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Registros recuperados: 7
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A simplified procedure for terminal restriction fragment length polymorphism analysis of the soil bacterial community to study the effects of pesticides on the soil microflora using 4,6-dinitroorthocresol as a test case Inra
Rousseaux, S.; Hartmann, A.; Rouard, N.; Soulas, G..
We have studied the structural effects of application to the soil of a potentially detrimental herbicide, 4,6-dinitroorthocresol (DNOC) by analysing amplified ribosomal DNA restriction analysis (ARDRA) and terminal restriction fragment length polymorphism (T-RFLP) signatures of 16S rDNA fragments of culturable bacterial communities isolated from diluted soil suspensions. This approach has the potential to reveal changes induced by stressing the soil microflora with DNOC. This paper shows that, whereas only few changes of the ARDRA and T-RFLP profiles result from ageing of the soil, treatment of the soil with DNOC induces major modifications of these profiles. Therefore, for the practical purpose of pesticide registration, ARDRA and T-RFLP analysis...
Tipo: Journal Article Palavras-chave: 4 6-DINITROORTHOCRESOL; COMMUNAUTE MICROBIENNE; RFLP.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400035298104675&uri=/notices/prodinra1/2010/12/
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Diversity of denitrifying microflora and ability to reduce N2O in two soils Inra
Chèneby, D.; Hartmann, A.; Hénault, C.; Topp, E.; Germon, J.C..
 The ozone-depleting gas N2O is an intermediate in denitrification, the biological reduction of NO3 – to the gaseous products N2O and N2 gas. The molar ratio of N2O produced (N2O/N2O+N2) varies temporally and spatially, and in some soils N2O may be the dominant end product of denitrification. The fraction of NO3 –-N emitted as N2O may be due at least in part to the abundance and activity of denitrifying bacteria which possess N2O reductase. In this study, we enumerated NO3 –-reducing and denitrifying bacteria, and compared and contrasted collections of denitrifying bacteria isolated from two agricultural soils, one (Auxonne, soil A) with N2O as the dominant product of denitrification, the other (Châlons, soil C) with N2 gas as the dominant product....
Tipo: Journal Article Palavras-chave: IDENTIFICATION; REDUCTASE; DENITRIFICATION; RFLP.
Ano: 1998 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9900033420073695&uri=/notices/prodinra1/2010/12/
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Final report "PathOrganic. Risks and Recommendations Regarding Human Pathogens in Organic Vegetable Production Chains" Organic Eprints
Hackl, E.; Sessitsch, A.; Arthurson, V.; Baggesen, D.L.; Dalsgaard, A.; Friedel, J.K.; Hartmann, A.; Koller, M.; van Bruggen, A.H.C.; Widmer, F.; Wyss, G.; Zijlstra, C.A..
PathOrganic assesses risks associated with the consumption of fresh and minimally processed vegetables due to the prevalence of bacterial human pathogens (e.g. Salmonella enterica, pathogenic E. coli, Campylobacter spp., Listeria monocytogenes) in organically grown plant produce. The project aims at evaluating whether organic production poses a risk on food safety and addresses the food chain by taking into consideration potential sources of pathogen transmission (e.g. animal manure). In addition, it evaluates whether organic production may reduce the risk of pathogen manifestation. From a European perspective, vegetable-linked outbreaks are not well investigated. Within the PathOrganic project, surveys of organically grown vegetables are carried out in...
Tipo: Report Palavras-chave: Food security; Food quality and human health Vegetables.
Ano: 2012 URL: http://orgprints.org/21197/1/PathOrganic_final_report_OrgEprints.doc
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Occurrence and distribution of endobacteria in the plant-associated mycelium of the ectomycorrhizal fungus Laccaria bicolor S238N Inra
Bertaux, J.; Schmid, M.; Hutzler, P.; Hartmann, A.; Garbaye, J.; Frey-Klett, P..
Fluorescence in situ hybridization, associated with confocal laser scanning microscopy or epifluorescence microscopy with deconvolution system, has allowed the detection of a community of intracellular bacteria in non-axenic samples of the ectomycorrhizal fungus Laccaria bicolor S238N. The endobacteria, mainly alpha -proteobacteria, were present in more than half of the samples, which consisted of ectomycorrhizae, fungal mats and fruit bodies, collected in the glasshouse or in the forest. Acridine orange staining suggests that the endobacteria inhabit both live and dead fungal cells. The role of these endobacteria remains to be clarified.
Tipo: Journal Article Palavras-chave: ECTOMYCORHIZE; LACCARIA BICOLOR; MYCELIUM ENDOBACTERIE; CHAMPIGNON.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20076a4afaf1&uri=/notices/prodinra1/2007/07/
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PathOrganic – Risks and Recommendations Regarding Human Pathogens in Organic Vegetable Production Chains Organic Eprints
Arthurson, V.; Baggesen, D.; Brankatschk, K.; Dalsgaard, A.; Duffy, B.; Fenzl, C.; Friedel, J.K.; Hackl, E.; Hartmann, A.; Hedin, F.; Hofmann, A.; Jäderlund, L.; Jansson, J.; Jensen, A.N.; Koller, M.; Mäder, P.; Rinnofner, T.; Schmid, M.; Storm, C.; van Bruggen, A.H.C.; Widmer, F.; Wyss, G.S.; Zijlstra, C.A.; Sessitsch, A..
PathOrganic assesses risks associated with the consumption of fresh and minimally processed vegetables due to the prevalence of bacterial human pathogens in plant produce. The project evaluates whether organic production poses a risk on food safety, taking into consideration sources of pathogen transmission (e.g. animal manure). The project also explores whether organic versus conventional production practices may reduce the risk of pathogen manifestation. In Europe, vegetable-linked outbreaks are not well investigated. A conceptual model together with novel sampling strategies and specifically adjusted methods provides the basis for large-scale surveys of organically grown plant produce in five European countries. Critical control points are...
Tipo: Conference paper, poster, etc. Palavras-chave: "Organics" in general; Vegetables.
Ano: 2009 URL: http://orgprints.org/14310/1/Arthurson_14310.pdf
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Plant-driven selection of microbes Inra
Hartmann, A.; Schmid, M.; Van Tuinen, D.; Berg, G..
The rhizodeposition of plants dramatically influence the surrounding soil and its microflora. Root exudates have pronounced selective and promoting effects on specific microbial populations which are able to respond with chemotaxis and fast growth responses, such that only a rather small subset of the whole soil microbial diversity is finally colonizing roots successfully. The exudates carbon compounds provide readily available nutrient and energy sources for heterotrophic organisms but also contribute e.g. complexing agents, such as carboxylates, phenols or siderophores for the mobilization and acquisition of rather insoluble minerals. Root exudation can also quite dramatically alter the pH- and redox-milieu in the rhizosphere. In addition, not only...
Tipo: Journal Article Palavras-chave: EXSUDATION RACINAIRE; RHIZODEPOSITION; DIVERITE MICROBIENNE; MYCORHIZES A ARBUSCULES; ANTIMICROBIEN ROOT EXUDATION; RHIZODEPOSITION; MICROBIAL DIVERSITY; RHIZOSPHERE BACTERIA; MYCORRHIZAL FUNGI; ARBUSCULAR MYCORRHIZA; ECTOMYCORRHIZA; ANTIMICROBIALS; SIGNALLING COMPOUNDS; PLANT GROWTH PROMOTION; BIOLOGICAL CONTROL; "BIASED RHIZOSPHERE CONCEPT".
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011720427a9&uri=/notices/prodinra1/2011/05/
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Repeated sequence RS alpha is diagnostic for Bradyrhizobium japonicum and Bradyrhizobium elkanii Inra
Hartmann, A.; Gomez, M.; Giraud, J.J.; Revellin, C..
The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα. Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated.
Tipo: Journal Article Palavras-chave: BRADYRHIZOBIUM JAPONICUM; BRADYRHIZOBIUM ELKANII; NODULATION; FIXATION BIOLOGIQUE DE L'AZOTE; HYBRIDATION D'ADN.
Ano: 1996 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9600032540059716&uri=/notices/prodinra1/2010/12/
Registros recuperados: 7
Primeira ... 1 ... Última
 

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