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Bacterial diversity in bovine rumen by metagenomic 16S rDNA sequencing and scanning electron microscopy Animal Sciences
Jesus, Raphael Barbetta de; Omori, Wellington Pine; Lemos, Eliana Gertrudes de Macedo; Souza, Jackson Antônio Marcondes de.
The bacterial diversity by 16S rDNA partial sequencing and scanning electron microscope (SEM) of the rumen microbiome was characterized. Three Nellore bovines, cannulated at the rumen, were utilized. Liquid and solid fractions from the rumen content were processed for the extraction of metagenomic DNA and later 16S rDNA amplicons were utilized to construct the WGA library for further clone sequencing. Data were analyzed by MEGA and MOTUR (University of Michigan) softwares. Approximately 97.96% of operation taxonomic units (OTUs) were related to Bacteriodetes phylum and 2.04% of sequences were affiliated to Firmicutes phylum. In the case of Bacteriodetes, the great part of sequences (47.96%) was attributed to Prevotella genus. Bacteroidetes phylum was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Área (Zootecnia - 5.04.00.00-2) Sub-área (Exigências Nutricionais dos Animais - 5.04.03.01-0) bacterial community; Bacterioidetes; Nellore; Prevotella; Taxonomic affiliation.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/26535
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