads.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100128
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