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Conserved number of U2 snDNA sites in Piabina argentea, Piabarchus stramineus and two Bryconamericus species (Characidae, Stevardiinae) Neotropical Ichthyology
Piscor,Diovani; Fernandes,Carlos Alexandre; Parise-Maltempi,Patricia P..
ABSTRACT The chromosomal location of 5S rRNA and U2 snRNA genes of Piabina argentea, Piabarchus stramineus and two Bryconamericus species from two different Brazilian river basins were investigated, in order to contribute to the understanding of evolutionary characteristics of these repetitive DNAs in the subfamily Stevardiinae. The diploid chromosome number was 2n = 52 for Bryconamericus cf. iheringii, Bryconamericus turiuba, Piabarchus stramineus and Piabina argentea. The 5S rDNA clusters were located on one chromosome pair in P. stramineus and B. cf. iheringii, and on two pairs in B. turiuba and P. argentea. The U2 snDNA clusters were located on the one pair in all species. Two-color FISH experiments showed that the co-localization between 5S rDNA and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Repetitive DNA; Splicing; 5S rDNA; Chromosome; Diploid number.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100207
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First chromosomal analysis of Gymnorhamphichthys britskii: the remarkable lowest diploid value within the family Rhamphichthyidae (Gymnotiformes) Neotropical Ichthyology
Fernandes,Carlos Alexandre; Aguiar,Allan Kardec Moreira de; Paiz,Leonardo Marcel; Baumgärtner,Lucas; Piscor,Diovani; Margarido,Vladimir Pavan.
ABSTRACT Gymnorhamphichthys britskii is a Neotropical electric fish of family Rhamphichthyidae described from the Paraná-Paraguay system. This study reports the first karyotypic description of G. britskii collected from the upper Paraná river basin, which presented 2n=38 chromosomes, karyotype composed of 14 metacentric, 8 submetacentric, 2 subtelocentric and 14 acrocentric chromosomes, and fundamental number as 62 for both sexes. Heteromorphic sex chromosomes were absent. A single pair of nucleolar organizing regions (NORs) was detected in the submetacentric chromosome pair number 9 by silver staining and confirmed by the 18S rDNA probe. The 5S rDNA was located in a single chromosome pair. Heterochromatic regions were clearly observed in the short arms of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ag-NORs; C-banding; Fish cytogenetics; Karyotype evolution; Repetitive sequences.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000300205
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First cytogenetic record for a species of Otothyropsis Ribeiro, Carvalho & Melo, 2005 (Loricariidae, Hypoptopomatinae) Neotropical Ichthyology
Fernandes,Carlos Alexandre; Bailly,Dayani; Sant'Ana,Débora Regina Almeida; Alves,Diandra Soares.
ABTRACT Hypoptopomatinae is a monophyletic subfamily that includes 147 species, distributed in 20 genera. Otothyropsis is a genus of Hypoptopomatinae, recently described. Here, we provided the first cytogenetic information of Otothyropsis . The specimens were collected from córrego Dourado, a small tributary of rio Iguatemi, which flows into rio Paraná. The specimens of Otothyropsis cf. polyodon were analyzed with respect to diploid number, C-Band and Ag-NOR patterns. The diploid number was 54 chromosomes, distributed in 18 metacentric, 28 submetacentric, and 8 subtelocentric chromosomes, with single Ag-NOR and conspicuous heterochromatic blocks on the short and long arms of the 24th pair of chromosomes. Our study highlights the conservation trend of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ag-NOR; Chromosomal evolution; Freshwater fishes; Heterochromatin constitutive; Pericentric inversions.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100224
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Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti & Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil Genet. Mol. Biol.
Fernandes,Carlos Alexandre; Martins-Santos,Isabel Cristina.
Fluorescence in situ hybridization (FISH) was undertaken in order to determinate the chromosomal distribution pattern of 18S and 5S ribosomal DNAs (rDNA) in four populations of the characid fish Astyanax altiparanae from the upper Paraná river basin, Brazil. The 18S rDNA probe FISH revealed numerical and positional variations among specimens from the Keçaba stream compared to specimens of the other populations studied. In contrast to the variable 18S rDNA distribution pattern, highly stable chromosomal positioning of the 5S rDNA sites was observed in the four A. altiparanae populations. Divergence in the distribution pattern of 18S and 5S rDNA sites is also discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax altiparanae; Fluorescence in situ hybridization (FISH); 18S rDNA; 5S rDNA; Sequential Ag-NOR.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300011
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Relative abundance and reproductive tactics of a Loricariidae species at Saraiva Lagoon, Ilha Grande National Park, MS-PR, Brazil Biota Neotropica
Bailly,Dayani; Batista-Silva,Valéria Flávia; Abelha,Milza Celi Fedatto; Kashiwaqui,Elaine Antoniassi Luiz; Fernandes,Carlos Alexandre; Carvalho,Edson Dias de.
This study aimed to evaluate population abundance patterns and the main events in the reproductive cycle of Loricariichthys platymetopon at Saraiva Lagoon, Ilha Grande National Park, MS-PR, Brazil. Monthly samplings were conducted from October 2004 to September 2005 in three sections of the lagoon: entrance, middle and end, which resulted in the capture of 175 individuals. Spatial and temporal variation in abundance was obtained through CPUE (number of captured individuals/1000 m² of gillnets in 12 hours). The reproductive tactics considered were: duration, time and location of spawning, oocyte diameter and fecundity. The highest relative abundance was recorded in February, in the middle section of the lagoon, and the reproductive period lasted from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Distribution; Oocyte diameter; Fecundity; Reproductive period.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032011000300014
Registros recuperados: 5
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