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Registros recuperados: 10 | |
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Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie. |
The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days.... |
Tipo: Text |
Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf |
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Harney, Ewan; Dubief, Bruno; Boudry, Pierre; Basuyaux, Olivier; Schilhabel, Markus B.; Huchette, Sylvain; Paillard, Christine; Nunes, Flavia. |
The European abalone Haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. Aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic Vibrio infection. Transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. To generate an extensive transcriptome, we performed next-generation sequencing of RNA on larvae exposed to temperature and pH variation and on haemolymph of adults from two wild populations after experimental infection with Vibrio harveyi. We obtained more than 1.5... |
Tipo: Text |
Palavras-chave: RNA-Seq; Mollusca; Gastropoda; Development; Vibrio harveyi; Climate change. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00318/42940/45103.pdf |
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Roussel, Sabine; Bish, Thomas; Day, Rob; Boudry, Pierre; Huchette, Sylvain; Lambert, Christophe; Lachambre, Sébastien. |
The domestication of Haliotis tuberculata began recently. During this domestication process, abalone may acquire behavioural and physiological traits to become more adapted to their captive environment. These modifications could be the result of intentional selection on production traits, or of unintentional selection due to specific conditions experienced in the farm environment. In order to study this process, progenies of 3 different broodstock origins were studied. Wild abalone, farmed abalone selected for faster growth at 5% selection pressure and randomly selected farmed abalone were used as broodstock. Farmed abalone were the third generation of abalone resulting from systematic mating between wild and farmed broodstock (either males or females were... |
Tipo: Text |
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Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51546/52138.pdf |
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Roussel, Sabine; Poitevin, Pierre; Day, Robert; Le Grand, Fabienne; Stiger‐pouvreau, Valérie; Leblanc, Catherine; Huchette, Sylvain. |
While population foraging behaviour of herbivores has been extensively studied, individual choice is still poorly understood. Very few studies have focused on the individual consistency of foraging behaviour in marine herbivores. Because marine ectotherms are strongly influenced by their environment and because a mixed diet is appropriate for herbivores, we hypothesized that Haliotis tuberculata, a large marine gastropod, would not exhibit significant individual consistency in foraging activity and would display generalist food choices. To test these hypotheses, the behaviour of 120 abalone was studied using a choice test of eight macroalgal species over 3 weeks, with video recording 24 hr a day. In addition, primary components, secondary metabolites and... |
Tipo: Text |
Palavras-chave: Food choice; Foraging activity; Individual consistency; Intraclass correlation; Marine herbivore; Mollusc; Proportion similarity index. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00613/72465/71613.pdf |
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Roussel, Sabine; Caralp, Claire; Leblanc, Catherine; Le Grand, Fabienne; Stiger-pouvreau, Valerie; Coulombet, Céline; Le Goïc, Nelly; Huchette, Sylvain. |
The commercial culture of Haliotis tuberculata has recently started in Europe. As abalone is herbivorous, the use of local collected algae as feed may appear advantageous. The nutritional value of eight monospecific seaweed diets was studied using Palmaria palmata (Rhodophyta), filamentous algae, mainly Gracilaria sp. (Rhodophyta), Enteromorpha sp. and Ulva lactuca (Chlorophyta), together with Saccharina latissima, Saccorhiza polyschides, Laminaria digitata and Laminaria hyperborea (Ochrophyta, Phaeophyceae) and a mixed macroalgal diet. An integrative approach consisted in monitoring the seasonal composition changes of these algae in terms of protein, lipid, soluble carbohydrate, fatty acid and amino-acid contents, and to relate it to seasonal growth and... |
Tipo: Text |
Palavras-chave: Abalone; Algae; Reproduction; Growth; Protein. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00509/62066/66246.pdf |
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Auzoux-bordenave, Stéphanie; Wessel, Nathalie; Badou, Aïcha; Martin, Sophie; M’zoudi, Saloua; Avignon, Solène; Roussel, Sabine; Huchette, Sylvain; Dubois, Philippe. |
Ocean acidification (OA) is a major global driver that leads to substantial changes in seawater carbonate chemistry, with potentially serious consequences for calcifying organisms. Marine shelled molluscs are ecologically and economically important species, providing essential ecosystem services and food sources for other species. Due to their physiological characteristics and their use of calcium carbonate (CaCO3) to build their shells, molluscs are among the most vulnerable invertebrates with regard to OA, with early developmental stages being particularly sensitive to pH changes. This study investigated the effects of CO2-induced OA on juveniles of the European abalone Haliotis tuberculata, a commercially important gastropod species. Six-month-old... |
Tipo: Text |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00599/71135/73504.pdf |
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Lachambre, Sebastien; Huchette, Sylvain; Day, Rob; Boudry, Pierre; Rio-cabello, Antoine; Fustec, Timothee; Roussel, Sabine. |
Abalone growth rate is often identified among important traits to improve through selective breeding. However, the rapid success of some selective breeding plans has sometimes led to negative effects in some aquaculture species due to trade-offs. One of them is the loss of homeostasis of selected animals which results in the inability to resist the stress experienced during the rearing process. In this context, this study aimed to analyze the phenotypic relationships between growth, and physiological and behavioural traits in Haliotis tuberculata under stressful conditions. Eleven traits related to growth, immunity, reproduction and behaviour were recorded under laboratory conditions. A total of 120 adults from wild or farm origin were first monitored... |
Tipo: Text |
Palavras-chave: Haliotis tuberculata; Growth; Behaviour; Physiology; Multivariate analysis; Phenotyping. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00333/44434/44101.pdf |
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Gobet, Angelique; Mest, Laetitia; Perennou, Morgan; Dittami, Simon M.; Caralp, Claire; Coulombet, Celine; Huchette, Sylvain; Roussel, Sabine; Michel, Gurvan; Leblanc, Catherine. |
Background: Holobionts have a digestive microbiota with catabolic abilities allowing the degradation of complex dietary compounds for the host. In terrestrial herbivores, the digestive microbiota is known to degrade complex polysaccharides from land plants while in marine herbivores, the digestive microbiota is poorly characterized. Most of the latter are generalists and consume red, green, and brown macroalgae, three distinct lineages characterized by a specific composition in complex polysaccharides, which represent half of their biomass. Subsequently, each macroalga features a specific epiphytic microbiota, and the digestive microbiota of marine herbivores is expected to vary with a monospecific algal diet. We investigated the effect of four... |
Tipo: Text |
Palavras-chave: Microbe-host interactions; Digestive microbiota; Abalone; Macroalgae; Holobiont. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00489/60071/63368.pdf |
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Lachambre, Sebastien; Day, Rob; Boudry, Pierre; Huchette, Sylvain; Rio-cabello, Antoine; Fustec, Tirnothee; Roussel, Sabine. |
Farming, and thus the domestication of Haliotis tuberculata, began recently. We compared the responses of unselected farmed and wild abalone to stressors that occur on farms. The aim was to determine if the farm environment had induced differences in the behavioural or physiological performances of the abalone. Thirty hatchery-born 3.5 year-old abalone and thirty wild ones were reared under standard farm conditions for 6 months and characterised for 19 traits related to growth, survival, behaviour and immunology. Behavioural and immunological responses to stressors differed between the two stocks. Farmed abalone retracted and swivelled less in reaction to a finger contact. Phagocytosis efficiency was reduced by shaking in abalone from both origins, but the... |
Tipo: Text |
Palavras-chave: Abalone aquaculture; Domestication syndrome; Behavioural adaptation; Responses to stress; Immune response; Farm stressors. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00395/50598/53725.pdf |
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Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre. |
Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by... |
Tipo: Text |
Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf |
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Registros recuperados: 10 | |
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