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| Habachi-Houimli,Yosra; Cherif,Mejda; Gharsallah,Charfeddine; Sébéi,Abdennour; Makni,Mohamed; Bouktila,Dhia. |
| Abstract Pyrenophora teres f. maculata is the causal agent of barley spot form net blotch (SFNB), a major stubble-borne disease in many barley-growing areas worldwide. In plants, the Nucleotide-Binding Site-Leucine-Rich Repeat (NBS-LRR) gene family functions in immunity against a variety of pathogens and pests. From a pre-established set of NBS-type resistance gene candidates, we have selected three candidate genes, HvNBS10, HvNBS72 and HvNBS85, to analyze their possible involvement in P. teres f. maculata resistance. The studied genes were mapped on chromosomes 5H and 7H. Expression profiles using qRT-PCR, 48 hours after infection by P. teres. f. maculata, revealed that the transcription of all genes acted in the same direction (down-regulation) in both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spot form net blotch (SFNB); Nucleotide-binding site (NBS); Leucine-rich repeat (LRR); QRT-PCR; Hordeum vulgare. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100425 |
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| Bouktila,Dhia; Khalfallah,Yosra; Habachi-Houimli,Yosra; Mezghani-Khemakhem,Maha; Makni,Mohamed; Makni,Hanem. |
| Proteins containing nucleotide binding sites (NBS) encoded by plant resistance genes play an important role in the response of plants to a wide array of pathogens. In this paper, an in silico search was conducted in order to identify and characterize members of NBS-encoding gene family in the tribe of Triticeae. A final dataset of 199 sequences was obtained by four search methods. Motif analysis confirmed the general structural organization of the NBS domain in cereals, characterized by the presence of the six commonly conserved motifs: P-loop, RNBS-A, Kinase-2, Kinase-3a, RNBS-C and GLPL. We revealed the existence of 11 distinct distribution patterns of these motifs along the NBS domain. Four additional conserved motifs were shown to be significantly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NBS domain; Data mining; Phylogeny; Plant resistance genes; Triticeae. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400017 |
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