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Compte rendu des premières expérimentations de fécondation artificielle chez l'ombrine, Sciaenops ocellatus : études préliminaires de la maturation finale des ovocytes et de la qualité du sperme 5
Gardes, Lionel; Buchet, Vincent; Villanove, Philippe; Fauvel, Christian.
Artificial insemination mastery in red drum, Sciaenops ocellatus, requires a first experimental step wOOng at getting basic biological data. Regarding males, a relation between optical density of the solution (OD) measured by spectrophotometry and sperm concentration (SC) has been established : OD O,161*SC + 0,119. Mean sperm concentration measured is 4,36.10 10 spz.mL-1. This study shows an influence of the solution of dilution on spermatozoa motility and its evolution during short term preservation. As far as females are concemed, the follow-up of final maturation through the observation of samples taken during reguIar strippings, seems to he a good technique to limit overripening and spontaneous liheration of ovules. Some artificial insemination trials...
Tipo: Text Palavras-chave: Fécondation artificielle; Concentration spermatique; Motilité du sperme; Ovogenèse; Ovulation; Sur maturation; Ombrine; Sciaenops ocellatus.; Artificial insemination; Sperm concentration; Sperm motility; Oogenesis; Ovulation; Overripening; Red drum.; Sciaenops ocella/us.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00366/47755/47801.pdf
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Géodiversité sous-marine de la Nouvelle-Calédonie. 2ème partie 5
Collot, Julien; Patriat, Martin; Rouillard, Pierrick; Samadi, Sarah; Gardes, Lionel; Pelletier, Bernard; Loubersac, Lionel.
This article of the number 10 of Tai Kona is the natural complement to the article appeared under the number 8, dedicated to the submarine geodiversity of the New Caledonian EEZ. The first article notably put the bases of knowledge on the history, the origins and the specificities of the geodiversity of the maritime spaces of the New Caledonian EEZ. The present article focuses on four of the major stakes which are put: 1/ the scientific ones relative to an exceptional submarine geologic context, 2 /the economic ones connected to such a favorable geologic context and to mineral resources exploitation perspectives which are no more domain of the utopia, 3/ the major environmental ones with the existence of a semi deep and deep major biodiversity and 4/ the...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00209/32013/30443.pdf
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Induced spawning of red drum, Sciaenops ocellatus: use of multivariate and univariate analysis methods in the search for side effects of LH-RHa treatments and ovarian development state upon spawn quality 5
Gardes, Lionel; Villanove, Philippe; Buchet, Vincent; Fauvel, Christian.
The present work examines the usefulness of the combination of multivariate analyses and further one-way analysis of variance in the search for links between causes and effects in heterologous stimulation of spawning in fish. Stimulations with both 20 and 50 mu g . kg(-1) doses of [D Trp(6)] luteinizing hormone-releasing hormone analogue (LH-RHa) were effective in inducing and synchronizing spawning of red drum, Sciaenops ocellatus. Oocyte development monitoring using ovarian biopsy allowed treatment injections at different stages of maturation. The influence at stimulation time of female oocyte development state upon further development was shown. An interesting potential of second stimulation, leading to high quality spawns, was established and factors...
Tipo: Text Palavras-chave: Pisciculture; Induction de la ponte; LH-RH analogues; Analyse multivariée; Qualité des oeufs; Développement ovocytaire; Ombrine; Sciaenops ocellatus; Fish culture; Induced breeding; LH-RH analogues; Multivariate analysis; Egg quality; Oocyte development; Red drum; Sciaenops ocellatus.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00187/29875/28361.pdf
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L'induction hormonale de la ponte à l'aide de la LH-RH chez l' ombrine, Sciaenops ocellatus. Mise en évidence des conditions optimales pour la production d'oeufs et de larves de qualité 5
Gardes, Lionel.
20 and 50 µg.kg·1 doses of [D Trp6] LH-RHa analogue proved to be effective in inducing and synchronising spawns in red drum, Sciaenops ocellatus, in the French West Indies tropical conditions. The study of oocyte development through steady ovarian biopsies allowed to carry out injections at different stages. The influence of female ovarian development state at stimulation time on further development is shown. Phase C of germinal vesicle migration stage was identified as an optimum for successful stimulations. An interesting potential of successive stimulations leading to good quality spawns is established and the factors of success identified. The present work shows the  nterest of combined use of multivariate analyses and one way analyse of variance for...
Tipo: Text Palavras-chave: Induction de ponte; LH-RH analogues; Analyse mutivariée; Qualité des oeufs; Développement ovarien; Ombrine; Sciaenops ocellatus; Induced breeding; LH-RH analogues; Mutivariate analysis; Egg quakity; Ovarian development; Red drum; Sciaenops ocellatus.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00425/53672/54512.pdf
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