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Registros recuperados: 6
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Does Cauliflower mosaic virus “sense” the presence of its aphid vector? Inra
Martiniere, A.; Gargani, D.; Blanc, S.; Drucker, M..
Transmission of Cauliflower mosaic virus (CaMV) by aphids depends on the presence of viral electron-lucent inclusion bodies (EL) in infected plant cells. EL contain the aphid transmission factor, the viral protein P2, and the viral protein P3. When EL do not form, no transmission occurs even when infected cells contain functional P2 (Khelifa et al., 2007, J Gen Virol 88, 2872-2880). Thus, EL are structures specialised in transmission, hence our interest to study their formation and functions. We detected that stress induces import of apparently soluble tubulin into EL. FRAP experiments indicated a high turnover rate of EL-contained tubulin. In aphid transmission experiments, we found that aphids fed on stressed infected leaves transmitted CaMV better than...
Tipo: Conference Paper Palavras-chave: CAULIFLOWER MOSAIC VIRUS .
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010404c2821&uri=/notices/prodinra1/2010/05/
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Exocytosis and protein secretion in Trypanosoma Inra
Geiger, A.; Hirtz, C.; Becue, T.; Bellard, E.; Centeno, D.; Gargani, D.; Rossignol, M.; Cuny, G.; Peltier, J.B..
Background: Human African trypanosomiasis is a lethal disease caused by the extracellular parasite Trypanosoma brucei. The proteins secreted by T. brucei inhibit the maturation of dendritic cells and their ability to induce lymphocytic allogenic responses. To better understand the pathogenic process, we combined different approaches to characterize these secreted proteins.Results: Overall, 444 proteins were identified using mass spectrometry, the largest parasite secretome described to date. Functional analysis of these proteins revealed a strong bias toward folding and degradation processes and to a lesser extent toward nucleotide metabolism. These features were shared by different strains of T. brucei, but distinguished the secretome from published T....
Tipo: Journal Article Palavras-chave: TRYPANOSOME; SPECTROMETRIE DE MASSE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20106a992200&uri=/notices/prodinra1/2010/08/
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La face cachée du canal commun des stylets maxillaires de puceron Inra
Uzest-Bonhomme, M.; Gargani, D.; Blanc, S..
La grande majorité des virus de plante utilise un vecteur pour passer d’un hôte à un autre, et lespucerons sont de loin les vecteurs qui transmettent le plus de virus. La transmission non-circulante est la stratégie majoritaire : les virus acquis sur une plante infectée ne sont pasinternalisés dans l’organisme de l’insecte vecteur, mais sont retenus au niveau de ses piècesbuccales, d’où ils pourront être relargués lors de nouvelles piqûres, initiant ainsi l’infection denouvelles plantes hôte.Nous avons utilisé le modèle Cauliflower mosaic virus (CaMV)-puceron pour caractériser lessites d’attachement d’un virus non-circulant dans les stylets de son insecte vecteur. Nous avonsdéveloppé un système d’interaction in vitro, sur les stylets du puceron disséqué,...
Tipo: Conference Paper Palavras-chave: STYLET MAXILLAIRE; CAULIFLOWER MOSAIC VIRUS; CAMV; TRANSMISSION NON CIRCULANTE; PUCERON.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009b7d78be9&uri=/notices/prodinra1/2010/05/
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Structural analysis of cassiicolin, a host-selective protein toxin from Corynespora cassiicola Inra
Barthe, P.; Pujade-Renaud, V.; Breton, F.; Gargani, D.; Thai, R.; Roumestand, C.; De Lamotte, F..
Cassiicolin is a host-selective toxin (HST) produced by the fungus Corynespora cassiicola (strain CCP). It is responsible for the Corynespora leaf fall (CLF) disease, which is among the main pathologies affecting rubber tree (Hevea brasiliensis). Working on purified cassiicolin and using electron microscopy, we have demonstrated that this 27-residue O-glycosylated protein is able to induce cellular damages identical to those induced by the fungus on rubber tree leaves and displays the same host selectivity. The solution structure and disulfide pairing of cassiicolin have been determined using NMR spectroscopy and simulated annealing calculations. Cassiicolin appears to have an original structure with a prolate ellipsoid shape. It adopts an over-all fold...
Tipo: Journal Article Palavras-chave: STRUCTURE 3D HST; NMR STRUCTURE; POST-TRANSLATIONAL MODIFICATIONS; PATHOGENICITY; HOST SELECTIVE TOXIN.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008998eec19&uri=/notices/prodinra1/2008/08/
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The "acrostyle": a newly described anatomical structure in aphid stylets Inra
Uzest, M.; Gargani, D.; Dombrovsky, A.; Cazevieille, C.; Blanc, S.; Cot, D..
The recent demonstration that a plant virus could be retained on protein receptors located exclusively in a small area inside the common duct at the tip of aphid maxillary stylets indicated the possible existence of a distinct anatomical structure at this level. Since no distinct feature within the common duct of any aphid species has ever been reported in the literature, we first carefully re-examined the distal extremity of the maxillary stylets of Acyrthosiphon pisum using transmission- and scanning-electron microscopy. Here, we describe an area of the cuticle surface displaying a different structure that is limited to a “band” paving the bottom of the common duct in each opposing maxillary stylet. This band starts at the very distal extremity, adopts...
Tipo: Journal Article Palavras-chave: APHID; MAXILLARY STYLETS; TRANSMISSION ELECTRON MICROSCOPY; SCANNING ELECTRON MICROSCOPY; CUTICULAR PROTEIN; VIRUS TRANSMISSION.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20103afec26f&uri=/notices/prodinra1/2010/08/
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Une protéine clé pour la transmission d'un virus de plante à la pointe des stylets de l'insecte vecteur Inra
Blanc, S.; Uzest, M.; Candresse, T.; Drucker, M.; Fereres, A.; Gargani, D.; Garzo, E.; Hébrard, E..
Du fait de l’immobilité de leurs hôtes, l’immense majorité des virus de planteutilisent des vecteurs spécifiques pour passer d’un hôte à un autre. Ces « véhiculesde transport » sont principalement des arthropodes et en grande majoritédes pucerons, qui sont des insectes de type piqueur-suceur [1]. Pour les interactionsvirus-vecteur, la stratégie la plus communément utilisée par les virus deplante est la transmission dite non circulante, où les particules virales prélevéeslors d’un repas dans les cellules infectées seront retenues au niveau de sitesd’attachement dans les pièces buccales antérieures de l’insecte sans effectuer depassage à l’intérieur de son organisme. Ces particules virales seront ensuiterelarguées de ces sites d’attachement lors de piqûres...
Tipo: Journal Article Palavras-chave: RECEPTEUR PROTEIQUE; STYLET; INTERACTION IN VITRO CAULIFLOWER MOSAIC VIRUS.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008b214d5fc&uri=/notices/prodinra1/2008/05/
Registros recuperados: 6
Primeira ... 1 ... Última
 

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