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Anti-Biofilm Effect of Biodegradable Coatings Based on Hemibastadin Derivative in Marine Environment 5
Le Norcy, Tiffany; Niemann, Hendrik; Proksch, Peter; Linossier, Isabelle; Vallee-rehel, Karine; Hellio, Claire; Fay, Fabienne.
Dibromohemibastadin-1 (DBHB) is an already known potent inhibitor of blue mussel phenoloxidase (which is a key enzyme involved in bioadhesion). Within this study, the potentiality of DBHB against microfouling has been investigated. The activity of DBHB was evaluated on key strains of bacteria and microalgae involved in marine biofilm formation and bioassays assessing impact on growth, adhesion and biofilm formation were used. To assess the efficiency of DBHB when included in a matrix, DBHB varnish was prepared and the anti-microfouling activity of coatings was assessed. Both in vitro and in situ immersions were carried out. Confocal Laser Scanning Microscopy (CLSM) was principally used to determine the biovolume and average thickness of biofilms developed...
Tipo: Text Palavras-chave: Antifouling; Biodegradable coating; Hemibastadin; Sponges; Marine bacteria; Microalgae.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00616/72812/72626.pdf
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Antihypoxic, nephroprotective and antioxidant properties of hydro-alcoholic extract of loquat flowers 5
Nabavi, Seyed Fazel; Nabavi, Seyed Mohammad; Moghaddam, Akbar Hajizadeh; Hellio, Claire; Ebrahimzadeh, Mohammad Ali.
Objectives: Loquat is well known Chinese medicinal herb with numerous traditional usages. The fact of the promising effect of loquat on different diseases is approved by a plethora of recent studies. Methods: In the present study antihypoxic, antioxidant and nephroprotective activity of hydro-alcoholic extract of Eriobotrya japonica flower was an investigated employing different in vivo and in vitro assay system. The chemical analysis of Eriobotrya japonica flowers, using atomic absorption spectroscopy, highlighted the presence of eight elements. Gallic acid, rutin and quercetin were determined by HPLC/DAD. Results: Results indicated that the hydro-alcoholic extract has a protective effect against hypoxia induced lethality in mice. Extract at doses of 200...
Tipo: Text Palavras-chave: Free radical; Eriobotrya japonica; Gentamicin; Rutin.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00299/40980/73190.pdf
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Bioactive Bromotyrosine Derivatives from the Pacific Marine Sponge Suberea clavata (Pulitzer-Finali, 1982) 5
Moriou, Céline; Lacroix, Damien; Petek, Sylvain; El-demerdash, Amr; Trepos, Rozenn; Leu, Tinihauarii Mareva; Florean, Cristina; Diederich, Marc; Hellio, Claire; Debitus, Cecile; Al-mourabit, Ali.
Chemical investigation of the South-Pacific marine sponge Suberea clavata led to the isolation of eight new bromotyrosine metabolites named subereins 1–8 (2–9) along with twelve known co-isolated congeners. The detailed configuration determination of the first representative major compound of this family 11-epi-fistularin-3 (11R,17S) (1) is described. Their chemical characterization was achieved by HRMS and integrated 1D and 2D NMR (nuclear magnetic resonance) spectroscopic studies and extensive comparison with literature data. For the first time, a complete assignment of the absolute configurations for stereogenic centers C-11/17 of the known members (11R,17S) 11-epi-fistularin-3 (1) and 17-deoxyfistularin-3 (10) was determined by a combination of...
Tipo: Text Palavras-chave: Sponge; Verongiida; Suberea clavata; Bromotyrosine; Fistularin-3; Acetylcholinesterase inhibition; Antifouling.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00684/79581/82258.pdf
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Biomimetic Approaches for the Development of New Antifouling Solutions: Study of Incorporation of Macroalgae and Sponge Extracts for the Development of New Environmentally-Friendly Coatings 5
Sánchez-lozano, Ilse; Hernández-guerrero, Claudia Judith; Muñoz-ochoa, Mauricio; Hellio, Claire.
Biofouling causes major economic losses in the maritime industry. In our site study, the Bay of La Paz (Gulf of California), biofouling on immersed structures is a major problem and is treated mostly with copper-based antifouling paints. Due to the known environmental effect of such treatments, the search for environmentally friendly alternatives in this zone of high biodiversity is a priority to ensure the conservation and protection of species. The aim of this work was to link chemical ecology to marine biotechnology: indeed, the natural defense of macroalgae and sponge was evaluated against biofoulers (biofilm and macrofoulers) from the same geographical zone, and some coatings formulation was done for field assays. Our approach combines in vitro and...
Tipo: Text Palavras-chave: Adhesion; Algae; Antifouling; Biofilm; Extracts; Field assays; Sponges; Haliclona caerulea; Laurencia gardneri; Sargassum horridum; Ircinia sp.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00589/70074/68047.pdf
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Demonstration of a true phenoloxidase activity and activation of a ProPO cascade in Pacific oyster, Crassostrea gigas (Thunberg) in vitro 5
Hellio, Claire; Bado Nilles, Anne; Gagnaire, Beatrice; Renault, Tristan; Thomas-guyon, Helene.
The prophenoloxidase (ProPO) system is the origin of melanin production and is considered to be an innate defence mechanism in invertebrates. In different bivalve species, phenoloxidase (PO) is present in the haemolymph as an inactive form of ProPO. The present study focuses on the Pacific adult oyster, Crassostrea gigas, an economically important bivalve species along French coasts. The results indicate that many factors may inhibit the PO-like activity. These include: phenylthiourea (PTU), sodium diethylthiocarbamate (DETC), beta-mercaptoethanol and tropolone, which repressed the spontaneous PO activity. The activation of PO-like activity in C. gigas acellular fraction by lipopolysaccharide (LPS) involved participation of other factors, including at...
Tipo: Text Palavras-chave: Prophenoloxidase; Phenoloxidase; Oyster; Immunity; Defence; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2926.pdf
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Exploring Antifouling Activity of Biosurfactants Producing Marine Bacteria Isolated from Gulf of California 5
Alemán-vega, Monserrat; Sánchez-lozano, Ilse; Hernández-guerrero, Claudia J.; Hellio, Claire; Quintana, Erika T..
Biofouling causes major problems and economic losses to marine and shipping industries. In the search for new antifouling agents, marine bacteria with biosurfactants production capability can be an excellent option, due to the amphipathic surface-active characteristic that confers antimicrobial and antibiofilm activities. The aim of this study was to evaluate the antifouling activity of biosurfactants producing marine bacteria from the Gulf of California. The cell free culture supernatant (CFCS) of Bacillus niabensis (S-69), Ralstonia sp. (S-74) (isolated from marine sediment) and of B. niabensis (My-30) (bacteria associated to the sponge Mycale ramulosa) were screened for production of biosurfactants (using hemolysis and drop collapse test, oil...
Tipo: Text Palavras-chave: Adhesion; Biofilm; Biosurfactants; Environmentally friendly antifouling; Field assays; Bacillus niabensis; Ralstoniasp.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00646/75804/76733.pdf
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From Crassostrea gigas oyster larvae adhesion studies to potential biotechnological development of marine adhesives 5
Foulon, Valentin; Boudry, Pierre; Guerard, Fabienne; Hellio, Claire.
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00373/48449/48717.pdf
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From Ecology to Biotechnology, Study of the Defense Strategies of Algae and Halophytes (from Trapani Saltworks, NW Sicily) with a Focus on Antioxidants and Antimicrobial Properties 5
Messina, Concetta Maria; Renda, Giuseppe; Laudicella, Vincenzo Alessandro; Trepos, Rozenn; Fauchon, Marilyne; Hellio, Claire; Santulli, Andrea.
This study aimed at the characterization of the antioxidant power of polyphenol extracts (PE) obtained from the algae Cystoseira foeniculacea (CYS) (Phaeophyta) and from the halophyte Halocnemum strobilaceum (HAL), growing in the solar saltworks of western Sicily (Italy), and at the evaluation of their anti-microfouling properties, in order to correlate these activities to defense strategies in extreme environmental conditions. The antioxidant properties were assessed in the PE based on the total antioxidant activity test and the reducing power test; the anti-microfouling properties of the two PE were evaluated by measuring the growth inhibition of marine fish and shellfish pathogen bacteria as well as marine surface fouling bacteria and microalgae exposed...
Tipo: Text Palavras-chave: Polyphenols; Defenses; Anti-oxidants; Antifouling; Anti-microbial; Cystoseira foeniculacea; Halocnemum strobilaceum.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00605/71675/70090.pdf
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Glycoglycerolipids From Sargassum vulgare as Potential Antifouling Agents 5
Plouguerné, Erwan; De Souza, Lauro M.; Sassaki, Guilherme L.; Hellio, Claire; Trepos, Rozenn; Da Gama, Bernardo A. P.; Pereira, Renato Crespo; Barreto-bergter, Eliana.
Unraveling new environmentally friendly antifouling (AF) agents is one of the major quests currently facing marine biotechnology. Marine macroalgae represent a rich source of new compounds with promising biological properties, including AF activity, but most of the macroalgal compounds studied to date are terpenoids or polyphenolics. The aim of this work was to investigate the possible AF role played by a usually neglected class of marine natural products: glycolipids (monogalactosyldiacylglycerols – MGDG, digalactosyldiacylglycerols – DGDG and sulfoquinovosyldiacylglycerols – SQDG) isolated from the Phaeophyceae Sargassum vulgare collected along the coast of south-eastern Brazil. Among the 18 extracts, fractions and sub-fractions tested, 3 sub-fractions...
Tipo: Text Palavras-chave: Sargassum vulgare; Glycoglycerolipids; Antifouling; Microfouling; Macroalgae.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00618/73053/72168.pdf
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In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement 5
Foulon, Valentin; Boudry, Pierre; Artigaud, Sebastien; Guérard, Fabienne; Hellio, Claire.
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the...
Tipo: Text Palavras-chave: Crassostrea gigas; Pacific oyster; Pediveliger larvae; Bioadhesive; Transcriptome.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00475/58677/61172.pdf
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Marine bacteria from the Gulf of California with antimicrofouling activity against colonizing bacteria and microalgae 5
Sanchez-rodriguez, Diana Elizabeth; Ortiz-aguirre, Ismael; Aguila-ramirez, Ruth Noemi; Guadalupe Rico-virgen, Erika; Gonzalez-acosta, Barbara; Hellio, Claire.
One way of reducing the input of pollutants into the marine environment is to enforce the use of non-toxic antifouling paints in marine protected areas. Thus, the purpose of this study was to detect marine microorganisms that secrete inhibitory substances against bacteria and microalgae to avoid biofouling on manmade structures in La Paz bay, B.C.S., Mexico. The inhibitory potential of 125 bacteria was evaluated against biofilm-forming bacteria. Crude extracts were obtained with methanol and ethyl acetate from 16 bacterial strains that exhibited antagonistic and antibacterial activity in a preliminary screening. Antibacterial and antimicroalgal assays were performed using crude extracts, the minimum inhibitory concentration (MIC) was determined. The...
Tipo: Text Palavras-chave: Epibionts; Extracts; Shewanella; Staphylococcus; Biofouling; Antifouling.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00616/72762/72271.pdf
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New Antimalarial and Antimicrobial Tryptamine Derivatives from the Marine Sponge Fascaplysinopsis reticulata 5
Campos, Pierre-eric; Pichon, Emmanuel; Moriou, Céline; Clerc, Patricia; Trépos, Rozenn; Frederich, Michel; De Voogd, Nicole; Hellio, Claire; Gauvin-bialecki, Anne; Al-mourabit, Ali.
Chemical study of the CH2Cl2-MeOH (1:1) extract of the sponge Fascaplysinopsis reticulata collected in Mayotte highlighted three new tryptophan derived alkaloids, 6,6′-bis-(debromo)-gelliusine F (1), 6-bromo-8,1′-dihydro-isoplysin A (2) and 5,6-dibromo-8,1′-dihydro-isoplysin A (3), along with the synthetically known 8-oxo-tryptamine (4) and the three known molecules from the same family, tryptamine (5), (E)-6-bromo-2′-demethyl-3′-N-methylaplysinopsin (6) and (Z)-6-bromo-2′-demethyl-3′-N-methylaplysinopsin (7). Their structures were elucidated by 1D and 2D NMR spectra and HRESIMS data. All compounds were evaluated for their antimicrobial and their antiplasmodial activities. Regarding antimicrobial activities, the best compounds are (2) and (3), with minimum...
Tipo: Text Palavras-chave: Fascaplysinopsis reticulata; Marine sponge; Tryptamine alkaloids; Antimalarial activity; Antimicrobial activity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00485/59664/62722.pdf
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Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae 5
Foulon, Valentin; Artigaud, Sebastien; Buscaglia, Manon; Bernay, Benoit; Fabioux, Caroline; Petton, Bruno; Elies, Philippe; Boukerma, Kada; Hellio, Claire; Guerard, Fabienne; Boudry, Pierre.
Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the...
Tipo: Text
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00462/57379/59452.pdf
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Quorum Sensing Inhibitory and Antifouling Activities of New Bromotyrosine Metabolites from the Polynesian Sponge Pseudoceratina n. sp. 5
Tintillier, Florent; Moriou, Céline; Petek, Sylvain; Fauchon, Marilyne; Hellio, Claire; Saulnier, Denis; Ekins, Merrick; Hooper, John N. A.; Al-mourabit, Ali; Debitus, Cecile.
Four new brominated tyrosine metabolites, aplyzanzines C–F (1–4), were isolated from the French Polynesian sponge Pseudoceratina n. sp., along with the two known 2-aminoimidazolic derivatives, purealidin A (5) and 6, previously isolated, respectively, from the sponges Psammaplysilla purpurea and Verongula sp. Their structures were assigned based on the interpretation of their NMR and HRMS data. The compounds exhibited quorum sensing inhibition (QSi) and antifouling activities against several strains of bacteria and microalgae. To our knowledge, the QSi activity of this type of bromotyrosine metabolite is described here for the first time.
Tipo: Text Palavras-chave: Sponge; Pseudoceratina; Quorum sensing inhibitory activity; Antifouling; Verongiida; Bromotyrosine.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00630/74200/73825.pdf
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SAR of Sponge-Inspired Hemibastadin Congeners Inhibiting Blue Mussel PhenolOxidase 5
Niemann, Hendrik; Hagenow, Jens; Chung, Mi-young; Hellio, Claire; Weber, Horst; Proksch, Peter.
Hemibastadin derivatives, including the synthetically-derived 5,5-dibromohemibastadin-1 (DBHB), are potent inhibitors of blue mussel phenoloxidase (PO), which is a key enzyme involved in the firm attachment of this invertebrate to substrates and, thus, a promising molecular target for anti-fouling research. For a systematic investigation of the enzyme inhibitory activity of hemibastadin derivatives, we have synthesized nine new congeners, which feature structural variations of the DBHB core structure. These structural modifications include, e.g., different halogen substituents present at the aromatic rings, different amine moieties linked to the (E)-2-(hydroxyimino)-3-(4-hydroxyphenyl)propionic acid, the presence of free vs. substituted aromatic hydroxyl...
Tipo: Text Palavras-chave: Antifouling; Hemibastadin; Phenoloxidase; Sponges; Copper; Mytilus edulis.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00273/38432/73167.pdf
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Screening of marine algal extracts for anti-settlement activities against microalgae and macroalgae 5
Hellio, Claire; Berge, Jean-pascal; Beaupoil, Claude; Le Gal, Yves; Bourgougnon, Nathalie.
The ban on the use of TBT-based antifouling paints for boats under 25 m in length has lead to a search for new non-toxic antifoulants. One of the most promising alternative technologies to heavy metal based antifouling paint is the development of antifouling coatings whose active ingredients are naturally occurring compounds from marine organisms. This is based on the principle that marine organisms are also facing the problem of the presence of epibionts on their own surface. In this study, the antifouling activity of a series of aqueous, ethanolic and dichloromethane extracts from 30 algae from the North East Atlantic coast was investigated. The extracts were tested in laboratory assays against species representatives of two major groups of fouling...
Tipo: Text Palavras-chave: Antifouling; Antialgal; Microalgae; Macroalgae; Screening; Settlement; Spores.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/00057/16795/14304.pdf
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Species-Specific Antioxidant Power and Bioactive Properties of the Extracts Obtained from Wild Mediterranean Calendula Spp. (Asteraceae) 5
Messina, Concetta Maria; Troia, Angelo; Arena, Rosaria; Manuguerra, Simona; Ioannou, Theodora; Curcuraci, Eleonora; Renda, Giuseppe; Hellio, Claire; Santulli, Andrea.
n this study we focused on four taxa of the genus Calendula (C. maritima, C. suffruticosa subsp. fulgida, C. arvensis, and the hybrid between the first two ones), collected in Mediterranean area (Sicily). Six extracts for each species were obtained using solvents with increasing polarity (hexane, ethanol 80%, acetone 70%, and water) and through extraction by supercritical fluids (SFE). It has been observed that the solvent with the highest extraction efficiency was ethanol 80% for all species. However, SFE extracts showed high antioxidant activity comparable to the ethanol 80% extract (polyphenol, DPPH, and reducing power method). These findings were confirmed by in vitro analysis (MTT assay) where it was observed that the tested concentration (24 µg/mL),...
Tipo: Text Palavras-chave: Calendula species; Marigold; Supercritical fluid extraction; Bioactivity; Antioxidants; Polyphenols.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00591/70336/68389.pdf
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Sponge-Inspired Dibromohemibastadin Prevents and Disrupts Bacterial Biofilms without Toxicity 5
Le Norcy, Tiffany; Niemann, Hendrik; Proksch, Peter; Tait, Karen; Linossier, Isabelle; Rehel, Karine; Hellio, Claire; Fay, Fabienne.
Since the banning of several families of compounds in antifouling (AF) coatings, the search for environmentally friendly AF compounds has intensified. Natural sources of AF compounds have been identified in marine organisms and can be used to create analogues in laboratory. In a previous study, we identified that dibromohemibastadin-1 (DBHB) is a promising AF molecule, leading to the inhibition of the activity of phenoloxidase, an enzyme involved in the attachment of mussels to surfaces. This paper describes the activity of the DBHB on biofilm formation and its detachment and on bacterial adhesion and communication: quorum sensing. DBHB has an anti-biofilm activity without affecting adhesion of marine and terrestrial bacteria at a dose of 10 mu M....
Tipo: Text Palavras-chave: Biofouling; Biofilm; Bastadin derivative; Microfouling; Quorum sensing; Sponge.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00616/72813/72581.pdf
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The oceans are changing: impact of ocean warming and acidification on biofouling communities 5
Dobretsov, Sergey; Coutinho, Ricardo; Rittschof, Daniel; Salta, Maria; Ragazzola, Federica; Hellio, Claire.
Climate change (CC) is driving modification of the chemical and physical properties of estuaries and oceans with profound consequences for species and ecosystems. Numerous studies investigate CC effects from species to ecosystem levels, but little is known of the impacts on biofilm communities and on bioactive molecules such as cues, adhesives and enzymes. CC is induced by anthropogenic activity increasing greenhouse emissions leading to rises in air and water temperatures, ocean acidification, sea level rise and changes in ocean gyres and rainfall patterns. These environmental changes are resulting in alterations within marine communities and changes in species ranges and composition. This review provides insights and synthesis of knowledge about the...
Tipo: Text Palavras-chave: Biofilm; Biofouling; Bioactive compounds; Climate change.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61686/65641.pdf
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The Recruitment of the Endangered Fan Mussel Pinna nobilis (Linnaeus, 1758) on the Ropes of a Mediterranean Mussel Long Line Farm 5
Theodorou, John A.; James, Ross; Tzovenis, Ioannis; Hellio, Claire.
The fan mussel Pinna nobilis (Linnaeus, 1758) is an endangered bivalve species and is endemic to the Mediterranean Sea. Juvenile animals have been found growing on mussels Mytilus galloprovincialis (Lamarck, 1819) long line aquaculture bounces and ropes within the Maliakos Gulf (Aegean Sea). Animals were sampled from the harvested mussel lines. The results show twelve juvenile P. nobilis per ton of harvested M. galloprovincialis.
Tipo: Text Palavras-chave: Fan mussel; Pinna nobilis; Recruitment; Biofouling; Mediterranean mussel aquaculture; Mytilus galloprovincialis; Maliakos Gulf; Aegean Sea.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00617/72867/71940.pdf
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