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Biochemical characterization and cytotoxic effect of the skin secretion from the red-spotted Argentina frog Argenteohyla siemersi (Anura: Hylidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Fusco,Luciano S.; Cajade,Rodrigo; Piñeiro,Jose M.; Torres,Ana M.; Silva,Igor R. F. da; Hyslop,Stephen; Leiva,Laura C.; Pimenta,Daniel C.; Bustillo,Soledad.
Abstract Background: Argenteohyla siemersi (red-spotted Argentina frog) is a casque-headed tree frog species belonging to the Hylidae family. This species has a complex combination of anti-predator defense mechanisms that include a highly lethal skin secretion. However, biochemical composition and biological effects of this secretion have not yet been studied. Methods: The A. siemersi skin secretion samples were analyzed by mass spectrometry and chromatographic analysis (MALDI-TOF/MS, RP-HPLC and GC-MS). Proteins were also studied by SDS-PAGE. Among the biological activities evaluated, several enzymatic activities (hemolytic, phospholipase A2, clotting, proteolytic and amidolytic) were assessed. Furthermore, the cytotoxic activity (cytolysis and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Argenteohyla siemersi; Hylidae; Skin secretion; Tree frog; Cytotoxicity.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100306
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Presynaptic neuromuscular action of a methanolic extract from the venom of Rhinella schneideri toad J. Venom. Anim. Toxins incl. Trop. Dis.
Rostelato-Ferreira,Sandro; Belo,Cháriston A Dal; Leite,Gildo B; Hyslop,Stephen; Rodrigues-Simioni,Léa.
BackgroundRhinella schneideri, previously known as Bufo paracnemis, is a common toad in many regions of Brazil. Its venom exerts important cardiovascular effects on humans and other animals. Although this toad venom has been the subject of intense investigations, little is known about its neuromuscular activity.MethodsThe neurotoxicity of a methanolic extract of R. schneideri venom was tested on mouse phrenic nerve-diaphragm (PND) preparations mounted for conventional twitch tension recording – in response to indirect stimulation – and for electrophysiological measurements.ResultsVenom extract (50 μg/mL) increased the muscle twitch tension in PND preparations but did not significantly alter the resting membrane potential values. Electrophysiological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neurotransmitter release; Ouabain; Presynaptic; Rhinella schneideri; Toad venom.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200332
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Purification and characterization of a hyaluronidase from venom of the spider Vitalius dubius (Araneae, Theraphosidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Sutti,Rafael; Tamascia,Mariana Leite; Hyslop,Stephen; Rocha-e-Silva,Thomaz Augusto Alves.
BackgroundVenom hyaluronidase (Hyase) contributes to the diffusion of venom from the inoculation site. In this work, we purified and characterized Hyase from the venom of Vitalius dubius (Araneae, Theraphosidae), a large theraphosid found in southeastern Brazil. Venom obtained by electrical stimulation of adult male and female V. dubius was initially fractionated by gel filtration on a Superdex® 75 column. Active fractions were pooled and applied to a heparin-sepharose affinity column. The proteins were eluted with a linear NaCl gradient.ResultsActive fractions were pooled and assessed for purity by SDS-PAGE and RP-HPLC. The physicochemical tests included optimum pH, heat stability, presence of isoforms, neutralization by flavonoids and assessment of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hyaluronidase; Venom; Purification; Vitalius dubius.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200312
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