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| Registros recuperados: 25 | |
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| AARAB, S.; ARAKRAK, A.; JAVIER OLLERO, F.; MEGÍAS, M.; GOMES, D. F.; RIBEIRO, R. A.; HUNGRIA, M.. |
| Pseudomonas fluorescens ET76 was isolated from rice rhizosphere in northwestern Morocco. Its draft genome was estimated to be 6,681,652 bp with 5,789 coding sequences (CDSs). Genes encoding for type I to VI secretion systems, PvdQ, proteases, siderophores, hydrogen cyanide synthase, ACC-deaminase, among others, highlight its potential use in biological control of plant pathogens. |
| Tipo: Separatas |
Palavras-chave: Oryza sativa L.; Pseudomonas fluorescens; Arroz.; Rice. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1063357 |
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| BATISTA, J. S. da S.; RODRIGUES, E. P.; TORRES, A. R.; GOMES, D. F.; HUNGRIA, M.. |
| A simbiose entre rizóbios e plantas leguminosas, resultando no processo de fixação biológica de nitrogênio (FBN), caracteriza uma das mais importantes interações planta-microrganismo, devido à sua contribuição ao ciclo global de N. Tal interação é um processo complexo, que ocorre mediante a troca de sinais moleculares entre ambos os simbiontes. No Brasil, estirpes selecionadas de Bradyrhizobium japonicum, cujas taxas de fixação de N2 e eficiência simbiótica são comprovadamente reconhecidas, são recomendadas para a utilização em inoculantes comerciais para a soja. Uma das estirpes utilizadas em inoculantes comerciais é a CPAC 15 (=SEMIA 5079), reconhecida por sua elevada competitividade e capacidade saprofítica. Uma maior compreensão sobre a sinalização... |
| Tipo: Separatas |
Palavras-chave: Microbiologia.. |
| Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/918526 |
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| ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MEGÍAS, M.; OLLERO, F. J.; HUNGRIA, M.. |
| Bactérias conhecidas coletivamente como rizóbios estabelecem simbioses com leguminosas, formando estruturas específicas, os nódulos, onde ocorre o processo de fixação biológica do nitrogênio, de grande importância para a agricultura e para o meio ambiente. O processo de nodulação é regulado por genes denominados nod, noe e nol, que em uma etapa inicial, quando induzidos por flavonoides, sintetizam moléculas de oligossacarídeos lipoquitínicos, conhecidas como fatores Nod ou LCOs. A síntese dos fatores Nod está sob controle do gene regulatório nodD. Identificar e analisar genes envolvidos na regulação da biossíntese dos fatores Nod propicia uma maior compreensão dos mecanismos dessa interação planta/bactéria. No genoma de Rhizobium tropici CIAT 899,... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Rizobio; Gene. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010671 |
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| ORMEÑO-ORRILLO, E.; GOMES, D. F.; CERRO, P. del; VASCONCELOS, A. T. R.; CANCHAYA, C.; ALMEIDA, L. G. P.; MERCANTE, F. M.; JAVIER OLLERO, F.; MEGÍAS, M.; HUNGRIA, M.. |
| Common bean (Phaseolus vulgaris L.) is the most important legume cropped worldwide for food production and its agronomic performance can be greatly improved if the benefits from symbiotic nitrogen fixation are maximized. The legume is known for its high promiscuity in nodulating with several Rhizobium species, but those belonging to the Rhizobium tropici ?group? are the most successful and efficient in fixing nitrogen in tropical acid soils. Rhizobium leucaenae belongs to this group, which is abundant in the Brazilian ?Cerrados? soils and frequently submitted to several environmental stresses. Here we present the first high-quality genome drafts of R. leucaenae, including the type strain CFN 299T and the very efficient strain CPAO 29.8. Our main objective... |
| Tipo: Separatas |
Palavras-chave: Fixação biológica de nitrogênio. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056833 |
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| DELAMUTA, J. R. M.; GOMES, D. F.; RIBEIRO, R. A.; CHUEIRE, L. M. O.; SOUZA, R. C.; ALMEIDA, L. G. P.; VASCONCELOS, A. T. R.; HUNGRIA, M.. |
| CNPSo 1112T is a nitrogen-fixing symbiont of perennial soybean, a tropical legume forage. Its draft genome indicates a large genome with a circular chromosome and 9,554 coding sequences (CDSs). Operons of nodulation, nitrogen fixation, and uptake hydrogenase were present in the symbiotic island, and the genome encompasses several CDSs of stress tolerance. |
| Tipo: Separatas |
Palavras-chave: Fixação de nitrogênio; Nitrogen fixation. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032022 |
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| HELENE, L. C. F.; GOMES, D. F.; DELAMUTA, J. R. M.; RIBEIRO, R. A.; SOUZA, R. C.; ALMEIDA, L. G. P.; VASCONCELOS, A. T. R.; HUNGRIA, M.. |
| SEMIA 690T is a nitrogen-fixing symbiont of Centrosema pubescens, and comprises the recently described species Bradyrhizobium viridifuturi. Its draft genome indicates that it belongs to the Bradyrhizobium elkanii superclade. SEMIA 690T carries two copies of the regulatory nodD gene, and the nod and nif operons resemble those of Bradyrhizobium diazoefficiens. |
| Tipo: Separatas |
Palavras-chave: Fixação de nitrogênio; Nitrogen fixation. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032024 |
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| CERRO, P. del; ROLLA-SANTOS, A. A. P.; VALDERRAMA-FERNANDEZ, R.; GIL-SERRANO, A.; BELLOGÍN, R. A.; GOMES, D. F.; MONTAÑO, F. P.; MEGÍAS, M.; HUNGRIA, M.; JAVIER OLLERO, F.. |
| The establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here... |
| Tipo: Separatas |
Palavras-chave: Fixação de nitrogenio; Nitrogen fixation. |
| Ano: 2016 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1043848 |
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| CERRO, P. del; ROLLA-SANTOS, A. A. P.; GOMES, D. F.; MARKS, B. B.; ESPUNY, M. del R.; RODRÍGUEZ-CARVAJAL, M. A.; SORIA-DÍAZ, M. E.; NAKATANI, A. S.; HUNGRIA, M.; JAVIER OLLERO, F.; MEGÍAS, M.. |
| Transcription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes?five?and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. The three nodD mutants were built by insertion of ? interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative... |
| Tipo: Separatas |
Palavras-chave: Rhizobium; Fixação de nitrogênio; Bacteriologia do solo; Nodulação; Bacteriology; Nitrogen fixation; Nodulation.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1039242 |
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| GOMES, D. F.; BATISTA, J. S. da S.; ROLLA, A. A. P.; SILVA, L. P. da; BLOCH, C.; GALLI-TARASAWA, L. V.; HUNGRIA, M.. |
| Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol... |
| Tipo: Separatas |
Palavras-chave: Two-dimensional proteomics; RT-qPCR.; Bradyrhizobium; Nitrogen fixation; Symbiosis.. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/993458 |
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| TULLIO, L. D.; PAULITSCH, F.; REICHERT, P. R. S.; KLEPA, M. S.; KLEPA, M. S.; PILEGGI, M.; GOMES, D. F.; HUNGRIA, M.; BATISTA, J. S. S.. |
| Rhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution... |
| Tipo: Separatas |
Palavras-chave: Fixação de nitrogênio; Nitrogen fixation. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1045758 |
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| TORRES, A. R.; RODRIGUES, E. P.; JESIANE S. S. BATISTA; GOMES, D. F.; HUNGRIA, M.. |
| This research intended to analyze the expression pattern of proteins in roots of the Brazilian soybean cultivar Conquista when inoculated with Bradyrhizobium japonicum CPAC 15, a strain broadly used in commercial inoculants in Brazil. At ten days after bacterial inoculation, whole-cell proteins were extracted from roots and separated by 2-D gel electrophoresis. Comparative analysis revealed significant changes in the intensity of 37 spots due to the inoculation (17 up-regulated and 20 down-regulated proteins), identified by MALDI-TOF/TOF-TOF. Identified proteins were associated with COG functional categories of information storage and processing, cellular processes and signaling, metabolism, and also in the ?poorly characterized? and ?not in COG?... |
| Tipo: Separatas |
Palavras-chave: Rizóbio. |
| Ano: 2013 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/971977 |
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| GOMES, D. F.; BATISTA, J. S. da S.; SCHIAVON, A. L.; ANDRADE, D. S.; HUNGRIA, M.. |
| Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the... |
| Tipo: Separatas |
Palavras-chave: Fixação de nitrogênio; Nitrogen fixation. |
| Ano: 2012 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/932908 |
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| Registros recuperados: 25 | |
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