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Registros recuperados: 5
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A molecular survey of Mycoplasma haemocanis in dogs and foxes in Aomori Prefecture, Japan OAK
Sasaki, M.; Ohta, K.; Matsuu, Aya; Hirata, Haruyuki; Ikadai, Hiromi; Oyamada, Takashi.
Palavras-chave: Aomori Prefecture; Dog; Fox; Mycoplasma haemocanis.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2233
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BABESIA CABALLI : ANALYSIS OF CHROMOSOMES SEPARATED BY PULSE FIELD GEL ELECTROPHORESIS OAK
Ikadai, Hiromi; Kudo, Noboru; Oyamada, Takashi; Igarashi, Ikuo; 五十嵐, 郁男.
Palavras-chave: Babesia caballi; Pulsed field gel electrophoresis; Chromosome; Rhoptry gene.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/642
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Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction OAK
Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction(PCR)method.The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001%. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281
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Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis OAK
Jia, Honglin; Zhou, Jinlin; Ikadai, Hiromi; Matsuu, Aya; Suzuki, Hiroshi; Fujisaki, Kozo; Xuan, Xuenan; 鈴木, 宏志; 五十嵐, 郁男; 玄, 学南.
Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These...
Palavras-chave: Babesia gibsoni; Secreted Antigen 1; Identification; Serodiagnosis.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1655
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Purification of Merozoites from In Vitro Cultured Babesia caballi- Infected Equine Erythrocytes OAK
Ikadai, Hiromi; Sato, Masumi; Avarzed, Abgaandorjiin; Nagasawa, Hideyuki; Igarashi, Ikuo; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; 長澤, 秀行; 五十嵐, 郁男.
Palavras-chave: Purification; Merozoite; Babesia caballi.
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/272
Registros recuperados: 5
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