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Inorganic nitrogen application affects both taxonomical and predicted functional structure of wheat rhizosphere bacterial communities. Repositório Alice
KAVAMURA, V. N.; HAYAT, R.; CLARK, I. M.; ROSSMANN, M.; MENDES, R.; HIRSCH, P. R.; MAUCHLINE, T. H..
The effects of fertilizer regime on bulk soil microbial communities have been well studied, but this is not the case for the rhizosphere microbiome. The aim of this work was to assess the impact of fertilization regime on wheat rhizosphere microbiome assembly and 16S rRNA gene-predicted functions with soil from the long term Broadbalk experiment at Rothamsted Research. Soil from four N fertilization regimes (organic N, zero N, medium inorganic N and high inorganic N) was sown with seeds of Triticum aestivum cv. Cadenza. 16S rRNA gene amplicon sequencing was performed with the Illumina platform on bulk soil and rhizosphere samples of 4-week-old and flowering plants (10 weeks). Phylogenetic and 16S rRNA gene-predicted functional analyses were performed....
Tipo: Separatas Palavras-chave: Nitrogênio inorgânico; Trigo; Fertilizante nitrogenado; Impacto ambiental; Rizosfera; Wheat; Rhizosphere; Microbiome; Nitrogen fertilizers; Environmental impact; Bacterial communities; Functional diversity; Sustainable agricultural intensification.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105404
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Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. Repositório Alice
KAVAMURA, V. N.; ROBINSON, R. J.; HAYAT, R.; CLARK, I. M.; HUGHES, D.; ROSSMANN, M.; HIRSCH, P. R.; MENDES, R.; MAUCHLINE, T. H..
Abstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the...
Tipo: Artigo de periódico Palavras-chave: Endosphere; Rizosfera; População Microbiana; Manejo do Solo; Trigo; Semente; Rhizosphere; Microbiome; Wheat; Seeds.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412
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