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	Provedor de dados BJMBR (1) BJM (1) Autor He,Ying (2) Hou,Deng-Yong (1) Huang,Jing (1) Luo,Qun (1) Shen,Xian-Rong (1) Wen,Xiaorong (1) Zhang,Jian-Guo (1) Zhang,Mei (1) Zhou,Chenyun (1) Mais... Palavra-chave Alkane hydroxylase (1) Biodegradation (1) Carotid artery (1) Diabetes mellitus (1) Diabetic foot (1) Diesel oil (1) Escherichia coli DH5α (1) Expression (1) Intima-media thickness (1) Peripheral arterial disease (1) Plaque (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2015 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Carotid intima-media thickness and plaques in internal carotid artery as surrogate markers of lower limb arterial lesions in Chinese patients with diabetic foot BJMBR Zhang,Mei; Wen,Xiaorong; Zhou,Chenyun; Huang,Jing; He,Ying. Peripheral arterial disease (PAD) can impair healing of diabetic foot (DF) in patients with diabetes mellitus. To determine whether carotid intima-media thickness (CIMT) can predict lower limb arterial lesions in patients with DF, this cross-sectional study enrolled patients with DF at West China Hospital (China) between January 2012 and December 2015. Ultrasonography was used to measure CIMT, assess the internal carotid arteries (ICA) for plaques, and evaluate lower limb segmental arteries for stenosis. The optimal CIMT cutoff for detecting lower limb PAD was determined by receiver operating characteristic (ROC) curve analysis. Factors associated with PAD were identified by logistic regression analyses. A total of 167 patients (mean age: 69.7±10.3 years;... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetes mellitus; Diabetic foot; Carotid artery; Intima-media thickness; Plaque; Peripheral arterial disease. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000700611 GPo1 alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortium BJM Luo,Qun; He,Ying; Hou,Deng-Yong; Zhang,Jian-Guo; Shen,Xian-Rong. To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkane hydroxylase; Expression; Escherichia coli DH5α; Biodegradation; Diesel oil. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300649 Registros recuperados: 2 Primeira ... 1 ... Última

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