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Registros recuperados: 19
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Anesthesia and sedation of map treefrog (Hypsiboas geographicus) tadpoles with essential oils Ciência Rural
Salbego,Joseânia; Maia,Janna Laely dos Santos; Toni,Cândida; Rodrigues,Amanda Sousa Silva; Sousa,Elen Monique Oliveira; Silva,Lenise Vargas Flores da; Mourão,Rosa Helena Veras; Barata,Lauro Euclides Soares; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
ABSTRACT: The goal of this study was to investigate the sedative and anesthetic properties of essential oils (EOs) in map treefrog tadpoles (Hypsiboas geographicus) and to determine the sedation and deep anesthesia induction times as well as the recovery time. The tadpoles were exposed to one of the EOs from three plant species: Aniba rosaeodora (EOAR - 25, 50, 100 or 200µL L-1), Lippia origanoides (EOLO - 13, 25, 50, 100 or 200µL L-1), and Lippia alba (either chemotype citral [EOL-C - 25, 50, 100 or 200µL L-1] or linalool [EOL-L - 50, 75, 100 or 200µL L-1]) (n = 8 per replicate). The tadpoles exposed to 25 and 50µL L-1 EOL-C and EOL-L, respectively, were not anesthetized within 30min (the maximum time of observation), and those exposed to 200µL L-1 EOLO...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amphibians; Animal welfare; Natural anesthetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001100403
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Anesthesia and transport of fat snook Centropomus parallelus with the essential oil of Nectandra megapotamica(Spreng.) Mez Neotropical Ichthyology
Tondolo,Juliana Simoni Moraes; Amaral,Lúcio de Paula; Simões,Larissa Novaes; Garlet,Quelen Iane; Schindler,Bianca; Oliveira,Teodoro Martins; Silva,Bruno Ferreira da; Gomes,Levy de Carvalho; Baldisserotto,Bernardo; Mallmann,Carlos Augusto; Heinzmann,Berta Maria.
This study analyzed the chemical composition and anesthetic potential of essential oil (EO) of Nectandra megapotamica in fat snook (Centropomus parallelus). For the extraction of EO by hydrodistillation, leaves were separated in young (EO-Y) or old (EO-O), and the chemical composition of the EOs was determined by CG-MS. The anesthetic potential was assessed by the evaluation of induction and recovery time of anesthesia and stress response from anesthesia and transport. Three experiments were carried out: i) four different concentrations of each EO were tested to evaluate anesthesia induction and recovery time; ii) two concentrations of EO-O were tested for the evaluation of its effects on stress parameters (glucose, lactate, and Na+ and K+ plasma levels)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Ionoregulation; Sedation; Stress; Terpenoids.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300667
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Anesthetic activity of Brazilian native plants in silver catfish (Rhamdia quelen) Neotropical Ichthyology
Silva,Lenise de Lima; Silva,Daniela Thomas da; Garlet,Quelen Iane; Cunha,Mauro Alves; Mallmann,Carlos Augusto; Baldisserotto,Bernardo; Longhi,Solon Jonas; Pereira,Ana Maria Soares; Heinzmann,Berta Maria.
There is an increasing demand for inexpensive and safe anesthetics that can reduce fish stress caused by some procedures such as capture and handling. In this context, the present study evaluated the potential of essential oils (EO) of three Brazilian native plants (Hesperozygis ringens, Lippia sidoides and Ocotea acutifolia) as anesthetics for the silver catfish - Rhamdia quelen. Moreover, an analysis was made of the chemical composition of these oils and their influence on stress parameter. EO of H. ringens and O. acutifolia were effective as anesthetics, without behavioral side effects. EO of O. acutifolia (150 µL L-1) promoted an increase in blood glucose level. Regarding to the composition, pulegone accounts for 96.63% of the EO of H. ringens, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Hesperozygis ringens; Lippia sidoides; Ocotea acutifolia; Stress.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200443
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Anesthetic activity of the essential oil of Ocimum americanum in Rhamdia quelen (Quoy & Gaimard, 1824) and its effects on stress parameters Neotropical Ichthyology
Silva,Lenise de Lima; Garlet,Quelen Iane; Koakoski,Gessi; Abreu,Murilo Sander de; Mallmann,Carlos Augusto; Baldisserotto,Bernardo; Barcellos,Leonardo José Gil; Heinzmann,Berta Maria.
ABSTRACT The aim of this study was to evaluate the anesthetic activity of the essential oil (EO) of Ocimum americanum L. in silver catfish (Rhamdia quelen ). In the first experiment, the depressor effects and chemical composition of the leaf EO (LEO) and inflorescence EO (IEO) were compared. Juveniles (n = 10) were placed in aquaria containing different concentrations of EO (25 - 500 mg L-1) to determine the point at which anesthesia was induced and the length of the recovery period. In the following experiment, the effects of 300 and 500 mg L-1 LEO exposure on stress parameters (plasma cortisol, glucose and sodium levels) after air exposure for 1 min were assayed. Fish (n = 10 per sampling time) were sampled immediately or transferred to anesthetic-free...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Basil; Cortisol; Glucose; Sodium.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000400715
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Anesthetic induction and recovery of Hippocampus reidi exposed to the essential oil of Lippia alba Neotropical Ichthyology
Cunha,Mauro Alves da; Silva,Bruno Ferreira da; Delunardo,Frederico Augusto Cariello; Benovit,Simone Cristina; Gomes,Levy de Carvalho; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
The aim of this study was to identify the times of anesthetic induction and recovery in slender seahorses (Hippocampus reidi) that were exposed to the essential oil of Lippia alba (EO), as well as the efficacy of EO as a stress-reducing agent in the transport of this species. Slender seahorses were placed in 1-L aquaria containing different concentrations of EO (0, 10, 20, 50, 150, 300 and 450 µL L-1), and after induction, fish were transferred to aquaria that were free of anesthetic to evaluate their recovery time. In an additional experiment, slender seahorses were transported in plastic bags with 15 µL L-1 of EO for 4 or 24 h. The increased concentration of EO proportionally decreased the time required for the induction of anesthesia. EO treatment (15...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood glucose; Leukocyte count; Seahorse; Stress.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252011000300022
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Anesthetic induction and recovery time of Centropomus parallelus exposed to the essential oil of Aloysia triphylla Ciência Rural
Parodi,Thaylise Vey; Santos,Celina Alcoforado dos; Veronez,Alexandra; Gomes,Levy de Carvalho; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
ABSTRACT: This study analyzed the effectiveness of the essential oil of Aloysia triphylla (EOA) as an anesthetic and stress reducing agent in the transport of fat snook ( Centropomus parallelus ). Juveniles were exposed to different concentrations of EOA (seawater-adapted: 25-300μL L-1; freshwater-adapted: 200μL L-1) to identify the anesthetic induction and recovery times. In an additional experiment, seawater-adapted fat snook were transported in plastic bags with 10 or 20μL L-1 EOA for 6 or 24h. The increased concentration of EOA proportionally decreased the time required for anesthesia induction. Mortality was 30 and 70% in fat snook transported with 10 and 20µL L-1, respectively, 24h after transport. The addition of 20µL L-1 EOA significantly reduced...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Blood parameters; Fat snook; Natural product; Transport.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202142
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Anesthetic potential of the essential oils of Lippia alba and Lippia origanoides in Tambaqui juveniles Ciência Rural
Silva,Hugo Napoleão Pereira da; Carvalho,Brenna Celina Ferreira de; Maia,Janna Laely dos Santos; Becker,Alexssandro Geferson; Baldisserotto,Bernardo; Heinzmann,Berta Maria; Mourão,Rosa Helena Veras; Silva,Lenise Vargas Flores da.
ABSTRACT: The aim of this study was to investigate the anesthetic effect of the essential oils (EOs) of Lippia alba (citral and linalool chemotypes, EOLA-C and EOLA-L respectively) and Lippia origanoides (chemotype carvacrol, EOLO-Ca) on tambaqui (Colossoma macropomum) juveniles. The tested concentrations of the L. alba and L. origanoides EOs were: 25, 50, 100, and 200 μL/L. Time required to reach the stages of sedation, deep anesthesia, and recovery, as well as ventilatory frequency, were monitored for each EO concentration. The results indicated that all EO concentrations tested induced tambaqui anesthesia and that the ventilatory frequency was altered by the EOs. L. origanoides showed the shortest time to induce sedation and anesthesia, but had long...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaesthesia; Carvacrol; Citral; Linalool; Ventilatory frequency.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600402
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Chemical composition and antibacterial activity of Aloysia triphylla (L'Hérit) Britton extracts obtained by pressurized CO2 extraction BABT
Parodi,Thaylise Vey; Vargas,Agueda Palmira de Castagna; Krewer,Carina; Flores,Érico Marlon de Moraes; Baldisserotto,Bernardo; Heinzmann,Berta Maria; Oliveira,José Vladimir de; Popiolski,Ariana Secco; Minozzo,Mariane.
This study investigated the chemical composition of five different extracts of Aloysia triphylla and their activity against Aeromonas sp. The extracts were obtained from the dried leaves by pressurized CO2 extraction at 30, 50 and 70ºC, and 100, 150, and 200 bar, and analyzed by GC/FID and GC-MS. The antibacterial activity was assayed by the microdilution method. The tested microorganisms comprised seven Aeromonas isolates obtained from the kidney of infected silver catfish, Rhamdia quelen. The yield, chemical composition and antibacterial activity of the extracts were dependent on the extraction conditions. Mono and sesquiterpenoids were the major constituents of all the extracts and the highest extraction yield was obtained at 70ºC and 200 bar. A....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aloysia triphylla; Pressurized carbon dioxide extraction; Aeromonas sp.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200014
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Citral as food additive for common snook - zootechnical parameters and digestive enzymes Ciência Rural
Michelotti,Bruna Tomazetti; Mori,Natacha Cossettin; Magnotti,Caio Cesar Franca; Heinzmann,Berta Maria; Almeida,Ana Paula Gottlieb; Cerqueira,Vinicius Ronzani; Baldisserotto,Bernardo.
ABSTRACT: Essential oils of plants whose main compound is citral showed beneficial effects when added to fish feed. The objective of the present study was to evaluate the dietary effect of the addition of citral on zootechnical parameters and digestive enzyme activities of Centropomus undecimalis. Juveniles were fed for 45 days with diets containing different amounts of citral (0.0 - control, 0.5, 1.0, and 2.0 mL per kg of diet). The water quality parameters were kept stable during the experiment and no mortality was observed. At the end of the experimental period, the treatment 0.5 mL citral per kg of diet had the lowest weight gain and specific growth rate, and the highest feed conversion, while the same parameters did not differ between the other...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Centropomus undecimalis; Amylase; Pepsin; Lipase; Growth; Marine fish farming.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400651
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Essential oil of Lippia alba in the transport of Nile tilapia Ciência Rural
Hohlenwerger,Janis Cumming; Baldisserotto,Bernardo; Couto,Ricardo David; Heinzmann,Berta Maria; Silva,Daniela Thomas da; Caron,Braulio Otomar; Schmidt,Denise; Copatti,Carlos Eduardo.
ABSTRACT: This study aimed to examine the action of the essential oil of Lippia alba (EOLA) in the stress response for transport of Nile tilapia Oreochromis niloticus . The fish were transported into three treatments (in triplicate): control, 10 and 20 µL L-1 EOLA, with loading density of 15 fish/plastic bags for 8h. Plasma glucose levels were significantly decreased in fish exposed to 20µL L-1 EOLA in comparison with the control group and fish exposed to 10µL L-1 EOLA, but the plasma cortisol, lactate and paraoxonase levels were similar. Un-ionized ammonia and ventilatory rate demonstrated a significant reduction in the treatments with the use of EOLA. In conclusion the use of 20µL L-1 EOLA is indicated for Nile tilapia transport.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oreochromis niloticus; Stress; Glucose; Cortisol; Ventilatory rate.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000300403
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Essential oils from Citrus x aurantium and Citrus x latifolia (Rutaceae) have anesthetic activity and are effective in reducing ion loss in silver catfish ( Rhamdia quelen ) Neotropical Ichthyology
Lopes,Jane Mello; Souza,Carine de Freitas; Schindler,Bianca; Pinheiro,Carlos Garrido; Salbego,Joseânia; Siqueira,Jefferson Costa de; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
ABSTRACT This study investigated the anesthetic effect of the essential oils (EOs) from the peel of Citrus x aurantium (EOCA) and Citrus x latifolia (EOCL) on silver catfish Rhamdia quelen. Fish were exposed to different concentrations of EOCA and EOCL to determine time of anesthesia induction and recovery. Induction of anesthesia was observed in all fish exposed to 400, 600 or 800 μL L−1 EOCA and 300, 400 or 500 μL L−1 EOCL. Another group of fish were exposed for 8 h to 50, 100, or 200 μL L−1 of either EOs. Overall, fish exposed to ethanol and both EOs presented higher ventilatory frequencies (VF) than the control group throughout the 8 h of exposure. Net ion (Na+, K+ and Cl−) effluxes and ammonia excretion were significantly lower in fish exposed to 50,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Ion fluxes; Lemon oil; Orange oil; Ventilatory frequency.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000200204
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Estabilidade lipídica de filés de carpa húngara congelados tratados com extratos de Lippia Alba Ciência Rural
Veeck,Ana Paula de Lima; Klein,Bruna; Ruviaro,Amanda Roggia; Quatrin,Andréia; Ferreira,Lauren Fresinghelli; Daniel,Ana Paula; Piccolo,Jaqueline; Oliveira,Maurício Schneider; Mallmann,Carlos Augusto; Heinzmann,Berta Maria; Emanuelli,Tatiana.
O objetivo do presente estudo foi avaliar o efeito da aspersão de extratos de Lippia alba na estabilidade lipídica de filés de carpa húngara armazenados a -18±2°C. Filés não tratados (controle sem aspersão) ou aspergidos (1mL 10g-1 de filé) com água destilada (controle água destilada) ou com extratos de L. alba (0,10g mL-1) hidrometanólico ou aquoso foram analisados durante o armazenamento nos dias zero, 90 e 180. Independente do tempo de congelamento, o extrato hidrometanólico reduziu os valores de dienos conjugados (DC) dos filés em relação aos demais tratamentos, além de reduzir os valores de ácidos graxos livres aos 90 dias (P<0,05). O extrato aquoso resultou em maior teor de peróxidos após 180 dias de congelamento comparado aos demais tratamentos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lippia Alba; Pescado; Cyprinus carpio; Antioxidante.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601113
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Oxidative stability during frozen storage of fillets from silver catfish (Rhamdia quelen ) sedated with the essential oil of Aloysia triphylla during transport Ciência Rural
Daniel,Ana Paula; Ferreira,Lauren Fresinghelli; Klein,Bruna; Ruviaro,Amanda Roggia; Quatrin,Andréia; Parodi,Thaylise Vey; Zeppenfeld,Carla Cristina; Heinzmann,Berta Maria; Baldisserotto,Bernardo; Emanuelli,Tatiana.
ABSTRACT: This research aimed to evaluate whether the essential oil of Aloysia triphylla (EOAT) used in vivo as a sedative in the water for transporting fish could increase the oxidative stability of silver catfish (Rhamdia quelen) fillets during frozen storage. The chemical composition of EOAT and of fillets from fish exposed to EOAT (0, 30 or 40µL L-1) were assessed. The pH and lipid oxidation parameters (conjugated dienes, CD; thiobarbituric acid-reactive-substances, TBARS) were evaluated in the fillets throughout the storage period (-18±2oC/17 months). The main compounds found in EOAT were α- and β-citral. Treatment with EOAT did not modify the proximate composition of the fillets, but 40µL L-1 EOAT reduced pH levels when compared to the control...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipid oxidation; Transport; Fish; TBARS; Natural anesthetic.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300560
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Physiological responses of Rhamdia quelen (Siluriformes: Heptapteridae) to anesthesia with essential oils from two different chemotypes of Lippia alba Neotropical Ichthyology
Souza,Carine de Freitas; Baldissera,Matheus Dellaméa; Salbego,Joseânia; Lopes,Jane Mello; Vaucher,Rodrigo de Almeida; Mourão,Rosa Helena Veras; Caron,Braulio O.; Heinzmann,Berta Maria; Silva,Lenise Vargas Flôres da; Baldisserotto,Bernardo.
ABSTRACT The aim of this study was to evaluate if Lippia alba has different chemotypes according to the chemical composition of the essential oil (EO) considering collection site, and if the EO may have different effects on blood and plasma parameters in silver catfish, Rhamdia quelen, during and immediately after anesthesia. The citral (EO-C) and linalool (EO-L) chemotypes were identified, and both presented similar anesthetic effects for silver catfish. Fish were exposed to two concentrations of each EO, which induced slow and fast anesthesia (100 and 300 µL L-1, respectively). Blood ions did not change at any time of anesthesia induction and recovery and, therefore, the electrolyte balance was not altered. Blood gases oscillated through all exposure and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood gas; Cortisol; Glucose; Plasma ions; Silver Catfish.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252017000100203
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Piper gaudichaudianum Kunth: Seasonal Characterization of the Essential Oil Chemical Composition of Leaves and Reproductive Organs BABT
Schindler,Bianca; Heinzmann,Berta Maria.
ABSTRACT This study describes a comparative analysis of the essential oil (EO) chemical composition of leaves and reproductive organs (inflorescences and fruits) of Piper gaudichaudianum during the seasons of a year in order to determine the best collection time and the most suitable plant organ to obtain this extractive. The chemical composition of EO obtained from fresh leaves was compared to the dried ones, to verify if the drying process interferes in the extractive quality. The leaves were collected from a native population of Santa Maria, RS, Brazil, twice in each season, in triplicate, while inflorescences and fruits were sampled when they were present. The EO was obtained by hydrodistillation of the different plant organs for 3 h. The 20 EO samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pariparoba; Plant extraction; Medicinal plant; Post-harvest processing.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100603
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Relaxing effect of eugenol and essential oils in Pomacea canaliculata Ciência Rural
Bianchini,Adriane Erbice; Cunha,Jessyka Arruda da; Brusque,Isabel Cristina Markowski; Pinheiro,Carlos Garrido; Schindler,Bianca; Heinzmann,Berta Maria; Baldisserotto,Bernardo.
ABSTRACT: This study evaluated the potential relaxing and/or molluscicidal effects of eugenol and essential oils of Origanum majorana, Ocimum americanum, Hesperozygis ringens, and Piper gaudichaudianum in the gastropod Pomacea canaliculata. Compounds were tested at concentrations of 100, 250, 500, and 750µL L-1 to evaluate the relaxing effects. In the second experiment, animals were exposed to 10, 25, and 50µL L-1 of essential oils of H. ringens and P. gaudichaudianum for a period of 24h for the evaluation of molluscicidal effects. Eugenol and essential oils of O. majorana and O. americanum showed relaxing effects at ≥250µL L-1, but the essential oils of H. ringens and P. gaudichaudianum did not promote relaxing or molluscicidal effects within the times...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anesthesia; Golden apple snail; Mollusk; Ocimum americanum; Origanum majorana.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000403
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Seasonal influence on the essential oil production of Nectandra megapotamica (Spreng.) Mez BABT
Amaral,Lúcio de Paula; Tondolo,Juliana Simoni Moraes; Schindler,Bianca; Silva,Daniela Thomas da; Pinheiro,Carlos Garrido; Longhi,Solon Jonas; Mallmann,Carlos Augusto; Heinzmann,Berta Maria.
This study evaluated the seasonal influence on the yield and chemical composition of the essential oil (EO) of Nectandra megapotamica. Fresh young (YL) and old leaves (OL) obtained from three trees in each season (Nov/2010 to Sep/2011) collected in Santa Maria-RS were hydrodistilled in triplicate. The chemical composition was determined by the gas chromatography coupled to mass spectrometry (GC-MS) and the yield on dry basis was evaluated by two-way ANOVA (seasons, development stage). Spring (Sp) and summer (Su) showed higher average incomes (0.45 and 0.33%), which occurred when flowering, fruiting, and growth of YL and senescence of OL took place, while autumn (Au) presented the lowest yield (0.25%) during the rustification of OL. The highest yield was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lauraceae; Phenology; Variability of volatile oils; Terpenes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100012
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Stress-reducing and anesthetic effects of the essential oils of Aloysia triphylla and Lippia alba on Serrasalmus eigenmanni (Characiformes: Serrasalmidae) Neotropical Ichthyology
Almeida,Ana Paula Gottlieb; Correia,Tiago Gabriel; Heinzmann,Berta Maria; Val,Adalberto Luis; Baldisserotto,Bernardo.
ABSTRACT Anesthetic effect of Aloysia triphylla and Lippia alba essential oils (EOs) in the Amazonian fish Serrasalmus eigenmanni was evaluated. The fish were placed in aquaria containing A. triphylla or L. alba EOs (25 to 200 µL L-1). Then, fish were transferred to aquaria containing EO-free water to evaluate their recovery time. In another experiment, fish were transferred to aquaria containing A. triphylla or L. alba EOs (3 to 10 µL L-1) and swimming behavior was analyzed for up to 240 min of exposure. Water samples were collected at 0 and 240 min and blood samples were collected at 240 min. Tested concentrations induced all stages of anesthesia, except 25 µL L-1 A. triphylla EO and 50 µL L-1 L. alba EO, which only induced sedation. Prolonged exposure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazonian blackwaters; Anesthesia; Ion fluxes; Piranha; Stress.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000200217
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Use of Frozen Leaves for Morpho-anatomical Characterization of Nectandra megapotamica (Spreng.) Mez., Lauraceae BABT
Costa,Leonardo Severo da; Reiniger,Lia Rejane Silveira; Trindade,Helena; Heinzmann,Berta Maria; Bianchini,Nadia Helena.
Abstract Nectandra megapotamica (Spreng.) Mez. it is a native tree species of the Atlantic Forest, commonly known as canela-preta. The species has some anti-inflammatory, antitumor and antirheumatic properties among others. In this work the use of frozen plant material for microscopy analysis was tested. In addition, the leaf morpho-anatomy of the species was characterized which allowed to perform a structural description and to identify structures of secretion and storage of essential oil. The plant material was prepared for analyzes in optical, fluorescence and scanning electron microscopy. The leaf anatomy shows glabrous epidermis, unistratified, paracytic stomata, absence of trichomes, polyhedral epidermal cells. Some typical family characteristics...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Forest species; New methodology; Leaf anatomy; Essential oil; Secretory cavities.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100423
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