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| Martiniere, A.; Gargani, D.; Blanc, S.; Drucker, M.. |
| Transmission of Cauliflower mosaic virus (CaMV) by aphids depends on the presence of viral electron-lucent inclusion bodies (EL) in infected plant cells. EL contain the aphid transmission factor, the viral protein P2, and the viral protein P3. When EL do not form, no transmission occurs even when infected cells contain functional P2 (Khelifa et al., 2007, J Gen Virol 88, 2872-2880). Thus, EL are structures specialised in transmission, hence our interest to study their formation and functions. We detected that stress induces import of apparently soluble tubulin into EL. FRAP experiments indicated a high turnover rate of EL-contained tubulin. In aphid transmission experiments, we found that aphids fed on stressed infected leaves transmitted CaMV better than... |
| Tipo: Conference Paper |
Palavras-chave: CAULIFLOWER MOSAIC VIRUS . |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010404c2821&uri=/notices/prodinra1/2010/05/ |
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| Geiger, A.; Hirtz, C.; Becue, T.; Bellard, E.; Centeno, D.; Gargani, D.; Rossignol, M.; Cuny, G.; Peltier, J.B.. |
| Background: Human African trypanosomiasis is a lethal disease caused by the extracellular parasite Trypanosoma brucei. The proteins secreted by T. brucei inhibit the maturation of dendritic cells and their ability to induce lymphocytic allogenic responses. To better understand the pathogenic process, we combined different approaches to characterize these secreted proteins.Results: Overall, 444 proteins were identified using mass spectrometry, the largest parasite secretome described to date. Functional analysis of these proteins revealed a strong bias toward folding and degradation processes and to a lesser extent toward nucleotide metabolism. These features were shared by different strains of T. brucei, but distinguished the secretome from published T.... |
| Tipo: Journal Article |
Palavras-chave: TRYPANOSOME; SPECTROMETRIE DE MASSE. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20106a992200&uri=/notices/prodinra1/2010/08/ |
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| Blanc, S.; Uzest, M.; Candresse, T.; Drucker, M.; Fereres, A.; Gargani, D.; Garzo, E.; Hébrard, E.. |
| Du fait de l’immobilité de leurs hôtes, l’immense majorité des virus de planteutilisent des vecteurs spécifiques pour passer d’un hôte à un autre. Ces « véhiculesde transport » sont principalement des arthropodes et en grande majoritédes pucerons, qui sont des insectes de type piqueur-suceur [1]. Pour les interactionsvirus-vecteur, la stratégie la plus communément utilisée par les virus deplante est la transmission dite non circulante, où les particules virales prélevéeslors d’un repas dans les cellules infectées seront retenues au niveau de sitesd’attachement dans les pièces buccales antérieures de l’insecte sans effectuer depassage à l’intérieur de son organisme. Ces particules virales seront ensuiterelarguées de ces sites d’attachement lors de piqûres... |
| Tipo: Journal Article |
Palavras-chave: RECEPTEUR PROTEIQUE; STYLET; INTERACTION IN VITRO CAULIFLOWER MOSAIC VIRUS. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008b214d5fc&uri=/notices/prodinra1/2008/05/ |
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