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Efeito do tipo de envase, flatpacks ou palhetas, sobre a criopreservação da fração rica do sêmen asinino 45
Sales,A.L.R.; Gonczarowska,A.T.; Carvalho,L.E.; Palhares,M.S.; Rossi,R.; Silva Filho,J.M..
Com o objetivo de se estudar o efeito do tipo de envase sobre a criopreservação do sêmen asinino, coletado de forma fracionada, utilizou-se o sêmen de cinco jumentos da raça Pêga, submetido a um protocolo de congelamento envolvendo um diluidor e duas formas de envase, flatpacks ou palhetas de 0,55mL, tendo como ponto final a avaliação dos parâmetros seminais in vitro após o descongelamento do sêmen. Os resultados de motilidade espermática para as amostras envasadas em flatpacks ou em palhetas foram de 24,67% e 35,34%, no momento do descongelamento; de 13,39% e 25,83%, 60 minutos após o descongelamento; e de 7,86% e 13,33%, aos 120 minutos após o descongelamento, respectivamente. Já os valores para o vigor espermático foram de 2,55 e 3,33, no momento do...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jumento; Coleta fracionada; Sêmen congelado; Flatpacks; Palhetas.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401016
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Effect of month of conception on fertility of mares inseminated with jackass semen 45
Rossi,R.; Gonczarowska,A.T.; Palhares,M.S.; Boeta,A.M.; Resende,Y.F.; Ribeiro,E.A.; Silva Filho,J.M..
Fertility obtained by cross-breeding mares (Equus caballus) with jackasses (Equus asinus) was evaluated. Two extenders, containing skim milk-glucose or egg yolk-glycine were used to study the fertility of mares inseminated with diluted jackass semen (T1 and T2) or diluted and cooled semen at 5°C for 12 hours (T3 and T4). A total of 272 cycles of 208 mares of undefined breeds were evaluated, being uniformly distributed between groups. The cycles were controlled by transrectal palpation and teasing, and mares were inseminated every Tuesday, Thursday and Saturday (three times/week), from the detection of a follicle with 3.0 to 3.5cm diameter in one of the ovaries until ovulation. Pregnancy was detected using transrectal palpation, teasing and ultrasound exams...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fertility; Pregnancy loss; Intra and interspecific cross-breeding; Cooled semen.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000100057
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Physical and morphological characteristics of the first three jets of Pêga jackasses sperm-rich fraction 45
Carvalho,L.E.; Silva Filho,J.M.; Palhares,M.S.; Sales,A.L.R.; Gonczarowska,A.T.; Oliveira,H.N.; Resende,M.; Rossi,R..
ABSTRACT The first three jets of the sperm-rich fraction of Pêga jackasses were collected and assessed separately. Five fertile Pêga jackasses were used as semen donors and underwent fractionated semen collection, using an open model artificial vagina. The first three jets of the semen were collected separately and assessed for volume, sperm motility, vigor, concentration/mL of semen, and sperm morphology. These characteristics were compared between first, second and third jets and between jackasses. It was observed that the jet volume differed (P<0.05) between jackasses, although it was similar (P>0.05) between first, second and third jets. Sperm motility did not differ (P>0.05) between jets and jackasses. Vigor was similar (P>0.05) between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jackass; Fractionated ejaculate; Jets; Sperm-rich fraction.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400845
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Physical characteristics and fertility of fractionated donkey semen cooled at 5°C 45
Carvalho,L.E.; Silva Filho,J.M.; Palhares,M.S.; Sales,A.L.R.; Gonczarowska,A.T.; Oliveira,H.N.; Resende,M.C.; Neves,M.G.; Madison,R.J..
ABSTRACT The aim of this study was to evaluate the effect of two different extenders (Skimmed Milk Glucose - SMG or Lactose - Egg Yolk - LEY) on physical characteristics and fertility of fractionated donkey semen cooled at 5°C. For this, four Pêga donkeys were used as semen donors. The sperm rich fraction of the ejaculate was diluted preparing insemination doses containing 400 x 106 motile spermatozoa in a volume of 22 mL, cooled to 5°C and stored up to 48 hours in a container proposed by Palhares (1997). Sperm motility and vigor were assessed in fresh semen, after first semen dilution, before insemination, at 24 and 48 hours after storage. For the fertility evaluation, 44 mares were inseminated with semen stored for a period between 12 and 24 hours. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Donkey; Fractionated collection; Cooled semen; Fertility.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100029
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Reproductive biology of Pêga Jackasses: characteristics of sperm-rich fraction collected using the split-ejaculate method 45
Carvalho,L.E.; Silva Filho,J.M.; Palhares,M.S.; Sales,A.L.R.; Gonczarowska,A.T.; Oliveira,H.N.; Resende Jr.,T.; Rossi,R..
ABSTRACT The aim of this study was to evaluate the physical and morphological characteristics of the sperm-rich fraction of jackass semen. To this end, 130 ejaculates from five Pêga jackasses were collected using an open model artificial vagina. The sperm-rich fraction was collected using the split-ejaculate method and assessed for the number of mounts/ejaculate, for physical and morphological characteristics of the semen and number of doses produced/ejaculate. It was observed that all characteristics evaluated differed among the five jackasses, except for the head defect rates. The mean values obtained for the jackass sperm-rich fraction collected were: number of mounts/ejaculate - 1.27; semen volume - 20.21mL; motility - 84.53%; vigor - 4.46; motility...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Jackass; Split-ejaculate method; Sperm-rich fraction.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400832
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