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	Provedor de dados Genet. Mol. Biol. (2) Autor Fernandes-Matioli,F.M.C. (2) Almeida-Toledo,L.F. (1) Matioli,S.R. (1) Pinto,S.M. (1) Schlenz,E. (1) Tipo do documento Info:eu-repo/semantics/article (2) Ano 2000 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última DNA extraction from sea anemone (Cnidaria: Actiniaria) tissues for molecular analyses Genet. Mol. Biol. Pinto,S.M.; Fernandes-Matioli,F.M.C.; Schlenz,E.. A specific DNA extraction method for sea anemones is described in which extraction of total DNA from eight species of sea anemones and one species of corallimorpharian was achieved by changing the standard extraction protocols. DNA extraction from sea anemone tissue is made more difficult both by the tissue consistency and the presence of symbiotic zooxanthellae. The technique described here is an efficient way to avoid problems of DNA contamination and obtain large amounts of purified and integral DNA which can be used in different kinds of molecular analyses. Tipo: Info:eu-repo/semantics/article Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000300017 Species diversity and geographic distribution of Gymnotus (Pisces: Gymnotiformes) by nuclear (GGAC)n microsatellite analysis Genet. Mol. Biol. Fernandes-Matioli,F.M.C.; Matioli,S.R.; Almeida-Toledo,L.F.. Patterns of amplified DNA fragments flanked by (GGAC)n microsatellites, obtained by single primer amplification reaction (SPAR), from 198 Gymnotus specimens (Pisces: Gymnotiformes) sampled from 8 southeastern Brazilian river basins were analyzed. The species studied were Gymnotus carapo, G. pantherinus, G. inaequilabiatus, and G. sylvius. The indirectly obtained patterns reflected the distribution of simple sequence repeats in the nuclear genome of the specimens. Species-specific patterns of DNA amplification were found and were useful for the analysis of the geographic distribution of Gymnotus species. Monomorphic patterns were found in G. carapo, G. pantherinus, and G. inaequilabiatus. Three polymorphic patterns were found in G. sylvius populations. The... Tipo: Info:eu-repo/semantics/article Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000400016 Registros recuperados: 2 Primeira ... 1 ... Última

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