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Registros recuperados: 9
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Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus. Repositório Alice
PEREIRA, J. F.; ALMEIDA, A. P. M. M.; COTA, J.; PAMPHILE, J. A.; SILVA, G. F. da; ARAÚJO, E. F. de; GRAMACHO, K. P.; BROMMONSCHENKEL, S. H.; PEREIRA, G. A. G.; QUEIROZ, M. V. de.
Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6?12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new...
Tipo: Separatas Palavras-chave: Moniliophthora perniciosa..
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/946075
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Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus. Repositório Alice
PEREIRA, J. F.; ALMEIDA, A. P. M. M.; COTA, J.; PAMPHILE, J. A.; SILVA, G. F. da; ARAUJO, E. F. de; GRAMACHO, K. P.; BROMMONSCHENKEL, S. H.; PEREIRA, G. A. G.; QUEIROZ, M. V. de.
Tipo: Artigo de periódico Palavras-chave: Doença; Fungo; Genética; Planta..
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/976811
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Development of microsatelllte markers for the genetic analysis of Crinipellis perniciosa. Repositório Alice
GRAMACHO, K. P.; RISTERUCCI, A. M.; LANAUD, C.; BAHIA, C.; LOPES, U. V.; GESTEIRA, A. da S.; ARAÚJO, M. R..
Witches' broom disease of cacao (Theobroma cacao L.), caused by Crinipellis perniciosa (Stahel) Singer, is the most importam disease of cacao in the cacao growing areas of South America and Caribbean Tslands. Very little is known about the genetic biology of the pathogen population, and this infonnation is very importam to the host improvement programs and deployment of resistant planting material. A collaborative America Countries intemational program was initiated to identify and describe the genetic diversity of Crinipellis perniciosa in South America. Microsatellites (SSR) constitute highly infonnative genetic markers for popuJation genetic studies due to their co-dominant and multiallelic nature and distribution in the genome. SSR primers were...
Tipo: Artigo de periódico Palavras-chave: Cacau; Doença de planta; Theobroma Cacao; Vassoura de Bruxa; Moniliophthora perniciosa.
Ano: 2005 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/981168
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Encrypted antimicrobial peptides from plant proteins. Repositório Alice
RAMADA, M. H. S.; BRAND, G. D.; ABRÃO, F. Y.; OLIVEIRA, M.; CARDOZO FILHO, J. L.; GALBIERI, R.; GRAMACHO, K. P.; PRATES, M. V.; BLOCH JUNIOR, C..
Tipo: Separatas Palavras-chave: Antibiotics; Peptides; Thermodynamics.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1084036
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First microsatellite markers developed from cupuassu ESTs: application in diversity analysis and cross-species transferability to cacao. Repositório Alice
SANTOS, L. F. dos; FREGAPANI, R. M.; FALCAO, L. L.; TOGAWA, R. C.; COSTA, M. M. do C.; LOPES, U. V.; GRAMACHO, K. P.; ALVES, R. M.; MICHELI, F.; MARCELLINO, L. H..
The cupuassu tree (Theobroma grandiflorum) (Willd. ex Spreng.) Schum. is a fruitful species from the Amazon with great economical potential, due to the multiple uses of its fruit´s pulp and seeds in the food and cosmetic industries, including the production of cupulate, an alternative to chocolate. In order to support the cupuassu breeding program and to select plants presenting both pulp/seed quality and fungal disease resistance, SSRs from Next Generation Sequencing ESTs were obtained and used in diversity analysis. From 8,330 ESTs, 1,517 contained one or more SSRs (1,899 SSRs identified). The most abundant motifs identified in the EST-SSRs were hepta- and trinucleotides, and they were found with a minimum and maximum of 2 and 19 repeats, respectively....
Tipo: Separatas Palavras-chave: Melhoramento genético.; Cacau; Cupuaçu; Fruta Tropical..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1042190
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Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). Repositório Alice
SILVA, R. A. da; SOUZA, G.; LEMOS, L. S. L.; LOPES, U. V.; PATROCÌNIO, N. G. R. B.; ALVES, R. M.; MARCELLINO, L. H.; CLEMENT, D.; MICHELI, F.; GRAMACHO, K. P..
Tipo: Separatas Palavras-chave: Theobroma Cacao.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1069765
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Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). Repositório Alice
SILVA, R. A. da; SOUZA, G.; LEMOS, L. S. L.; LOPES, U. V.; PATROCÌNIO, N. G. R. B.; ALVES, R. M.; MARCELLINO, L. H.; CLEMENT, D.; MICHELI, F.; GRAMACHO, K. P..
The genus Theobroma comprises several trees species native to the Amazon. Theobroma cacao L. plays a key economic role mainly in the chocolate industry. Both cultivated and wild forms are described within the genus. Variations in genome size and chromosome number have been used for prediction purposes including the frequency of interspecific hybridization or inference about evolutionary relationships. In this study, the nuclear DNA content, karyotype and genetic diversity using functional microsatellites (EST-SSR) of seven Theobroma species were characterized. The nuclear content of DNA for all analyzed Theobroma species was 1C = ~ 0.46 pg. These species presented 2n = 20 with small chromosomes and only one pair of terminal heterochromatic bands positively...
Tipo: Artigo de periódico Palavras-chave: Theobroma Cacao; Theobroma.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123321
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High-affinity copper transport and Snq2 export permease of Saccharomyces cerevisiae modulate cytotoxicity of PR-10 from Theobroma cacao. Repositório Alice
PUNGARTNIK, C.; SILVA, A. C. da; MELO, S. A. de; GRAMACHO, K. P.; CASCARDO, J. C. de M.; BRENDEL, M.; MICHELI, F.; GESTEIRA, A. da S..
A pathogenesis-related (PR) protein from Theobroma cacao (TcPR-10) was identified from a cacao Moniliophthora perniciosa interaction cDNA library. Nucleotide and amino acid sequences showed homology with other PR-10 proteins having P loop motif and Betv1 domain. Recombinant TcPR-10 showed in vitro and in vivo ribonuclease activity, and antifungal activity against the basidiomycete cacao pathogen M. perniciosa and the yeast Saccharomyces cerevisiae. Fluorescein isothiocyanate-labeled TcPR-10 was internalized by M. perniciosa hyphae and S. cerevisiae cells and inhibited growth of both fungi. Energy and temperature-dependent internalization of the TcPR-10 suggested an active importation into the fungal cells. Chronical exposure to TcPR-10 of 29 yeast mutants...
Tipo: Artigo de periódico Palavras-chave: Cacau; Doença de Planta; Patógeno; Theobroma Cacao.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/654781
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Unraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis. Repositório Alice
SOARES, V. L. F.; RODRIGUES, S. M.; OLIVEIRA, T. M. de; QUEIROZ, T. O. de; LIMA, L. S.; HORA-JÚNIOR, B. T.; GRAMACHO, K. P.; MICHELI, F.; CASCARDO, J. C. M.; OTONI, W. C.; GESTEIRA, A. S.; COSTA, M. G. C..
The tropical tree Bixa orellana L. produces a range of secondary metabolites which biochemical and molecular biosynthesis basis are not well understood. In this work we have characterized a set of ESTs from a nonnormalized cDNA library of B. orellana seeds to obtain information about the main developmental and metabolic processes taking place in developing seeds and their associated genes. After sequencing a set of randomly selected clones, most of the sequences were assigned with putative functions based on similarity, GO annotations and protein domains. The most abundant transcripts encoded proteins associated with cell wall (prolyl 4-hydroxylase), fatty acid (acyl carrier protein), and hormone/flavonoid (2OG-Fe oxygenase) synthesis, germination (MADS...
Tipo: Separatas Palavras-chave: Apocarotenoids; Bixa orellana L; Carotenoid-cleavage dioxygenase; O-methyltransferase; Bixin; Sequencia genetica; Caroteno; Pigmento; Urucum.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/858658
Registros recuperados: 9
Primeira ... 1 ... Última
 

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