Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 10
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Asynchrony between Antarctic temperature and CO2 associated with obliquity over the past 720,000 years ArchiMer
Uemura, Ryu; Motoyama, Hideaki; Masson-delmotte, Valerie; Jouzel, Jean; Kawamura, Kenji; Goto-azuma, Kumiko; Fujita, Shuji; Kuramoto, Takayuki; Hirabayashi, Motohiro; Miyake, Takayuki; Ohno, Hiroshi; Fujita, Koji; Abe-ouchi, Ayako; Iizuka, Yoshinori; Horikawa, Shinichiro; Igarashi, Makoto; Suzuki, Keisuke; Suzuki, Toshitaka; Fujii, Yoshiyuki.
The delta D temperature proxy in Antarctic ice cores varies in parallel with CO2 through glacial cycles. However, these variables display a puzzling asynchrony. Well-dated records of Southern Ocean temperature will provide crucial information because the Southern Ocean is likely key in regulating CO2 variations. Here, we perform multiple isotopic analyses on an Antarctic ice core and estimate temperature variations at this site and in the oceanic moisture source over the past 720,000 years, which extend the longest records by 300,000 years. Antarctic temperature is affected by large variations in local insolation that are induced by obliquity. At the obliquity periodicity, the Antarctic and ocean temperatures lag annual mean insolation. Further, the...
Tipo: Text
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00496/60788/64966.pdf
Imagem não selecionada

Imprime registro no formato completo
Evaluation of novel oocyst wall protein candidates of Toxoplasma gondii OAK
Salman, Doaa; Okuda, Liria Hiromi; Ueno, Akio; Dautu, George; Zhang, Feifei; Igarashi, Makoto.
Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. Thus, it is considered the key for transmission of T. gondii. Analysis of oocyst wall composition is mandatory therefore; the aim of this study was to identify novel T. gondii oocyst wall proteins and test their use in detection of these oocysts in environmental samples. Five candidates of novel T. gondii oocyst wall proteins (TgOWPs) were identified and named TgOWP8 through TgOWP12. Recombinant protein of TgOWP8 was expressed in E. coli using glutathione S-transferase as fusion protein. Polyclonal antibody was produced and validated by indirect immunofluorescence antibody assay (IFA)....
Palavras-chave: T. gondii oocyst wall protein; Toxoplasma gondii.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4541
Imagem não selecionada

Imprime registro no formato completo
Evaluation of recombinant antigens in combination and single formula for diagnosis of feline toxoplasmosis OAK
Abdelbaset, Abdelbaset Eweda; Alhasan, Hend; Salman, Doaa; Karram, Mohamed Hassan; Rushdi, Mahmoud Abd Ellah; Xuan Xuenan; Igarashi, Makoto.
Cats are the only definitive hosts of Toxoplasma gondii and constitute an essential source of infection to all warm blooded animals and humans. Diagnosis of T gondii infection in cats is fundamental for proper management and control of infection in humans and animals. In the current study, we have evaluated the diagnostic performance of tachyzoite lysate antigen (TLA) and different T. gondii recombinant antigens including surface antigen 2 (SAG2), dense granule proteins 2, 6, 7, 15 (GRA2, GRA6, GRA7, GRA15) and microneme 10 protein (MIC10) in immunoglobulin G enzyme linked-immunosorbent assay (IgG ELISA) using cat serum samples, with reference to latex agglutination test (LAT). Remarkably, TLA showed better performance than other recombinant antigens in...
Palavras-chave: ELISA; Recombinant proteins; Serodiagnosis; Toxoplasmosis.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4574
Imagem não selecionada

Imprime registro no formato completo
Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis OAK
Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan.
Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were...
Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995
Imagem não selecionada

Imprime registro no formato completo
Lack of Sequence Variation of Y Chromosome-Linked Loci in Steller's Sea Lions (Eumetopias jubatus) from Iony Island and the Kuril Islands OAK
Kariya, Tatsuya; Igarashi, Makoto; Wada, Kazuo; Burkanov, Vladimir N.; Koyama, Satoshi; Hoshino, Hiroshi; Oshida, Tatsuo; 五十嵐, 慎; 押田, 龍夫.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4108
Imagem não selecionada

Imprime registro no formato completo
Lactate dehydrogenase in Toxoplasma gondii controls virulence, bradyzoite differentiation, and chronic infection OAK
Abdelbaset, E. Abdelbaset; Fox, Barbara A.; Karram, Mohamed H.; Abd Ellah, Mahmoud R.; Bzik, David J.; Igarashi, Makoto.
In the asexual stages, Toxoplasma gondii stage converts between acute phase rapidly replicating tachyzoites and chronic phase slowly dividing bradyzoites. Correspondingly, T. gondii differentially expresses two distinct genes and isoforms of the lactate dehydrogenase enzyme, expressing LDH1 exclusively in the tachyzoite stage and LDH2 preferentially in the bradyzoite stage. LDH catalyzes the interconversion of pyruvate and lactate in anaerobic growth conditions and is utilized for energy supply, however, the precise role of LDH1 and LDH2 in parasite biology in the asexual stages is still unclear. Here, we investigated the biological role of LDH1 and LDH2 in the asexual stages, and the vaccine strain potential of deletion mutants lacking LDH1, LDH2, or both...
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4451
Imagem não selecionada

Imprime registro no formato completo
Mitochondrial cytochrome b gene sequence diversity among Steller’s sea lion rookeries in the Kuril Islands and the Sea of Okhotsk OAK
Kariya, Tatsuya; Igarashi, Makoto; Wada, Kazuo; Burkanov, Vladimir N.; Koyama, Satoshi; Hoshino, Hiroshi; Oshida, Tatsuo; 五十嵐, 慎; 押田, 龍夫.
http://www.jstage.jst.go.jp/article/mammalstudy/33/3/33_125/_article/-char/ja/
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3038
Imagem não selecionada

Imprime registro no formato completo
Prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower Northern Thailand OAK
Pumidonming, Wilawan; Salman, Doaa; Gronsang, Dulyatad; Abdelbaset, E. Abdelbaset; Sangkaeo, Khamphon; Kawazu, Shin-ichiro; Igarashi, Makoto.
Gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. We investigated the prevalence of gastrointestinal helminths of zoonotic significance in dogs and cats in lower Northern Thailand and utilized molecular tools for species identification of hookworms and Opisthorchis viverrini. Fecal samples of 197 dogs and 180 cats were collected. Overall prevalence of infection using microscopy was 40.1% in dogs and 33.9% in cats. Helminth infection found in both dogs and cats included hookworms, Spirometra spp., Taenia spp., Toxocara spp., 0. viverrini, Strongyloides spp. and Trichuris spp. Hookworms were the most common helminth in dogs, while Spirometra spp. were the most prevalent in cats. Among hookworm infection in dogs and...
Palavras-chave: Cat; Dog; Gastrointestinal helminth; Thailand; Zoonotic parasite.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4452
Imagem não selecionada

Imprime registro no formato completo
The Influence of the regulation of Toxoplasma gondii TgMIC2 transgene on host cell infection OAK
Buates, S.; Xuan, Xuenan; Igarashi, Makoto; Sugimoto, Chihiro; Inoue, Noboru; 玄, 学南; 五十嵐, 慎; 井上, 昇.
Toxoplasma gondii microneme protein 2 (TgMIC2), an apically stored adhesin, was shown to be a key participant involved in the initial attachment and invasion to a host cell. In this study, we had established the clonal line of T. gondii tachyzoites which over-expressed TgMIC2 using the tetracycline repressor (TetR)-based inducible gene expression system. The TgMIC2 over-expression significantly reduced tachyzoite propagation in vitro (p < 0.002) and significantly reduced the parasite virulence in mice (p = 0.002). The over-expression of exogenous TgMIC2 caused the increase of mRNA expression level of endogenous TgMIC2. Wild type parasites mainly expressed TgMIC2115 protein, the microneme store and the surface form of TgMIC2. Over-expression of TgMIC2...
Palavras-chave: Toxoplasma gondii; TgMIC2; Over-expression.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2236
Imagem não selecionada

Imprime registro no formato completo
Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential OAK
Dautu, George; Munyaka, Biscah; Carmen, Gabriella; Zhang, Guohong; Omata, Yoshitaka; Xuan, Xuenan; Igarashi, Makoto.
A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-γ synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific...
Palavras-chave: Toxoplasma gondii; DNA; Deoxyribonucleic acid; DNA immunization; MIC2; Microneme protein-2; M2AP; MIC2 associated protein; AMA1; Apical membrane antigen-1; BAG1; Bradyzoite antigen-1; IgG; Immunoglobulin G.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1031
Registros recuperados: 10
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional