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| Mercey, R.; Lantier, I.; Maurel, M.C.; Grosclaude, J.; Lantier, F.; Marc, D.. |
| One of the unsolved problems in prion diseases relates to the physiological function of cellular prion protein (PrP), of which a misfolded isoform is the major component of the transmissible spongiform encephalopathies agent. Knowledge of the PrP-binding molecules may help in elucidating its role and understanding the pathological events underlying prion diseases. Because nucleic acids are known to bind PrP, we attempted to identify the preferred RNA sequences that bind to the ovine recombinant PrP. An in vitro selection approach (SELEX) was applied to a pool of 80-nucleotide(nt)-long RNAs containing a randomised 40-nt central region. The most frequently isolated aptamer, RM312, was also the best ligand (20 nM KD value), according to both surface plasmon... |
| Tipo: Journal Article |
Palavras-chave: SEQUENCE; AMPLIFICATION; TRANSCRIPTION. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007b7bf6e45&uri=/notices/prodinra1/2010/10/ |
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| Akimov, V.; Alfinito, E.; Bausells, J.; Benilova, I.; Paramo, I.C.; Errachid, A.; Ferrari, G.; Fumagalli, L.; Gomila, G.; Grosclaude, J.; Hou, Y.; Jaffrezic-Renault, N.; Martelet, C.; Pajot-Augy, E.; Pennetta, C.; Persuy, M.A.; Pla-Roca, M.; Reggiani, L.; Rodriguez-Segui, S.; Ruiz, O.; Salesse, R.; Samitier, J.; Sampietro, M.; Soldatkin, A.P.; Vidic, J.; Villanueva, G.. |
| The animal olfactory system represents the goldstandard of biosensors, due to its ability to identify anddiscriminate thousands of odorant compounds with very lowthresholds. Using olfactory receptors (ORs) as sensing elementsinstead of chemical sensors, biosensors would benefitthe naturally optimized molecular recognition of odorants to develop a new generation of bioelectronic noses. Thepurpose of SPOT-NOSED European project was the developmentof nanobiosensors based on single ORs anchoredbetween nanoelectrodes, to mimic the performances of naturalolfactory system. Nanobiosensors arrays could thenincrease odorant sensitivity or widen the odorant detection |
| Tipo: Journal Article |
Palavras-chave: RECEPTEUR OLFACTIF; NEZ ELECTRONIQUE; SYSTEME OLFACTIF BIOSENSORS; ELECTRONIC NOSE; OLFACTORY RECEPTORS; NANOTECHNOLOGY; ODORANT. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20082ffd84aa&uri=/notices/prodinra1/2010/09/ |
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