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Effect of hepatocyte growth factor and angiotensin II on rat cardiomyocyte hypertrophy 56
Chen,Ai-Lan; Ou,Cai-Wen; He,Zhao-Chu; Liu,Qi-Cai; Dong,Qi; Chen,Min-Sheng.
Angiotensin II (Ang II) plays an important role in cardiomyocyte hypertrophy. The combined effect of hepatocyte growth factor (HGF) and Ang II on cardiomyocytes is unknown. The present study was designed to determine the effect of HGF on cardiomyocyte hypertrophy and to explore the combined effect of HGF and Ang II on cardiomyocyte hypertrophy. Primary cardiomyocytes were isolated from neonatal rat hearts and cultured in vitro. Cells were treated with Ang II (1 µM) alone, HGF (10 ng/mL) alone, and Ang II (1 µM) plus HGF (10 ng/mL) for 24, 48, and 72 h. The amount of [³H]-leucine incorporation was then measured to evaluate protein synthesis. The mRNA levels of β-myosin heavy chain and atrial natriuretic factor were determined by real-time PCR to evaluate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II; Hepatocyte growth factor; Cardiomyocyte; Hypertrophy.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200007
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