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Single- and Multispecies Biofilms by Escherichia coli, Staphylococcus aureus, and Salmonella spp. Isolated from Raw Fish and a Fish Processing Unit 65
Frozi,Jesieli Braz; Esper,Luciana Maria Ramires; Franco,Robson Maia.
ABSTRACT: The consumption of fish by the Brazilian population is increasing. However, fish and seafood are highly perishable and can be contaminated with several microorganisms. In addition, the possibility of biofilm formation is a greater cause for concern. In this study, biofilm formation was evaluated in single- and multispecies cultures at 25°C for incubation periods of 0, 4, 8, 24, and 48h in stainless steel coupons (size, 1.0×1.0cm) immersed in tryptic soy broth. The characteristics of the formed biofilms after sanitizing by immersing the coupons in 200ppm sodium hypochlorite solution for 10min were also evaluated under the same experimental conditions but with some modifications. Biofilm structure was evaluated using scanning electron microscopy....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella spp.; Escherichia coli; Staphylococcus aureus; Biofilm; Stainless steel; Fish industry..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000451
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Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese 116
Frozi,Jesieli Braz; Domingues,Josiane Roberto; Esper,Luciana Maria Ramires; Corrêa da Rosa,Joel Maurício; Silva,Ana Luiza Sant’Anna da Costa; Gonzalez,Alice Gonçalves Martins.
Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains (isolated by cattle’s faeces and a reference strain, EDL933), were inoculated into pasteurized milk (102 and 103 cells.mL–1) to prepare the Minas frescal cheese. As control was used uninfected milk. Physicochemical and microbiological analyses were performed to milk and elaborated cheese. The O157:H7 strains were quantified in the stages of cheese processing and during 0, 2, 4, 5, 7, 10 and 15 storage days at 8 °C onto Sorbitol MacConkey Agar supplemented with potassium tellurite and cefixime (CT-SMAC). O157:H7 was not present in the pasteurised milk prior to the artificial inoculation. At the end of the processing the cheese had 10 to 100 times more STEC O157:H7 than the initial inoculum....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cheese; Psychrotrophic bacteria; Foodborne disease.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100108
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