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| De Charleroy, D.; Cannaerts, V.; Augustijn, H.; Grisez, L.; Boon, J.; Ollevier, F.P.. |
| A standardized method was developed for artificially infecting the European eel with <i>Anguillicola crassus</i>, a parasitical swim bladder nematode. For this purpose, an intermediate host, <i>Paracyclops fimbriatus</i>, and a paratenic host, <i>Cyprinus carpio</i>, were used. Parasite prevalence in the artificially infected eels was very high, although there was a large variation in the number of worms per eel. Compared to other methods of artificial infection described in the literature, this method appears to be more efficient ad reliable; there were also more infectious larvae that were recovered and the mortality rate of eels under treatment was lower. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hosts Infestation Invertebrate larvae Methodology Mortality Swim bladder Anguilla anguilla (Linnaeus; 1758) Anguillicola crassus Copepoda [copepods] Cyprinus carpio Nematoda [Nematodes] Paracyclops fimbriatus (Fischer; 1853). |
| Ano: 1990 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3073 |
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| Grisez, L.; Ollevier, F.P.. |
| The different serotyping systems, based on thermostable O antigens, reported for <i>Vibrio anguillarum</i> and <i>V. ordalii</i> were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within <i>V. anguillarum</i> (designated 01 to 016) can be distinguished. Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS. The comparative analysis allowed a complete alignment of the different stereotypes for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pathogens; Serology; Vibrio anguillarum. |
| Ano: 1995 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2970 |
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| Pedersen, K.; Grisez, L.; van Houdt, R.; Tiainen, T.; Ollevier, F.P.; Larsen, J.L.. |
| The present paper summarizes the serotyping scheme of the fish pathogenic bacterium <i>Vibrio anguillarum</i> and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were characterized further by means of LPS profiles, Western blots, and serological reactions. On the basis of the results, it is suggested that the seven new O-serogroups are to be included in the existing serotyping system as serotypes O17-O<sub>2</sub>3. However, the existence of further <i>V. anguillarum</i> strains that were not typable with any of the 23 O-antisera suggested the existence of additional... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial diseases; Fish diseases; Pathogenic bacteria; Pathogens; Serological studies; Serotypes; Strains; Vibrio anguillarum. |
| Ano: 1999 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9525 |
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| Thyssen, A.; Grisez, L.; van Houdt, R.; Ollevier, F.P.. |
| The taxonomic position of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>, the causative agent of fish pasteurellosis, is controversial as this organism has also been described as '<i>Pasteurella piscicida</i>'. To clarify the taxonomic position of the pathogen, a total of 113 <i>P. damselae</i> subsp. <i>piscicida</i> strains and 20 <i>P. damselae</i> subsp. <i>damselae</i> strains, isolated from different geographical areas and from the main affected fish species, were analysed using 129 morphological and biochemical tests, including the commercial API 20E and API CH50 test systems. For comparison, the type strains of other <i>Photobacterium</i> species... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Numerical taxonomy. |
| Ano: 1998 |
URL: http://www.vliz.be/imisdocs/publications/238535.pdf |
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