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| PYLRO, V. S.; ROESCH, L. F. W.; ORTEGA, J. M.; AMARAL, A. M. do; TÓTOLA, M. R.; HIRSCH, P. R.; ROSADO, A. S.; GÓES-NETO, A.; SILVA, A. L. da C.; ROSA, C. A.; MORAIS, D. K.; ANDREOTE, F. D.; DUARTE, G. F.; MELO, I. S. de; SELDIN, L.; LAMBAIS, M. R.; HUNGRIA, M.; PEIXOTO, R. S.; KRUGER, R. H.; TSAI, S. M.; AZEVEDO, V.. |
| The Brazilian Microbiome Project (BMP) aims to assemble a Brazilian Metagenomic Consortium/Database. At present, many metagenomic projects underway in Brazil are widely known. Our goal in this initiative is to co-ordinate and standardize these together with new projects to come. It is estimated that Brazil hosts approximately 20 % of the entire world's macroorganism biological diversity. It is 1 of the 17 countries that share nearly 70 % of the world's catalogued animal and plant species, and is recognized as one of the most megadiverse countries. At the end of 2012, Brazil has joined GBIF (Global Biodiversity Information Facility), as associated member, to improve the access to the Brazilian biodiversity data in a free and open way. This was an important... |
| Tipo: Separatas |
Palavras-chave: Diversidade microbiana; Biodiversidade; Microrganismo; Biodiversity. |
| Ano: 2014 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1000621 |
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| KAVAMURA, V. N.; HAYAT, R.; CLARK, I. M.; ROSSMANN, M.; MENDES, R.; HIRSCH, P. R.; MAUCHLINE, T. H.. |
| The effects of fertilizer regime on bulk soil microbial communities have been well studied, but this is not the case for the rhizosphere microbiome. The aim of this work was to assess the impact of fertilization regime on wheat rhizosphere microbiome assembly and 16S rRNA gene-predicted functions with soil from the long term Broadbalk experiment at Rothamsted Research. Soil from four N fertilization regimes (organic N, zero N, medium inorganic N and high inorganic N) was sown with seeds of Triticum aestivum cv. Cadenza. 16S rRNA gene amplicon sequencing was performed with the Illumina platform on bulk soil and rhizosphere samples of 4-week-old and flowering plants (10 weeks). Phylogenetic and 16S rRNA gene-predicted functional analyses were performed.... |
| Tipo: Separatas |
Palavras-chave: Nitrogênio inorgânico; Trigo; Fertilizante nitrogenado; Impacto ambiental; Rizosfera; Wheat; Rhizosphere; Microbiome; Nitrogen fertilizers; Environmental impact; Bacterial communities; Functional diversity; Sustainable agricultural intensification. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105404 |
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| KAVAMURA, V. N.; ROBINSON, R. J.; HAYAT, R.; CLARK, I. M.; HUGHES, D.; ROSSMANN, M.; HIRSCH, P. R.; MENDES, R.; MAUCHLINE, T. H.. |
| Abstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the... |
| Tipo: Artigo de periódico |
Palavras-chave: Endosphere; Rizosfera; População Microbiana; Manejo do Solo; Trigo; Semente; Rhizosphere; Microbiome; Wheat; Seeds. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412 |
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| KAVAMURA, V. N.; CLARK, I.; HIRSCH, P. R.; CAULFIELD, J.; SARRIA, A. F.; ROSSMANN, M.; BIRKETT, M.; MELO, I. S. de; MENDES, R.; MAUCHLINE, T.. |
| During the Green Revolution, wheat breeding developed high-yielding plants with dwarfing genes. This selection led to structural changes in wheat, which could have consequences for the associated microbial communities, however, to date, little is known about these. The main goal of this work was to study rhizosphere bacterial communities from eight hexaploid wheat cultivars grown under field conditions ranging in chronology from Chidham White Chaff, a tall cultivar from 1790, to Crusoe, a semi dwarf cultivar from 2012. Plants were harvested, DNA from rhizosphere soil was extracted and 16S rRNA gene subjected to Illumina amplicon sequencing. Root architecture information and exudate profiles were obtained. The breeding process appears to have influenced... |
| Tipo: Separatas |
Palavras-chave: Wheat; Rhizosphere; Roots. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103550 |
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| KAVAMURA, V. N.; ROBINSON, R. J.; HUGHES, D.; CLARK, I. M.; ROSSMANN, M.; MELO, I. S. de; HIRSCH, P. R.; MENDES, R.; MAUCHLINE, T. H.. |
| Abstract: The development of dwarf wheat cultivars combined with high levels of agrochemical inputs during the green revolution resulted in high yielding cropping systems. However, changes in wheat cultivars were made without considering impacts on plant and soil microbe interactions. We studied the effect of these changes on root traits and on the assembly of rhizosphere bacterial communities by comparing eight wheat cultivars ranging from tall to semi-dwarf plants grown under field conditions. Wheat breeding influenced root diameter and specific root length (SRL). Rhizosphere bacterial communities from tall cultivars were distinct from those associated with semi-dwarf cultivars, with higher differential abundance of Actinobacteria, Bacteroidetes and... |
| Tipo: Artigo de periódico |
Palavras-chave: Trigo; Rizosfera; Melhoramento Genético Vegetal; Microbiologia do Solo; Wheat; Dwarf cultivars; Rhizosphere; Ribosomal RNA; Plant breeding; Microbiology. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123855 |
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