Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 39
Primeira ... 12 ... Última
Imagem não selecionada

Imprime registro no formato completo
Abiotic stress protection by ecologically abundant dimethylsulfoniopropionate and its natural and synthetic derivatives: insights from B acillus subtilis ArchiMer
Broy, Sebastian; Chen, Chiliang; Hoffmann, Tamara; Brock, Nelson L.; Nau-wagner, Gabriele; Jebbar, Mohamed; Smits, Sander H. J.; Dickschat, Jeroen S.; Bremer, Erhard.
Dimethylsulfoniopropionate (DMSP) is an abundant osmolyte and anti-stress compound produced primarily in marine ecosystems. After its release into the environment, microorganisms can exploit DMSP as a source of sulfur and carbon, or accumulate it as an osmoprotectant. However, import systems for this ecophysiologically important compatible solute, and its stress-protective properties for microorganisms that do not produce it are insufficiently understood. Here we address these questions using a well-characterized set of Bacillus subtilis mutants to chemically profile the influence of DMSP import on stress resistance, the osmostress-adaptive proline pool and on osmotically controlled gene expression. We included in this study the naturally occurring...
Tipo: Text
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00242/35302/33910.pdf
Imagem não selecionada

Imprime registro no formato completo
Chemosynthetic ectosymbionts associated with a shallow-water marine nematode ArchiMer
Bellec, Laure; Cambon Bonavita, Marie-anne; Hourdez, Stéphane; Jebbar, Mohamed; Tasiemski, Aurélie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela.
Prokaryotes and free-living nematodes are both very abundant and co-occur in marine environments, but little is known about their possible association. Our objective was to characterize the microbiome of a neglected but ecologically important group of free-living benthic nematodes of the Oncholaimidae family. We used a multi-approach study based on microscopic observations (Scanning Electron Microscopy and Fluorescence In Situ Hybridization) coupled with an assessment of molecular diversity using metabarcoding based on the 16S rRNA gene. All investigated free-living marine nematode specimens harboured distinct microbial communities (from the surrounding water and sediment and through the seasons) with ectosymbiosis seemed more abundant during summer....
Tipo: Text
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00494/60614/64098.pdf
Imagem não selecionada

Imprime registro no formato completo
Combined Whole-Cell High-Throughput Functional Screening for Identification of New Nicotinamidases/Pyrazinamidases in Metagenomic/Polygenomic Libraries ArchiMer
Zapata-perez, Ruben; Garcia-saura, Antonio G.; Jebbar, Mohamed; Golyshin, Peter N.; Sanchez-ferrer, Alvaro.
Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide (NAM) to produce ammonia and nicotinic acid (NA). These enzymes are an essential component of the NAD(+) salvage pathway and are implicated in the viability of several pathogenic organisms. Its absence in humans makes them a promising drug target. In addition, although they are key analytical biocatalysts for screening modulators in relevant biomedical enzymes, such as sirtuins and poly-ADP-ribosyltransferases, no commercial sources are available. Surprisingly, the finding of an affordable source of nicotinamidase from metagenomic libraries is hindered by the absence of a suitable and fast screening method. In this manuscript, we describe the development of two new whole-cell methods...
Tipo: Text Palavras-chave: Amidohydrolases; Nicotinamidase; Functional screening; Metagenome; Library; High-throughput screening assays.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00362/47269/47232.pdf
Imagem não selecionada

Imprime registro no formato completo
Complete genome sequence and whole-genome phylogeny of Kosmotoga pacifica type strain SLHLJ1T from an East Pacific hydrothermal sediment ArchiMer
Jiang, Lijing; L'Haridon, Stephane; Jebbar, Mohamed; Xu, Hongxiu; Alain, Karine; Shao, Zongze.
Kosmotoga pacifica strain SLHLJ1T is a thermophilic chemoorganoheterotrophic bacterium isolated from a deep-sea hydrothermal sediment. It belongs to the physiologically homogeneous Thermotogaceae family. Here, we describe the phenotypic features of K. pacifica together with its genome sequence and annotation. The chromosome has 2,169,170 bp, organized in one contig. A total of 1897 candidate protein-encoding genes and 177 RNA genes were identified. The 16S rRNA gene sequence of this strain is distantly related to sequences of some relatives classified in the same genus (K. olearia 7.02% and K. shengliensis 7.83%), with dissimilarity percentages close to the threshold generally described for genus delineation. Nevertheless, the percentage of conserved...
Tipo: Text Palavras-chave: Marine; Hydrothermal vent; Thermotogales; Chemoorganoheterotroph; Thermophile.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48191/48324.pdf
Imagem não selecionada

Imprime registro no formato completo
Complete Genome Sequence of Hyperthermophilic Piezophilic Archaeon Palaeococcus pacificus DY20341(T), Isolated from Deep-Sea Hydrothermal Sediments ArchiMer
Zeng, Xiang; Jebbar, Mohamed; Shao, Zongze.
We report the genome sequence of Palaeococcus pacificus DY20341(T), isolated from a sediment sample collected from eastern Pacific Ocean hydrothermal fields, which is the first report of a complete genome for a Palaeococcus species. The genome sequence will help to better understand differentiation phylogenetic relationships and evolution of several Thermococcales species.
Tipo: Text
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00601/71312/69741.pdf
Imagem não selecionada

Imprime registro no formato completo
Complete Genome Sequence of Methanohalophilus halophilus DSM 3094 T , Isolated from a Cyanobacterial Mat and Bottom Deposits at Hamelin Pool, Shark Bay, Northwestern Australia ArchiMer
L’haridon, Stéphane; Corre, Erwan; Guan, Yue; Vinu, Manikandan; La Cono, Violetta; Yakimov, Mickail; Stingl, Ulrich; Toffin, Laurent; Jebbar, Mohamed.
The complete genome sequence of Methanohalophilus halophilus DSM 3094T, a member of the Methanosarcinaceae family and the Methanosarcianales order, consists of 2,022,959 bp in one contig and contains 2,137 predicted genes. The genome is consistent with a halophilic methylotrophic anaerobic lifestyle, including the methylotrophic and CO2-H2 methanogensis pathways.
Tipo: Text
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00372/48351/48523.pdf
Imagem não selecionada

Imprime registro no formato completo
Complete Genome Sequence of the Halophilic Methylotrophic Methanogen Archaeon Methanohalophilus portucalensis Strain FDF-1 T ArchiMer
L'Haridon, Stephane; Corre, Erwan; Guan, Yue; Vinu, Manikandan; La Cono, Violetta; Yakimov, Michail; Stingl, Ulrich; Toffin, Laurent; Jebbar, Mohamed.
We report here the complete genome sequence (2.08 Mb) of Methanohalophilus portucalensis strain FDF-1T, a halophilic methylotrophic methanogen isolated from the sediment of a saltern in Figeria da Foz, Portugal. The average nucleotide identity and DNA-DNA hybridization analyses show that Methanohalophilus mahii, M. halophilus, and M. portucalensis are three different species within the Methanosarcinaceae family.
Tipo: Text
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00420/53138/54102.pdf
Imagem não selecionada

Imprime registro no formato completo
Complete Genome Sequence of the Hyperthermophilic Piezophilic Archaeon Pyrococcus kukulkanii NCB100 Isolated from the Rebecca’s Roost Hydrothermal Vent in the Guaymas Basin ArchiMer
Oger, Philippe M.; Callac, Nolwenn; Oger-desfeux, Christine; Hughes, Sandrine; Gillet, Benjamin; Jebbar, Mohamed; Godfroy, Anne.
Members of the order Thermococcales are common inhabitants of high-temperature hydrothermal vent systems (black smokers) that are represented in clone libraries mostly by isolates from the Thermococcus genus. We report the complete sequence of a novel species from the Pyrococcus genus, P. kukulkanii strain NCB100, which has been isolated from a flange fragment of the Rebecca’s Roost hydrothermal vent system in the Guaymas Basin.
Tipo: Text
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00476/58798/61335.pdf
Imagem não selecionada

Imprime registro no formato completo
Complete Genome Sequence of the Hyperthermophilic, Piezophilic, Heterotrophic, and Carboxydotrophic Archaeon Thermococcus barophilus MP ArchiMer
Vannier, Pauline; Marteinsson, Viggo Thor; Fridjonsson, Olafur Hedinn; Oger, Philippe; Jebbar, Mohamed.
Thermococcus barophilus is a hyperthermophilic, anaerobic, mixed heterotrophic, and carboxydotrophic euryarchaeon isolated from the deep sea hydrothermal vent Snakepit site on the mid-Atlantic ridge at a depth of 3,550 m. T. barophilus is the first true piezophilic, hyperthermophilic archaeon isolated, having an optimal growth at 40 MPa. Here we report the complete genome sequence of strain MP, the type strain of T. barophilus. The genome data reveal a close proximity with Thermococcus sibiricus, another Thermococcus isolated from the deep biosphere and a possible connection to life in the depths.
Tipo: Text
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14185/11865.pdf
Imagem não selecionada

Imprime registro no formato completo
Complete Genome Sequence of the Obligate Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii CH1 ArchiMer
Jun, Xu; Liu Lupeng,; Xu Minjuan,; Oger, Philippe; Wang Fengping,; Jebbar, Mohamed; Xiang, Xiao.
Pyrococcus yayanosii CH1 is the first obligate piezophilic hyperthermophilic archaeon isolated from the deep-sea hydrothermal site Ashadze on the mid-Atlantic ridge at a depth of 4,100 m. This organism grows within a temperature range of 80 to 108 degrees C and a hydrostatic pressure range of 20 to 120 MPa, with optima at 98 degrees C and 52 MPa, respectively. Here, we report the complete genome sequence (1,716,817 bp, with a G+C content of 51.6%) of the type strain P. yayanosii CH1(T) (= JCM 16557). This genomic information reveals a systematic view of the piezoadaptation strategy and evolution scenario of metabolic pathways in Thermococcales.
Tipo: Text
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00041/15247/12621.pdf
Imagem não selecionada

Imprime registro no formato completo
Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure ArchiMer
Peters, Judith; Martinez, Nicolas; Michoud, Gregoire; Carlo, Anais; Franzetti, Bruno; Oger, Philippe; Jebbar, Mohamed.
The majority of the biosphere is a high pressure environment. Around 70% of the marine biosphere lies at depths below 1000 m, i.e. at pressures of 100 bars or higher. To survive in these environments, deep-biosphere organisms have adapted to life at high pressure. In vitro studies showed that the activity of certain proteins originating from deep-sea organisms is less affected by high pressure than that of enzymes from surface organisms [1-3]. However, the genetic and structural bases for this increased pressure resistance are still unknown. Elastic incoherent neutron scattering studies, which provide access to information about molecular dynamics, constitute a very promising approach to decipher the structural adaptation in proteins living under high...
Tipo: Text Palavras-chave: Neutron Scattering; Molecular Dynamics; High Pressure; Deep Sea Microbes.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00251/36186/34725.pdf
Imagem não selecionada

Imprime registro no formato completo
Deep sea, the last great unexplored Earth frontier harboring the largest unknown and untapped remote microbial diversity on our planet ArchiMer
Jebbar, Mohamed.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00277/38850/37389.pdf
Imagem não selecionada

Imprime registro no formato completo
Desulfurobacterium indicum sp nov., a thermophilic sulfur-reducing bacterium from the Indian Ocean ArchiMer
Cao, Junwei; Birien, Tiphaine; Gayet, Nicolas; Huang, Zhaobin; Shao, Zongze; Jebbar, Mohamed; Alain, Karine.
A novel sulfur-reducing bacterium, strain K6013(T), was isolated from a sulfide sample collected at a depth of 2771 m from a high-temperature hydrothermal vent in the Indian Ocean. Cells were Gram-stain-negative, anaerobic, motile rods (0.92.2 x 0.4-0.6 mu m). The strain grew at NaCl concentrations ranging from 1 to 4.5% (w/v) (optimum 2.5 %), at pH 5 to 8 (optimum pH 6), and at temperatures between 40 and 75 degrees C (optimum 65 degrees C). K6013(T) was an obligate chemolithoautotroph, using thiosulfate, sulfur and nitrate as terminal electron acceptors in the presence of H-2 but not sulfate, sulfite nor nitrite. The major cellular fatty acids were C-16 : 0 (17.4 %), C-18 : 1 omega 7c/C-18 : 1 omega 6c (ummed feature 8, 37.91 %), C-18 : 0 (18.29 %) and...
Tipo: Text Palavras-chave: Desulfurobacterium; Thermophilic; Hydrothermal vent; Chemolithoautotroph; Sulfur-reducer.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00395/50637/53724.pdf
Imagem não selecionada

Imprime registro no formato completo
Development of an Effective 6-Methylpurine Counterselection Marker for Genetic Manipulation in Thermococcus barophilus ArchiMer
Birien, Tiphaine; Thiel, Axel; Henneke, Ghislaine; Flament, Didier; Moalic, Yann; Jebbar, Mohamed.
A gene disruption system for Thermococcus barophilus was developed using simvastatin (HMG-CoA reductase encoding gene) for positive selection and 5-Fluoroorotic acid (5-FOA), a pyrF gene for negative selection. Multiple gene mutants were constructed with this system, which offers the possibility of complementation in trans, but produces many false positives (<80%). To significantly reduce the rate of false positives, we used another counterselective marker, 6-methylpurine (6-MP), a toxic analog of adenine developed in Thermococcus kodakarensis, consistently correlated with the TK0664 gene (encoding a hypoxanthine-guanine phosphoribosyl-transferase). We thus replaced pyrF by TK0664 on our suicide vector and tested T. barophilus strain sensitivity to 6-MP...
Tipo: Text Palavras-chave: Archaea; Piezophiles; Hyperthermophiles; Genetics; Gene deletion; Deep sea; Hydrothermal vents.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00425/53679/54523.pdf
Imagem não selecionada

Imprime registro no formato completo
DNA extractions from deep subseafloor sediments: Novel cryogenic-mill-based procedure and comparison to existing protocols ArchiMer
Alain, Karine; Callac, Nolwenn; Ciobanu, Maria Cristina; Reynaud, Yann; Duthoit, Frederique; Jebbar, Mohamed.
Extracting DNA from deep subsurface sediments is challenging given the complexity of sediments types, low bio-masses, resting structures (spores, cysts) frequently encountered in deep sediments, and the potential presence of enzymatic inhibitors. Promising results for cell lysis efficiency were recently obtained by use of a cryogenic mill (Lipp et al., 2008). These findings encouraged us to devise a DNA extraction protocol using this tool. Thirteen procedures involving a combination of grinding in liquid nitrogen (for various durations and beating rates) with different chemical solutions (phenol, chloroform, SDS, sarkosyl, proteinase, GTC), or with use of DNA recovery kits (MagExtractor (R)) were compared. Effective DNA extraction was evaluated in terms of...
Tipo: Text Palavras-chave: Sediment; DNA extraction; Deep subsurface biosphere.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00056/16691/14364.pdf
Imagem não selecionada

Imprime registro no formato completo
Draft Genome of Halomonas lionensis RHS90 T , a Stress-Tolerant Gammaproteobacterium Isolated from Mediterranean Sea Sediments ArchiMer
Gaboyer, Frédéric; Maignien, Loïs; Jebbar, Mohamed; Alain, Karine.
Members of the genus Halomonas are physiologically versatile and harbor ecological adaptations enabling the colonization of contrasted environments. We present here the draft genome of Halomonas lionensis RHS90T, isolated from Mediterranean Sea sediments. Numerous genes related to stress tolerance, DNA repair, or external signal-sensing systems were predicted, which could represent selective advantages of this marine bacterium.
Tipo: Text
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00388/49906/50477.pdf
Imagem não selecionada

Imprime registro no formato completo
Draft Genome Sequence of Phaeobacter leonis Type Strain 306, an Alphaproteobacterium Isolated from Mediterranean Sea Sediments ArchiMer
Gaboyer, Frédéric; Malgnien, Loïs; Jebbar, Mohamed; Alain, Karine.
Phaeobacter leonis strain 306T is an alphaproteobacterium isolated from Mediterranean Sea sediments. It belongs to the genus Phaeobacter, which was recently proposed and is still poorly characterized. In an effort to better understand the fundamental aspects of the microbiology of this genus, we present here the 4.82-Mb draft genome sequence of Phaeobacter leonis strain 306T.
Tipo: Text
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00390/50186/50809.pdf
Imagem não selecionada

Imprime registro no formato completo
Electroactive Bacteria Associated With Stainless Steel Ennoblement in Seawater ArchiMer
Trigodet, Florian; Larche, Nicolas; Morrison, Hilary G.; Jebbar, Mohamed; Thierry, Dominique; Maignien, Lois.
Microorganisms can increase the open-circuit potential of stainless steel immersed in seawater of several hundred millivolts in a phenomenon called ennoblement. It raises the chance of corrosion as the open-circuit potential may go over the pitting corrosion potential. Despite the large impact of the ennoblement, no unifying mechanisms have been described as responsible for the phenomenon. Here we show that the strict electrotroph bacterium "Candidatus Tenderia electrophaga" is detected as an ennoblement biomarker and is only present at temperatures at which we observe ennoblement. This bacterium was previously enriched in biocathode systems. Our results suggest that "Candidatus Tenderia electrophaga," and its previously described extracellular electron...
Tipo: Text Palavras-chave: Ennoblement; Electroactive bacteria; Stainless steel; Microbial ecology; 16S rRNA gene.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00482/59371/62144.pdf
Imagem não selecionada

Imprime registro no formato completo
Functional Screening of Hydrolytic Activities Reveals an Extremely Thermostable Cellulase from a Deep-Sea Archaeon ArchiMer
Leis, Benedikt; Heinze, Simon; Angelov, Angel; Pham, Vu Thuy Trang; Thürmer, Andrea; Jebbar, Mohamed; Golyshin, Peter N.; Streit, Wolfgang R.; Daniel, Rolf; Liebl, Wolfgang.
Extreme habitats serve as a source of enzymes that are active under extreme conditions and are candidates for industrial applications. In this work, six large-insert mixed genomic libraries were screened for hydrolase activities in a broad temperature range (8–70°C). Among a variety of hydrolytic activities, one fosmid clone, derived from a library of pooled isolates of hyperthermophilic archaea from deep sea vents, displayed hydrolytic activity on carboxymethyl cellulose substrate plates at 70°C but not at lower temperatures. Sequence analysis of the fosmid insert revealed a gene encoding a novel glycoside hydrolase family 12 (GHF12) endo-1,4-β-glucanase, termed Cel12E. The enzyme shares 45% sequence identity with a protein from the archaeon Thermococcus...
Tipo: Text Palavras-chave: Functional screenings; Extreme thermostable protein; Archaeal endoglucanase; Enzymatic characterization.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00601/71319/69744.pdf
Imagem não selecionada

Imprime registro no formato completo
Genetic Manipulations of the Hyperthermophilic Piezophilic Archaeon Thermococcus barophilus ArchiMer
Thiel, Axel; Michoud, Gregoire; Moalic, Yann; Flament, Didier; Jebbar, Mohamed.
In this study, we developed a gene disruption system for Thermococcus barophilus using simvastatin for positive selection and 5-fluoroorotic acid (5-FOA) for negative selection or counterselection to obtain markerless deletion mutants using single-and double-crossover events. Disruption plasmids carrying flanking regions of each targeted gene were constructed and introduced by transformation into wild-type T. barophilus MP cells. Initially, a pyrF deletion mutant was obtained as a starting point for the construction of further markerless mutants. A deletion of the hisB gene was also constructed in the UBOCC-3256 (Delta pyrF) background, generating a strain (UBOCC-3260) that was auxotrophic for histidine. A functional pyrF or hisB allele from T. barophilus...
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00187/29818/28263.pdf
Registros recuperados: 39
Primeira ... 12 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional